ENSG00000127314

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000250559 ENSG00000127314 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1B protein_coding protein_coding 62.89587 25.83393 107.0708 1.163125 0.6818503 2.050803 2.498217 2.369184 3.417836 0.4213331 0.6528139 0.5268313 0.05556667 0.09223333 0.03186667 -0.06036667 0.009434604 1.143002e-08 FALSE TRUE
ENST00000341355 ENSG00000127314 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1B protein_coding protein_coding 62.89587 25.83393 107.0708 1.163125 0.6818503 2.050803 39.074829 18.250011 69.481727 2.2467217 1.7106666 1.9281536 0.66466250 0.70133333 0.64880000 -0.05253333 0.698122700 1.143002e-08 FALSE TRUE
ENST00000535492 ENSG00000127314 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1B protein_coding protein_coding 62.89587 25.83393 107.0708 1.163125 0.6818503 2.050803 5.074307 1.443596 11.504639 0.7339542 0.8319244 2.9857706 0.06531250 0.05666667 0.10736667 0.05070000 0.675965176 1.143002e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000127314 E001 0.7363589 0.0155083874 2.081211e-01 3.362148e-01 12 68610855 68610857 3 + 0.273 0.000 -10.708
ENSG00000127314 E002 6.7256483 0.0026104939 3.615816e-02 8.389431e-02 12 68610858 68610898 41 + 0.894 0.605 -1.176
ENSG00000127314 E003 9.7891985 0.0017447449 2.275559e-02 5.724311e-02 12 68610899 68610907 9 + 1.034 0.766 -1.020
ENSG00000127314 E004 12.2429131 0.0015230080 2.473697e-02 6.133477e-02 12 68610908 68610912 5 + 1.117 0.884 -0.862
ENSG00000127314 E005 21.1449028 0.0027395999 8.129727e-03 2.411273e-02 12 68610913 68610917 5 + 1.336 1.117 -0.773
ENSG00000127314 E006 23.6813489 0.0007929288 1.399460e-03 5.305018e-03 12 68610918 68610918 1 + 1.389 1.137 -0.887
ENSG00000127314 E007 52.5566247 0.0014079688 2.485889e-07 2.339747e-06 12 68610919 68610924 6 + 1.733 1.431 -1.030
ENSG00000127314 E008 61.2348167 0.0004305731 9.686957e-08 9.860646e-07 12 68610925 68610928 4 + 1.793 1.519 -0.932
ENSG00000127314 E009 70.9617503 0.0005716082 1.700310e-04 8.351965e-04 12 68610929 68610932 4 + 1.836 1.677 -0.539
ENSG00000127314 E010 87.2759228 0.0005487660 2.365224e-04 1.118701e-03 12 68610933 68610937 5 + 1.920 1.784 -0.457
ENSG00000127314 E011 87.8566135 0.0006586979 1.875554e-04 9.104182e-04 12 68610938 68610938 1 + 1.924 1.784 -0.469
ENSG00000127314 E012 124.1631123 0.0008384661 3.111416e-03 1.059397e-02 12 68610939 68610960 22 + 2.061 1.979 -0.274
ENSG00000127314 E013 181.6369732 0.0002197762 5.611396e-05 3.122612e-04 12 68610961 68611024 64 + 2.226 2.135 -0.304
ENSG00000127314 E014 126.5606791 0.0047092930 5.522365e-02 1.183760e-01 12 68611025 68611028 4 + 2.062 2.000 -0.210
ENSG00000127314 E015 143.7248693 0.0002939037 2.476504e-03 8.693255e-03 12 68611029 68611035 7 + 2.119 2.048 -0.238
ENSG00000127314 E016 149.3375264 0.0002358213 5.310373e-04 2.275735e-03 12 68611036 68611043 8 + 2.139 2.055 -0.281
ENSG00000127314 E017 45.7151376 0.0005049318 2.122732e-01 3.412116e-01 12 68611044 68611052 9 + 1.625 1.585 -0.136
ENSG00000127314 E018 0.4502799 0.0291162999 4.676169e-01 6.071166e-01 12 68611053 68611056 4 + 0.184 0.000 -11.802
