ENSG00000126775

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000247178 ENSG00000126775 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG14 protein_coding protein_coding 3.613496 1.547883 5.616571 0.2622789 0.03208866 1.852669 3.1336161 1.51118976 4.9734275 0.28241200 0.1220511 1.711938 0.867325 0.97146667 0.8854 -0.08606667 0.04553016 0.04553016 FALSE  
ENST00000558189 ENSG00000126775 HEK293_OSMI2_2hA HEK293_TMG_2hB ATG14 protein_coding retained_intron 3.613496 1.547883 5.616571 0.2622789 0.03208866 1.852669 0.4798801 0.03669313 0.6431432 0.03669313 0.1062890 3.806117 0.132675 0.02853333 0.1146 0.08606667 0.04660374 0.04553016 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000126775 E001 0.1482932 0.0411597534 1.329905e-01   14 55366391 55366391 1 - 0.000 0.206 10.288
ENSG00000126775 E002 315.6832873 0.0014543826 7.714276e-10 1.168409e-08 14 55366392 55369925 3534 - 2.414 2.529 0.384
ENSG00000126775 E003 25.7786729 0.0008325147 2.503524e-01 3.863578e-01 14 55377819 55377904 86 - 1.392 1.324 -0.237
ENSG00000126775 E004 19.4334513 0.0010727660 1.504941e-01 2.623594e-01 14 55377984 55378074 91 - 1.285 1.176 -0.384
ENSG00000126775 E005 21.8167331 0.0010449731 1.626860e-02 4.331808e-02 14 55380573 55380690 118 - 1.350 1.158 -0.677
ENSG00000126775 E006 31.5735401 0.0007130768 2.419577e-03 8.521054e-03 14 55381962 55382191 230 - 1.505 1.298 -0.715
ENSG00000126775 E007 36.4597998 0.0005694776 2.940687e-02 7.078475e-02 14 55385859 55386096 238 - 1.550 1.424 -0.430
ENSG00000126775 E008 26.1495343 0.0057643492 4.726078e-02 1.042987e-01 14 55390911 55390992 82 - 1.413 1.256 -0.546
ENSG00000126775 E009 4.1856714 0.0039819361 3.846087e-03 1.271028e-02 14 55390993 55391302 310 - 0.756 0.207 -2.948
ENSG00000126775 E010 19.4598923 0.0207537110 5.849878e-01 7.087758e-01 14 55395940 55395982 43 - 1.235 1.300 0.229
ENSG00000126775 E011 19.9211146 0.0009630623 3.029023e-01 4.448260e-01 14 55397372 55397434 63 - 1.281 1.210 -0.249
ENSG00000126775 E012 18.1275775 0.0010825657 7.767422e-03 2.319470e-02 14 55411602 55411830 229 - 1.277 1.031 -0.879