ENSG00000127415

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000247933 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding protein_coding 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.3077492 0.0000000 0.5414033 0.00000000 0.10957976 5.7850361 0.07145417 0.00000000 0.16260000 0.16260000 3.078068e-05 6.115146e-09 FALSE TRUE
ENST00000506561 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding retained_intron 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 1.1974967 0.7945165 0.6878729 0.18323343 0.22384554 -0.2051576 0.23702083 0.20443333 0.16960000 -0.03483333 8.030233e-01 6.115146e-09 FALSE TRUE
ENST00000509744 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding processed_transcript 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.3663602 0.4366168 0.2622345 0.15728116 0.18835145 -0.7141874 0.09515000 0.11316667 0.09226667 -0.02090000 6.864352e-01 6.115146e-09   FALSE
ENST00000509948 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding processed_transcript 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.4106165 0.7097176 0.1778789 0.13864275 0.07310541 -1.9376278 0.09620000 0.18566667 0.05710000 -0.12856667 4.963623e-02 6.115146e-09 FALSE FALSE
ENST00000514224 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding protein_coding 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.1521377 0.5161452 0.0000000 0.08597326 0.00000000 -5.7173891 0.03772917 0.14116667 0.00000000 -0.14116667 6.115146e-09 6.115146e-09 FALSE TRUE
ENST00000514417 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding retained_intron 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.7809363 0.3474096 1.2036591 0.18573129 0.60308871 1.7637129 0.17771667 0.09896667 0.27433333 0.17536667 8.092821e-01 6.115146e-09   FALSE
ENST00000514698 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding retained_intron 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.3625531 0.1887652 0.3665590 0.01065953 0.05784141 0.9218107 0.06732917 0.05246667 0.09923333 0.04676667 2.562976e-01 6.115146e-09 TRUE TRUE
ENST00000652070 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding processed_transcript 4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.2995112 0.4513393 0.0000000 0.11215528 0.00000000 -5.5277563 0.08430000 0.12233333 0.00000000 -0.12233333 5.765665e-07 6.115146e-09 FALSE TRUE
MSTRG.24432.13 ENSG00000127415 HEK293_OSMI2_2hA HEK293_TMG_2hB IDUA protein_coding   4.629698 3.803265 3.794894 0.6857822 0.592352 -0.0031702 0.5511044 0.2702146 0.4277925 0.14576875 0.16492686 0.6437150 0.09784583 0.06186667 0.10506667 0.04320000 6.829602e-01 6.115146e-09 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000127415 E001 0.5891098 0.019387492 2.285893e-02 5.744662e-02 4 986997 987043 47 + 0.396 0.000 -13.951
ENSG00000127415 E002 1.3233924 0.023733349 3.807988e-04 1.700515e-03 4 987044 987065 22 + 0.638 0.000 -15.123
ENSG00000127415 E003 1.7673295 0.010809425 2.731003e-05 1.640550e-04 4 987066 987075 10 + 0.737 0.000 -15.539
ENSG00000127415 E004 2.0597512 0.014344708 6.376894e-06 4.432521e-05 4 987076 987086 11 + 0.793 0.000 -15.761
ENSG00000127415 E005 5.7479042 0.003535675 1.417975e-02 3.863306e-02 4 987087 987242 156 + 0.980 0.646 -1.322