ENSG00000127314 E019 0.4386386 0.1763969986 7.114843e-01 8.084322e-01 12 68621446 68621491 46 + 0.130 0.204 0.775
ENSG00000127314 E020 0.8126314 0.0139165892 8.502161e-01 9.070985e-01 12 68624508 68624670 163 + 0.231 0.205 -0.212
ENSG00000127314 E021 4.4414557 0.0037033772 7.568722e-01 8.418884e-01 12 68624671 68624674 4 + 0.686 0.666 -0.087
ENSG00000127314 E022 10.6433611 0.0163455234 8.561602e-02 1.683834e-01 12 68624675 68624753 79 + 1.055 0.849 -0.772
ENSG00000127314 E023 10.2381377 0.0136091539 8.715859e-04 3.514748e-03 12 68624754 68624873 120 + 1.081 0.604 -1.870
ENSG00000127314 E024 0.0000000       12 68641034 68641037 4 +      
ENSG00000127314 E025 1.0435544 0.0198764814 7.884474e-01 8.645826e-01 12 68641038 68641056 19 + 0.273 0.344 0.461
ENSG00000127314 E026 3.1451452 0.0337107336 3.110851e-01 4.535304e-01 12 68641057 68641153 97 + 0.516 0.718 0.890
ENSG00000127314 E027 0.4417591 0.0740842532 1.344614e-01 2.403647e-01 12 68647993 68648095 103 + 0.070 0.342 2.775
ENSG00000127314 E028 0.2944980 0.3622070065 1.000000e+00   12 68648670 68648698 29 + 0.130 0.000 -10.304
ENSG00000127314 E029 135.3820804 0.0002208467 3.605254e-04 1.620914e-03 12 68648699 68648705 7 + 2.099 2.006 -0.314
ENSG00000127314 E030 269.0905247 0.0001579213 4.288617e-01 5.715913e-01 12 68648706 68648781 76 + 2.370 2.386 0.055
ENSG00000127314 E031 2.3713149 0.0397342615 8.649194e-03 2.540685e-02 12 68648782 68650041 1260 + 0.580 0.000 -14.077
ENSG00000127314 E032 1.3338852 0.0100282473 3.984702e-01 5.422206e-01 12 68650244 68650399 156 + 0.273 0.449 1.051
ENSG00000127314 E033 305.9941340 0.0002207556 7.254244e-01 8.187003e-01 12 68650400 68650468 69 + 2.419 2.463 0.147
ENSG00000127314 E034 0.3030308 0.3828854368 1.000000e+00   12 68650469 68650882 414 + 0.131 0.000 -10.291
ENSG00000127314 E035 0.4449813 0.0214518449 7.017726e-01 8.007879e-01 12 68651682 68651994 313 + 0.130 0.205 0.785
ENSG00000127314 E036 224.7126608 0.0001777463 8.934583e-01 9.358972e-01 12 68651995 68651998 4 + 2.286 2.325 0.132
ENSG00000127314 E037 264.4979836 0.0001683154 6.785366e-01 7.829857e-01 12 68651999 68652018 20 + 2.354 2.400 0.154
ENSG00000127314 E038 317.3282112 0.0001428109 9.290054e-01 9.593590e-01 12 68652019 68652051 33 + 2.435 2.473 0.126
ENSG00000127314 E039 295.9916099 0.0001770256 7.300210e-01 8.220710e-01 12 68654112 68654124 13 + 2.408 2.435 0.093
ENSG00000127314 E040 411.8739706 0.0001382433 1.898712e-01 3.135944e-01 12 68654125 68654252 128 + 2.555 2.565 0.033
ENSG00000127314 E041 251.8195673 0.0001740590 1.483859e-01 2.594822e-01 12 68656306 68656449 144 + 2.325 2.397 0.240
ENSG00000127314 E042 0.1482932 0.0412044700 1.440218e-01   12 68656450 68656637 188 + 0.000 0.205 11.783
ENSG00000127314 E043 202.2991982 0.0002105029 6.587387e-05 3.602242e-04 12 68657101 68657217 117 + 2.209 2.354 0.483
ENSG00000127314 E044 2.0014602 0.0068764227 1.271587e-01 2.301724e-01 12 68657218 68657713 496 + 0.492 0.205 -1.798
ENSG00000127314 E045 498.2167032 0.0099860228 2.361303e-10 3.905551e-09 12 68659280 68671901 12622 + 2.528 2.862 1.110