ENSG00000127527

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000248070 ENSG00000127527 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15L1 protein_coding protein_coding 27.22862 37.66827 18.13889 2.105229 0.3828199 -1.053852 2.6317937 3.9866805 0.2764947 1.3501628 0.2764947 -3.8022217 0.08569167 0.10316667 0.01466667 -0.0885000000 0.087665366 0.004534522 FALSE TRUE
ENST00000455140 ENSG00000127527 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15L1 protein_coding protein_coding 27.22862 37.66827 18.13889 2.105229 0.3828199 -1.053852 6.1086829 9.1768542 3.7705352 0.3036633 0.4439829 -1.2809804 0.22130000 0.24516667 0.20786667 -0.0373000000 0.613009101 0.004534522 FALSE TRUE
ENST00000535753 ENSG00000127527 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15L1 protein_coding protein_coding 27.22862 37.66827 18.13889 2.105229 0.3828199 -1.053852 5.5833469 7.9826716 3.9071410 0.7864824 0.6405167 -1.0288767 0.20156250 0.21370000 0.21416667 0.0004666667 1.000000000 0.004534522 FALSE TRUE
MSTRG.16633.4 ENSG00000127527 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15L1 protein_coding   27.22862 37.66827 18.13889 2.105229 0.3828199 -1.053852 7.3175070 8.6606622 6.2537704 0.6873495 0.3631528 -0.4691109 0.28486667 0.22960000 0.34530000 0.1157000000 0.004534522 0.004534522 FALSE TRUE
MSTRG.16633.5 ENSG00000127527 HEK293_OSMI2_2hA HEK293_TMG_2hB EPS15L1 protein_coding   27.22862 37.66827 18.13889 2.105229 0.3828199 -1.053852 0.7148165 0.6928024 1.6325244 0.3312365 0.4154830 1.2247237 0.03036250 0.01753333 0.09013333 0.0726000000 0.089571397 0.004534522 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000127527 E001 0.0000000       19 16355239 16355246 8 -      
ENSG00000127527 E002 37.2593517 0.0211629823 9.674189e-02 1.854931e-01 19 16355247 16355411 165 - 1.396 1.564 0.579
ENSG00000127527 E003 106.5592941 0.0119828088 5.257380e-02 1.137422e-01 19 16355412 16355586 175 - 1.873 2.008 0.453
ENSG00000127527 E004 232.3935605 0.0040190738 1.043799e-03 4.110261e-03 19 16355587 16355851 265 - 2.206 2.343 0.457
ENSG00000127527 E005 6.0505505 0.0057335102 9.293948e-01 9.595847e-01 19 16361579 16361580 2 - 0.811 0.801 -0.040
ENSG00000127527 E006 8.0496265 0.0640687071 7.968165e-01 8.703457e-01 19 16361581 16361596 16 - 0.873 0.923 0.189
ENSG00000127527 E007 69.9687256 0.0268723817 4.557325e-05 2.593642e-04 19 16361597 16361778 182 - 1.453 1.873 1.429
ENSG00000127527 E008 158.5359052 0.0048996653 3.649432e-10 5.857613e-09 19 16361779 16361885 107 - 1.895 2.211 1.056
ENSG00000127527 E009 79.8412262 0.0030578858 9.809440e-06 6.535039e-05 19 16361886 16361888 3 - 1.661 1.907 0.831
ENSG00000127527 E010 183.6949386 0.0031840091 5.624094e-08 5.992507e-07 19 16361889 16361984 96 - 2.029 2.260 0.773
ENSG00000127527 E011 139.4977594 0.0035952387 1.089727e-05 7.178467e-05 19 16377122 16377254 133 - 1.927 2.135 0.698
ENSG00000127527 E012 39.3408038 0.0007078500 3.944898e-04 1.753517e-03 19 16382989 16384275 1287 - 1.359 1.602 0.833
ENSG00000127527 E013 238.5411630 0.0007608021 2.723652e-06 2.058270e-05 19 16385129 16385211 83 - 2.214 2.354 0.469
ENSG00000127527 E014 234.9692054 0.0027171801 6.619002e-02 1.370303e-01 19 16386171 16386231 61 - 2.261 2.332 0.235
ENSG00000127527 E015 301.6063312 0.0006581088 9.180389e-02 1.779503e-01 19 16392304 16392440 137 - 2.389 2.435 0.152