ENSG00000127415 E006 10.5796139 0.001860247 4.245219e-01 5.674800e-01 4 987809 987911 103 + 1.098 0.993 -0.383
ENSG00000127415 E007 5.5670375 0.003887578 8.907614e-01 9.342225e-01 4 987912 987914 3 + 0.793 0.788 -0.020
ENSG00000127415 E008 7.0783858 0.002558759 6.516756e-01 7.624864e-01 4 987915 987949 35 + 0.865 0.894 0.114
ENSG00000127415 E009 5.2451173 0.069411410 3.032301e-01 4.451283e-01 4 989231 989623 393 + 0.673 0.833 0.643
ENSG00000127415 E010 1.3995867 0.095647130 2.992013e-01 4.406963e-01 4 991825 991871 47 + 0.242 0.434 1.205
ENSG00000127415 E011 3.0099965 0.005746056 6.901980e-02 1.417470e-01 4 991872 991940 69 + 0.396 0.673 1.317
ENSG00000127415 E012 6.5724799 0.002884548 8.925447e-04 3.588191e-03 4 991941 992230 290 + 0.557 0.980 1.716
ENSG00000127415 E013 0.2965864 0.461481265 3.742871e-01   4 999710 999741 32 + 0.000 0.189 11.848
ENSG00000127415 E014 2.0284229 0.039076017 5.958225e-01 7.178602e-01 4 999742 1000064 323 + 0.396 0.480 0.438
ENSG00000127415 E015 11.6500448 0.001643086 8.865685e-01 9.314633e-01 4 1000612 1000697 86 + 1.085 1.073 -0.043
ENSG00000127415 E016 17.2614879 0.001088779 3.914687e-02 8.945596e-02 4 1000882 1000989 108 + 1.147 1.291 0.509
ENSG00000127415 E017 6.2740732 0.002914061 2.249782e-01 3.563904e-01 4 1000990 1001179 190 + 0.766 0.894 0.499
ENSG00000127415 E018 15.3211191 0.001736478 8.256981e-01 8.902609e-01 4 1001468 1001563 96 + 1.190 1.184 -0.024
ENSG00000127415 E019 16.8074898 0.001141337 9.130143e-01 9.488936e-01 4 1001679 1001881 203 + 1.240 1.222 -0.062
ENSG00000127415 E020 2.8506457 0.085184475 6.224049e-01 7.392184e-01 4 1001882 1001981 100 + 0.639 0.519 -0.544
ENSG00000127415 E021 14.0878863 0.001431047 4.559368e-01 5.964196e-01 4 1001982 1002161 180 + 1.135 1.175 0.143
ENSG00000127415 E022 3.6911768 0.004573676 1.302352e-01 2.344932e-01 4 1002162 1002268 107 + 0.793 0.552 -1.023
ENSG00000127415 E023 15.1044579 0.002871257 5.815377e-01 7.060677e-01 4 1002269 1002485 217 + 1.169 1.192 0.081
ENSG00000127415 E024 3.4644159 0.005117639 3.684923e-01 5.129003e-01 4 1002732 1002740 9 + 0.557 0.673 0.509
ENSG00000127415 E025 10.1996332 0.033586692 2.184791e-01 3.485756e-01 4 1002741 1002944 204 + 1.123 0.947 -0.645
ENSG00000127415 E026 6.4666443 0.057450987 1.640570e-02 4.362635e-02 4 1003036 1003157 122 + 1.044 0.654 -1.518
ENSG00000127415 E027 2.7980937 0.025650434 1.233669e-01 2.247582e-01 4 1003158 1003344 187 + 0.707 0.434 -1.253
ENSG00000127415 E028 4.7366533 0.154208698 4.335338e-01 5.759504e-01 4 1003345 1003412 68 + 0.818 0.685 -0.538
ENSG00000127415 E029 3.4456294 0.224879479 8.456702e-01 9.040260e-01 4 1003413 1003428 16 + 0.638 0.629 -0.040
ENSG00000127415 E030 5.5188791 0.003169965 6.869935e-01 7.894095e-01 4 1003429 1003470 42 + 0.842 0.767 -0.297
ENSG00000127415 E031 12.0227057 0.010433174 9.841854e-01 9.942040e-01 4 1003549 1003625 77 + 1.123 1.093 -0.109
ENSG00000127415 E032 1.3422170 0.577584071 5.751350e-01 7.008362e-01 4 1003626 1003629 4 + 0.458 0.313 -0.826
ENSG00000127415 E033 2.3260454 0.006600209 2.771569e-02 6.745358e-02 4 1003630 1003724 95 + 0.707 0.330 -1.845
ENSG00000127415 E034 19.8827732 0.019442141 1.964504e-01 3.217872e-01 4 1004012 1004112 101 + 1.249 1.344 0.333
ENSG00000127415 E035 22.2036297 0.011419336 1.066220e-01 2.005682e-01 4 1004260 1004564 305 + 1.284 1.392 0.378