ENSG00000126778

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000554986 ENSG00000126778 HEK293_OSMI2_2hA HEK293_TMG_2hB SIX1 protein_coding protein_coding 17.85429 15.1266 19.78442 1.367818 0.1647182 0.3870525 4.626002 5.2903104 3.673091 0.6939476 0.3173548 -0.5251599 0.26042083 0.34726667 0.1857667 -0.161500000 0.0001768348 0.0001768348 FALSE TRUE
ENST00000645694 ENSG00000126778 HEK293_OSMI2_2hA HEK293_TMG_2hB SIX1 protein_coding protein_coding 17.85429 15.1266 19.78442 1.367818 0.1647182 0.3870525 1.086392 0.8300472 1.264868 0.1762886 0.2059712 0.6018051 0.05965417 0.05383333 0.0638000 0.009966667 0.8298893291 0.0001768348 FALSE TRUE
MSTRG.9653.2 ENSG00000126778 HEK293_OSMI2_2hA HEK293_TMG_2hB SIX1 protein_coding   17.85429 15.1266 19.78442 1.367818 0.1647182 0.3870525 10.081972 7.6715652 11.631418 0.5442215 0.5462367 0.5997946 0.56983750 0.51120000 0.5884333 0.077233333 0.4936602999 0.0001768348 TRUE TRUE
MSTRG.9653.6 ENSG00000126778 HEK293_OSMI2_2hA HEK293_TMG_2hB SIX1 protein_coding   17.85429 15.1266 19.78442 1.367818 0.1647182 0.3870525 2.009563 1.3291225 3.176389 0.4001857 0.6472619 1.2506347 0.10751667 0.08736667 0.1600667 0.072700000 0.3466594009 0.0001768348 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000126778 E001 4.3273272 0.0037552375 4.076919e-01 5.512723e-01 14 60643421 60643510 90 - 0.786 0.688 -0.400
ENSG00000126778 E002 7.7947658 0.0028430837 8.645360e-01 9.166779e-01 14 60643511 60643619 109 - 0.960 0.959 -0.004
ENSG00000126778 E003 29.2921916 0.0148338626 8.950648e-01 9.369462e-01 14 60643620 60644695 1076 - 1.483 1.494 0.039
ENSG00000126778 E004 50.9915965 0.0004999050 5.767863e-03 1.800320e-02 14 60644696 60644930 235 - 1.637 1.789 0.516
ENSG00000126778 E005 86.8549873 0.0003428419 2.805971e-05 1.680310e-04 14 60644931 60645245 315 - 1.851 2.025 0.584
ENSG00000126778 E006 29.9652948 0.0006947029 2.808753e-01 4.207489e-01 14 60645246 60645269 24 - 1.448 1.533 0.293
ENSG00000126778 E007 405.1082182 0.0029567266 8.074785e-06 5.477652e-05 14 60645270 60646156 887 - 2.546 2.666 0.401
ENSG00000126778 E008 251.9054871 0.0002420995 2.347725e-04 1.111757e-03 14 60646157 60646577 421 - 2.434 2.376 -0.192
ENSG00000126778 E009 84.1768200 0.0012739265 8.168340e-05 4.359677e-04 14 60648630 60648688 59 - 1.998 1.856 -0.478
ENSG00000126778 E010 288.2539374 0.0008150255 1.712570e-07 1.662949e-06 14 60648689 60649477 789 - 2.509 2.414 -0.316
ENSG00000126778 E011 0.0000000       14 60651775 60651934 160 -      
ENSG00000126778 E012 0.1515154 0.0422965515 5.549449e-01   14 60651935 60652118 184 - 0.114 0.000 -9.110
ENSG00000126778 E013 0.4407149 0.0228673107 6.403229e-01 7.535927e-01 14 60652119 60652962 844 - 0.204 0.131 -0.772
ENSG00000126778 E014 0.3268771 0.0287503255 9.320016e-01   14 60652963 60653131 169 - 0.114 0.131 0.231
ENSG00000126778 E015 0.8083901 0.0161777011 5.670546e-01 6.939291e-01 14 60658183 60658259 77 - 0.204 0.313 0.814