ENSG00000127663

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000159111 ENSG00000127663 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM4B protein_coding protein_coding 16.84404 23.47606 9.919026 2.20889 0.3090521 -1.242081 2.091797 4.123885 1.629225 0.8488639 0.1486175 -1.3344849 0.1227750 0.1715000 0.1647333 -0.006766667 9.670745e-01 1.163237e-30 FALSE TRUE
ENST00000536461 ENSG00000127663 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM4B protein_coding protein_coding 16.84404 23.47606 9.919026 2.20889 0.3090521 -1.242081 3.919655 9.697859 0.000000 0.4613802 0.0000000 -9.9230094 0.1781042 0.4201667 0.0000000 -0.420166667 1.163237e-30 1.163237e-30 FALSE TRUE
ENST00000611640 ENSG00000127663 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM4B protein_coding protein_coding 16.84404 23.47606 9.919026 2.20889 0.3090521 -1.242081 7.138116 5.530313 6.343331 0.7737199 0.6324600 0.1975457 0.4744000 0.2348000 0.6371000 0.402300000 2.459660e-08 1.163237e-30 FALSE TRUE
MSTRG.16238.4 ENSG00000127663 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM4B protein_coding   16.84404 23.47606 9.919026 2.20889 0.3090521 -1.242081 2.449248 2.725661 1.299230 0.5270975 0.3088007 -1.0631708 0.1479000 0.1142667 0.1332333 0.018966667 9.007791e-01 1.163237e-30 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000127663 E001 0.1817044 0.0398059792 1.000000e+00   19 4969113 4969113 1 + 0.000 0.087 16.341
ENSG00000127663 E002 0.1817044 0.0398059792 1.000000e+00   19 4969114 4969116 3 + 0.000 0.087 16.335
ENSG00000127663 E003 5.9229867 0.0027772863 2.681564e-02 6.560948e-02 19 4969117 4969131 15 + 0.961 0.716 -0.957
ENSG00000127663 E004 33.9119730 0.0008752913 1.485736e-06 1.189336e-05 19 4969132 4969230 99 + 1.659 1.403 -0.874
ENSG00000127663 E005 55.3471071 0.0004861611 1.749374e-08 2.062494e-07 19 5016257 5016324 68 + 1.851 1.618 -0.791
ENSG00000127663 E006 44.9406320 0.0007123493 7.210344e-10 1.099113e-08 19 5016325 5016339 15 + 1.795 1.505 -0.987
ENSG00000127663 E007 93.5799943 0.0003567716 5.065856e-08 5.454455e-07 19 5032866 5033031 166 + 2.037 1.870 -0.560
ENSG00000127663 E008 123.7499403 0.0002633839 6.436898e-07 5.569541e-06 19 5039836 5040011 176 + 2.128 2.003 -0.418
ENSG00000127663 E009 125.9600124 0.0002392485 1.100368e-09 1.618741e-08 19 5041137 5041251 115 + 2.158 1.999 -0.532
ENSG00000127663 E010 0.4782907 0.0253224738 3.256768e-01 4.691259e-01 19 5046998 5047140 143 + 0.000 0.220 17.915
ENSG00000127663 E011 178.0759280 0.0002460517 2.624440e-09 3.601277e-08 19 5047476 5047669 194 + 2.284 2.159 -0.418
ENSG00000127663 E012 0.0000000       19 5070618 5071009 392 +      
ENSG00000127663 E013 77.1961382 0.0027277465 3.323009e-01 4.759355e-01 19 5071010 5071059 50 + 1.839 1.835 -0.013
ENSG00000127663 E014 4.0546691 0.0041689898 5.839694e-01 7.079766e-01 19 5074091 5077366 3276 + 0.684 0.635 -0.209
ENSG00000127663 E015 117.2130210 0.0045907050 6.600769e-03 2.018785e-02 19 5077367 5077470 104 + 2.075 1.994 -0.272
