ENSG00000127824

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000248437 ENSG00000127824 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBA4A protein_coding protein_coding 6.423658 13.09306 1.860823 0.470827 0.0495924 -2.808159 4.9428371 10.15149 1.860823 0.1553080 0.0495924 -2.441367 0.7897542 0.7781333 1 0.2218667 4.057205e-08 4.057205e-08 FALSE TRUE
ENST00000498660 ENSG00000127824 HEK293_OSMI2_2hA HEK293_TMG_2hB TUBA4A protein_coding processed_transcript 6.423658 13.09306 1.860823 0.470827 0.0495924 -2.808159 0.9309317 2.14128 0.000000 0.4581668 0.0000000 -7.749051 0.1172917 0.1614667 0 -0.1614667 1.205679e-06 4.057205e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000127824 E001 0.0000000       2 219249710 219249710 1 -      
ENSG00000127824 E002 139.4010780 0.0015500217 0.3691637990 0.513603543 2 219249711 219250728 1018 - 1.896 1.932 0.121
ENSG00000127824 E003 55.9659576 0.0046834516 0.3951104437 0.538949125 2 219250729 219250771 43 - 1.484 1.547 0.217
ENSG00000127824 E004 139.4145051 0.0004295967 0.8355227626 0.897132012 2 219250772 219251112 341 - 1.925 1.927 0.009
ENSG00000127824 E005 47.6774144 0.0045570643 0.2780457778 0.417671390 2 219251113 219251172 60 - 1.384 1.472 0.303
ENSG00000127824 E006 32.7975318 0.0006724421 0.4697125689 0.608933627 2 219251173 219251193 21 - 1.255 1.318 0.221
ENSG00000127824 E007 69.2874382 0.0003995202 0.0740886691 0.150071381 2 219251194 219251323 130 - 1.521 1.637 0.395
ENSG00000127824 E008 1.3243307 0.0102909269 0.4142079655 0.557611646 2 219251324 219251538 215 - 0.000 0.263 11.701
ENSG00000127824 E009 0.9880035 0.1785696148 0.6892465065 0.791152007 2 219251539 219251564 26 - 0.000 0.206 10.287
ENSG00000127824 E010 41.6067858 0.0005993647 0.0933516912 0.180359980 2 219251565 219251586 22 - 1.276 1.421 0.505
ENSG00000127824 E011 71.7045361 0.0008355277 0.5850773483 0.708847054 2 219251587 219251713 127 - 1.676 1.636 -0.139
ENSG00000127824 E012 0.1482932 0.0414518848 0.7437850916   2 219251714 219252007 294 - 0.000 0.049 8.670
ENSG00000127824 E013 92.1940037 0.0105156940 0.0273714231 0.066748629 2 219252008 219252230 223 - 1.881 1.730 -0.512
ENSG00000127824 E014 0.5911836 0.0342321480 1.0000000000 1.000000000 2 219252779 219252995 217 - 0.000 0.132 10.301
ENSG00000127824 E015 0.2214452 0.0400300425 0.7492579682   2 219252996 219253145 150 - 0.000 0.049 8.688
ENSG00000127824 E016 0.0000000       2 219253209 219253354 146 -      
ENSG00000127824 E017 46.5472904 0.0032175971 0.0005652653 0.002403713 2 219253856 219253918 63 - 1.676 1.424 -0.864
ENSG00000127824 E018 0.4782907 0.0216627920 1.0000000000 1.000000000 2 219254095 219254608 514 - 0.000 0.132 10.413
ENSG00000127824 E019 0.2965864 0.1557393958 1.0000000000   2 219277814 219277902 89 - 0.000 0.091 9.005