ENSG00000127527 E016 183.2337337 0.0005014106 7.941342e-01 8.684554e-01 19 16393951 16394001 51 - 2.214 2.208 -0.018
ENSG00000127527 E017 223.4762018 0.0001998164 8.178183e-01 8.848119e-01 19 16395344 16395467 124 - 2.288 2.296 0.027
ENSG00000127527 E018 78.0049136 0.0006715091 9.303340e-07 7.772650e-06 19 16400631 16402314 1684 - 1.651 1.896 0.828
ENSG00000127527 E019 5.4109890 0.0071260915 6.927166e-02 1.421526e-01 19 16402315 16402320 6 - 0.542 0.829 1.207
ENSG00000127527 E020 304.9013094 0.0001516966 3.354418e-02 7.886258e-02 19 16402321 16402485 165 - 2.389 2.439 0.169
ENSG00000127527 E021 377.3501026 0.0001401059 6.875647e-01 7.898772e-01 19 16403733 16403930 198 - 2.524 2.518 -0.020
ENSG00000127527 E022 309.6062289 0.0001765577 4.475820e-04 1.957682e-03 19 16404588 16404749 162 - 2.488 2.413 -0.250
ENSG00000127527 E023 209.2825729 0.0002037404 4.922550e-04 2.130646e-03 19 16413773 16413845 73 - 2.328 2.237 -0.303
ENSG00000127527 E024 0.0000000       19 16417439 16417551 113 -      
ENSG00000127527 E025 211.4113760 0.0008391202 5.358588e-04 2.294062e-03 19 16417552 16417637 86 - 2.338 2.240 -0.326
ENSG00000127527 E026 261.7427974 0.0028942880 2.827080e-03 9.755055e-03 19 16417948 16418104 157 - 2.431 2.333 -0.324
ENSG00000127527 E027 237.3994757 0.0002083956 8.259498e-07 6.983563e-06 19 16421319 16421476 158 - 2.403 2.283 -0.401
ENSG00000127527 E028 97.3172768 0.0046446068 2.955762e-03 1.013180e-02 19 16425083 16425091 9 - 2.037 1.893 -0.484
ENSG00000127527 E029 189.8459140 0.0007654147 1.691374e-05 1.066486e-04 19 16425092 16425316 225 - 2.309 2.184 -0.421
ENSG00000127527 E030 95.5103797 0.0044337107 1.716104e-01 2.903059e-01 19 16428702 16428761 60 - 1.971 1.906 -0.218
ENSG00000127527 E031 152.8758440 0.0014137015 1.608347e-03 5.988179e-03 19 16434365 16434483 119 - 2.203 2.095 -0.361
ENSG00000127527 E032 73.1233314 0.0004188883 5.409786e-03 1.704072e-02 19 16434484 16434490 7 - 1.895 1.774 -0.410
ENSG00000127527 E033 0.4386386 0.1500839311 2.257773e-01 3.573097e-01 19 16434491 16435296 806 - 0.299 0.083 -2.233
ENSG00000127527 E034 0.4407149 0.0229190159 1.940107e-01 3.188246e-01 19 16436596 16436936 341 - 0.300 0.084 -2.225
ENSG00000127527 E035 136.3743101 0.0028964925 1.226757e-02 3.417426e-02 19 16436937 16436999 63 - 2.148 2.049 -0.332
ENSG00000127527 E036 0.1515154 0.0421480198 2.070519e-01   19 16437000 16437064 65 - 0.175 0.000 -9.987
ENSG00000127527 E037 162.4795121 0.0047533587 8.539906e-02 1.680561e-01 19 16437770 16437865 96 - 2.207 2.135 -0.239
ENSG00000127527 E038 4.0062170 0.0041341921 1.409743e-02 3.844692e-02 19 16440653 16440861 209 - 0.300 0.738 2.168
ENSG00000127527 E039 110.6843087 0.0079488145 4.213783e-01 5.645286e-01 19 16440862 16440884 23 - 2.021 1.980 -0.135
ENSG00000127527 E040 113.6799753 0.0171047412 4.872745e-01 6.249540e-01 19 16440885 16440909 25 - 2.034 1.992 -0.142
ENSG00000127527 E041 0.8115992 0.0159713777 1.694677e-01 2.874976e-01 19 16441511 16441891 381 - 0.397 0.154 -1.808
ENSG00000127527 E042 149.9772724 0.0175773037 1.874148e-01 3.104739e-01 19 16441892 16441981 90 - 2.183 2.099 -0.281
ENSG00000127527 E043 95.7987823 0.0119283625 1.234333e-02 3.435702e-02 19 16442178 16442219 42 - 2.039 1.883 -0.521
ENSG00000127527 E044 77.9342289 0.0095570194 2.592201e-03 9.041919e-03 19 16471913 16472085 173 - 1.968 1.784 -0.619