ENSG00000126953

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372902 ENSG00000126953 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM8A protein_coding protein_coding 38.05508 56.14228 32.68369 5.633045 0.3134646 -0.7803325 24.876049 33.075800 24.0826482 4.8785612 0.4853035 -0.4576192 0.67575417 0.5841333 0.736700000 0.1525667 0.001410081 0.001410081 FALSE FALSE
MSTRG.34597.7 ENSG00000126953 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM8A protein_coding   38.05508 56.14228 32.68369 5.633045 0.3134646 -0.7803325 7.270951 11.909130 5.8202359 1.6431624 0.2096208 -1.0316528 0.17761667 0.2130333 0.178233333 -0.0348000 0.619064388 0.001410081 FALSE FALSE
MSTRG.34597.8 ENSG00000126953 HEK293_OSMI2_2hA HEK293_TMG_2hB TIMM8A protein_coding   38.05508 56.14228 32.68369 5.633045 0.3134646 -0.7803325 3.846053 9.062249 0.1784007 0.5385569 0.1784007 -5.5895842 0.09067083 0.1653667 0.005366667 -0.1600000 0.003954119 0.001410081   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000126953 E001 64.043424 0.0009249264 1.426436e-21 1.085277e-19 X 101345661 101345783 123 - 2.014 1.615 -1.344
ENSG00000126953 E002 132.283191 0.0008242231 1.031375e-41 3.995724e-39 X 101345784 101346091 308 - 2.331 1.927 -1.351
ENSG00000126953 E003 132.051053 0.0003190073 1.235381e-41 4.756896e-39 X 101346092 101346199 108 - 2.319 1.940 -1.269
ENSG00000126953 E004 122.110111 0.0024194892 2.835598e-21 2.094330e-19 X 101346200 101346252 53 - 2.263 1.928 -1.121
ENSG00000126953 E005 767.504092 0.0002708136 4.305863e-04 1.892125e-03 X 101346253 101346660 408 - 2.819 2.890 0.235
ENSG00000126953 E006 48.578276 0.0020064267 1.732618e-03 6.385480e-03 X 101346661 101347926 1266 - 1.768 1.630 -0.469
ENSG00000126953 E007 12.880712 0.0016872322 2.288991e-06 1.757559e-05 X 101347927 101348040 114 - 1.343 0.932 -1.476
ENSG00000126953 E008 12.289176 0.0368352534 3.615891e-01 5.059828e-01 X 101348041 101348060 20 - 1.160 1.058 -0.370
ENSG00000126953 E009 101.858681 0.0088012915 1.401640e-09 2.022519e-08 X 101348061 101348401 341 - 1.693 2.082 1.307
ENSG00000126953 E010 4.654198 0.0105515837 4.939394e-01 6.309697e-01 X 101348402 101348532 131 - 0.640 0.763 0.510
ENSG00000126953 E011 406.955074 0.0002172877 6.854332e-31 1.226187e-28 X 101348533 101348592 60 - 2.414 2.661 0.822
ENSG00000126953 E012 414.847849 0.0055049554 7.375014e-09 9.311391e-08 X 101348593 101348742 150 - 2.432 2.658 0.752