ENSG00000127663 E016 32.9509462 0.0006490785 2.446908e-02 6.078908e-02 19 5077471 5079166 1696 + 1.325 1.528 0.700
ENSG00000127663 E017 3.0925020 0.0051617948 1.052484e-01 1.984727e-01 19 5080688 5080996 309 + 0.725 0.506 -0.966
ENSG00000127663 E018 104.2275972 0.0007371867 2.456761e-05 1.493186e-04 19 5082367 5082451 85 + 2.048 1.930 -0.396
ENSG00000127663 E019 92.3169655 0.0045732825 2.378017e-02 5.936411e-02 19 5082452 5082504 53 + 1.962 1.896 -0.223
ENSG00000127663 E020 157.3274492 0.0028270353 7.235789e-04 2.983306e-03 19 5110622 5110818 197 + 2.204 2.122 -0.277
ENSG00000127663 E021 0.3729606 0.0311640763 5.226516e-01 6.564182e-01 19 5111375 5111544 170 + 0.170 0.087 -1.115
ENSG00000127663 E022 6.9106825 0.0024984247 8.534607e-02 1.679675e-01 19 5111775 5112357 583 + 0.639 0.919 1.119
ENSG00000127663 E023 4.7823327 0.1778957538 6.300159e-01 7.452732e-01 19 5112358 5112636 279 + 0.796 0.703 -0.377
ENSG00000127663 E024 3.8611356 0.0040406679 9.171489e-01 9.516489e-01 19 5112637 5113340 704 + 0.639 0.656 0.075
ENSG00000127663 E025 94.7071469 0.0003236530 1.146751e-01 2.123580e-01 19 5119118 5119219 102 + 1.941 1.921 -0.066
ENSG00000127663 E026 137.8981847 0.0002809245 1.002935e-01 1.909270e-01 19 5119653 5119852 200 + 2.096 2.085 -0.037
ENSG00000127663 E027 241.8135366 0.0002228685 2.826331e-02 6.853018e-02 19 5131076 5131545 470 + 2.338 2.327 -0.038
ENSG00000127663 E028 101.0264413 0.0011759098 6.657237e-02 1.376774e-01 19 5131887 5132007 121 + 1.975 1.944 -0.104
ENSG00000127663 E029 127.8392557 0.0041699395 8.909568e-01 9.343690e-01 19 5133883 5134061 179 + 2.029 2.069 0.133
ENSG00000127663 E030 161.9206302 0.0002933538 9.190061e-01 9.528349e-01 19 5135339 5135535 197 + 2.128 2.169 0.136
ENSG00000127663 E031 70.5105978 0.0003694066 4.472210e-01 5.886344e-01 19 5135536 5135561 26 + 1.742 1.821 0.267
ENSG00000127663 E032 115.6663811 0.0002648284 3.479407e-01 4.921552e-01 19 5137262 5137338 77 + 1.955 2.033 0.263
ENSG00000127663 E033 99.3262116 0.0003279003 1.702342e-01 2.884843e-01 19 5137621 5137676 56 + 1.871 1.971 0.334
ENSG00000127663 E034 134.2560940 0.0002726034 3.244596e-02 7.671130e-02 19 5137962 5138070 109 + 1.984 2.103 0.400
ENSG00000127663 E035 0.4428904 0.4052620573 6.826841e-01 7.860818e-01 19 5138262 5138470 209 + 0.000 0.165 17.403
ENSG00000127663 E036 14.4527280 0.0061090650 6.501592e-04 2.716661e-03 19 5140252 5141503 1252 + 0.763 1.218 1.696
ENSG00000127663 E037 201.7752889 0.0015365713 1.360571e-01 2.425506e-01 19 5143967 5144152 186 + 2.179 2.276 0.323
ENSG00000127663 E038 236.8469983 0.0001915006 8.214507e-01 8.874233e-01 19 5144248 5144412 165 + 2.293 2.330 0.127
ENSG00000127663 E039 208.3288736 0.0002524851 2.602076e-01 3.976835e-01 19 5144783 5144902 120 + 2.210 2.285 0.252
ENSG00000127663 E040 180.4550823 0.0002066070 8.823619e-02 1.724255e-01 19 5150358 5150450 93 + 2.133 2.228 0.317
ENSG00000127663 E041 1158.1709543 0.0050652574 1.181391e-11 2.430086e-10 19 5151335 5153598 2264 + 2.818 3.071 0.843