ENSG00000126453

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000246784 ENSG00000126453 HEK293_OSMI2_2hA HEK293_TMG_2hB BCL2L12 protein_coding protein_coding 140.3097 258.4115 68.03449 16.21447 2.270325 -1.925176 83.814463 164.657720 35.034124 11.9811514 1.8915044 -2.2323133 0.56767917 0.63636667 0.51443333 -0.12193333 4.197927e-05 1.353299e-06 FALSE TRUE
ENST00000594157 ENSG00000126453 HEK293_OSMI2_2hA HEK293_TMG_2hB BCL2L12 protein_coding protein_coding 140.3097 258.4115 68.03449 16.21447 2.270325 -1.925176 11.650610 18.857715 6.494386 2.7510267 0.9530854 -1.5364350 0.09278750 0.07310000 0.09486667 0.02176667 5.369321e-01 1.353299e-06 FALSE FALSE
ENST00000598979 ENSG00000126453 HEK293_OSMI2_2hA HEK293_TMG_2hB BCL2L12 protein_coding nonsense_mediated_decay 140.3097 258.4115 68.03449 16.21447 2.270325 -1.925176 15.050040 28.549579 5.451895 2.1714138 1.5499121 -2.3865012 0.10023333 0.11070000 0.08106667 -0.02963333 5.846754e-01 1.353299e-06 TRUE FALSE
ENST00000601168 ENSG00000126453 HEK293_OSMI2_2hA HEK293_TMG_2hB BCL2L12 protein_coding retained_intron 140.3097 258.4115 68.03449 16.21447 2.270325 -1.925176 6.764826 6.251536 5.785005 0.6873595 1.1014310 -0.1117068 0.06277083 0.02403333 0.08476667 0.06073333 2.069622e-05 1.353299e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000126453 E001 3.610737 4.503385e-03 1.627748e-05 1.030406e-04 19 49665142 49665565 424 + 1.007 0.371 -2.763
ENSG00000126453 E002 7.925557 2.131461e-02 8.928680e-03 2.610055e-02 19 49665566 49665932 367 + 1.090 0.733 -1.359
ENSG00000126453 E003 34.443388 5.566888e-03 3.086127e-01 4.508915e-01 19 49665933 49665960 28 + 1.466 1.385 -0.277
ENSG00000126453 E004 93.415706 2.954464e-03 6.933422e-01 7.943852e-01 19 49665961 49665997 37 + 1.837 1.821 -0.055
ENSG00000126453 E005 92.643848 2.563368e-03 8.512731e-01 9.077820e-01 19 49665998 49665998 1 + 1.824 1.818 -0.017
ENSG00000126453 E006 203.542832 1.523487e-03 3.816148e-01 5.259230e-01 19 49665999 49666012 14 + 2.126 2.165 0.132
ENSG00000126453 E007 869.440420 1.489675e-04 1.712431e-01 2.898089e-01 19 49666013 49666067 55 + 2.765 2.792 0.089
ENSG00000126453 E008 1408.479445 5.133779e-04 8.240975e-01 8.891833e-01 19 49666685 49666799 115 + 2.998 3.000 0.008
ENSG00000126453 E009 56.243145 7.193093e-03 2.462705e-15 8.852982e-14 19 49666800 49667018 219 + 1.993 1.490 -1.703
ENSG00000126453 E010 615.050950 9.024707e-04 2.921631e-01 4.331076e-01 19 49667019 49667021 3 + 2.656 2.638 -0.061
ENSG00000126453 E011 1082.396308 9.037505e-04 4.621698e-01 6.020455e-01 19 49667022 49667087 66 + 2.869 2.890 0.069
ENSG00000126453 E012 1031.796047 9.133045e-05 2.514717e-01 3.877188e-01 19 49667088 49667161 74 + 2.845 2.866 0.069
ENSG00000126453 E013 1320.058699 8.005312e-05 4.158125e-01 5.591518e-01 19 49668851 49668937 87 + 2.975 2.968 -0.023
ENSG00000126453 E014 771.543281 1.159102e-04 9.188541e-02 1.780500e-01 19 49669024 49669047 24 + 2.758 2.733 -0.084
ENSG00000126453 E015 1072.682178 1.249155e-04 3.749242e-01 5.193567e-01 19 49669048 49669115 68 + 2.888 2.879 -0.030
ENSG00000126453 E016 27.312234 7.340893e-04 4.873212e-09 6.372276e-08 19 49669116 49669164 49 + 1.637 1.212 -1.467
ENSG00000126453 E017 1246.554766 1.953356e-03 1.303933e-02 3.600283e-02 19 49670216 49670436 221 + 2.891 2.957 0.222
ENSG00000126453 E018 533.064156 2.048592e-03 1.723331e-02 4.546187e-02 19 49670437 49670488 52 + 2.512 2.590 0.259
ENSG00000126453 E019 37.452396 5.390212e-04 2.614509e-08 2.979135e-07 19 49672109 49672201 93 + 1.720 1.363 -1.223
ENSG00000126453 E020 884.855222 6.787833e-04 9.053314e-01 9.437184e-01 19 49673698 49673916 219 + 2.796 2.799 0.010