ENSG00000125977

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374980 ENSG00000125977 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S2 protein_coding protein_coding 152.5722 88.98633 211.5287 3.036996 11.22251 1.249104 17.07577 14.86553 20.73992 1.544803 0.4023576 0.4801644 0.1271625 0.1667000 0.09856667 -0.068133333 0.001492484 0.001492484 FALSE  
MSTRG.20583.10 ENSG00000125977 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S2 protein_coding   152.5722 88.98633 211.5287 3.036996 11.22251 1.249104 50.16981 17.47200 78.19462 3.812310 9.6588103 2.1613834 0.2974458 0.1975333 0.36680000 0.169266667 0.079023675 0.001492484 FALSE  
MSTRG.20583.6 ENSG00000125977 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S2 protein_coding   152.5722 88.98633 211.5287 3.036996 11.22251 1.249104 59.86366 36.50638 87.74457 1.532596 4.2612000 1.2649306 0.3895333 0.4100333 0.41546667 0.005433333 0.943809932 0.001492484 FALSE  
MSTRG.20583.8 ENSG00000125977 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S2 protein_coding   152.5722 88.98633 211.5287 3.036996 11.22251 1.249104 20.35542 16.92633 18.62377 2.480092 4.1411236 0.1377977 0.1519833 0.1896000 0.08956667 -0.100033333 0.096428526 0.001492484 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125977 E001 0.0000000       20 34087933 34088124 192 -      
ENSG00000125977 E002 0.1472490 4.469264e-02 8.731210e-01   20 34088125 34088308 184 - 0.092 0.000 -9.066
ENSG00000125977 E003 20.2434868 5.089741e-03 4.632042e-05 2.631185e-04 20 34088309 34088397 89 - 1.155 1.507 1.229
ENSG00000125977 E004 472.3281000 3.794518e-03 9.110640e-07 7.628993e-06 20 34088398 34089515 1118 - 2.585 2.769 0.613
ENSG00000125977 E005 1624.9349243 2.691672e-03 7.820273e-07 6.646004e-06 20 34089516 34089905 390 - 3.146 3.276 0.432
ENSG00000125977 E006 696.1933892 8.663287e-04 7.322209e-02 1.486753e-01 20 34090517 34090602 86 - 2.813 2.867 0.177
ENSG00000125977 E007 0.0000000       20 34091777 34091778 2 -      
ENSG00000125977 E008 0.6653823 1.726718e-02 6.588853e-01 7.678679e-01 20 34091779 34091796 18 - 0.233 0.161 -0.669
ENSG00000125977 E009 2.2582886 1.720182e-02 1.952084e-01 3.202467e-01 20 34091797 34091824 28 - 0.384 0.617 1.141
ENSG00000125977 E010 2.4797338 6.759596e-02 1.496996e-01 2.612724e-01 20 34091825 34091827 3 - 0.384 0.665 1.350
ENSG00000125977 E011 12.1929577 1.719624e-03 2.431687e-03 8.557499e-03 20 34091828 34091913 86 - 0.957 1.266 1.113
ENSG00000125977 E012 607.8297738 1.472277e-04 5.902573e-03 1.835322e-02 20 34093675 34093731 57 - 2.750 2.810 0.202
ENSG00000125977 E013 1000.1993975 8.601104e-05 4.388698e-02 9.817629e-02 20 34096657 34096805 149 - 2.992 2.989 -0.013
ENSG00000125977 E014 963.7607189 2.490603e-04 3.449075e-12 7.752074e-11 20 34097416 34097516 101 - 3.001 2.928 -0.243
ENSG00000125977 E015 838.1006971 1.307391e-03 2.644046e-03 9.203105e-03 20 34098498 34098633 136 - 2.930 2.885 -0.151
ENSG00000125977 E016 5.6602699 2.922215e-03 5.200824e-01 6.541092e-01 20 34103439 34103461 23 - 0.841 0.772 -0.270
ENSG00000125977 E017 337.1985241 3.856109e-03 2.054664e-01 3.329882e-01 20 34103462 34103497 36 - 2.531 2.498 -0.111
ENSG00000125977 E018 400.1642199 2.480908e-03 7.937598e-02 1.585319e-01 20 34103498 34103565 68 - 2.607 2.569 -0.128
ENSG00000125977 E019 864.8286890 1.837147e-03 3.955236e-05 2.282387e-04 20 34105368 34105545 178 - 2.956 2.877 -0.264
ENSG00000125977 E020 2.7377147 5.619907e-03 9.717461e-01 9.863556e-01 20 34106214 34106289 76 - 0.557 0.566 0.040
ENSG00000125977 E021 0.0000000       20 34106877 34106926 50 -      
ENSG00000125977 E022 1.1823723 1.149009e-02 1.589755e-02 4.250754e-02 20 34111680 34111740 61 - 0.462 0.000 -12.062
ENSG00000125977 E023 434.4947489 2.185139e-03 5.466490e-06 3.857308e-05 20 34112096 34112296 201 - 2.669 2.558 -0.371