ENSG00000125826

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000356286 ENSG00000125826 HEK293_OSMI2_2hA HEK293_TMG_2hB RBCK1 protein_coding protein_coding 41.80347 66.87471 22.88053 2.225104 0.348917 -1.546925 5.949533 9.319545 4.0223092 0.8292753 0.2203364 -1.2102005 0.14458333 0.13890000 0.1761667 0.03726667 2.556721e-01 2.856914e-19 FALSE TRUE
ENST00000400245 ENSG00000125826 HEK293_OSMI2_2hA HEK293_TMG_2hB RBCK1 protein_coding processed_transcript 41.80347 66.87471 22.88053 2.225104 0.348917 -1.546925 2.182368 3.403267 0.6844552 0.1576875 0.3799934 -2.2971999 0.05180833 0.05113333 0.0295000 -0.02163333 4.460381e-01 2.856914e-19 FALSE TRUE
ENST00000400247 ENSG00000125826 HEK293_OSMI2_2hA HEK293_TMG_2hB RBCK1 protein_coding protein_coding 41.80347 66.87471 22.88053 2.225104 0.348917 -1.546925 4.239971 8.463424 2.3249451 0.3973464 0.2147460 -1.8595564 0.09482083 0.12716667 0.1015000 -0.02566667 4.347556e-01 2.856914e-19 TRUE FALSE
ENST00000475269 ENSG00000125826 HEK293_OSMI2_2hA HEK293_TMG_2hB RBCK1 protein_coding protein_coding 41.80347 66.87471 22.88053 2.225104 0.348917 -1.546925 13.130017 23.636285 5.3813275 4.7010508 0.2768082 -2.1329011 0.29110000 0.35100000 0.2352333 -0.11576667 3.646226e-01 2.856914e-19 FALSE TRUE
ENST00000621487 ENSG00000125826 HEK293_OSMI2_2hA HEK293_TMG_2hB RBCK1 protein_coding retained_intron 41.80347 66.87471 22.88053 2.225104 0.348917 -1.546925 4.926608 3.148788 4.3928143 0.2900679 0.2638643 0.4790547 0.14256667 0.04693333 0.1922000 0.14526667 2.856914e-19 2.856914e-19   FALSE
MSTRG.20225.4 ENSG00000125826 HEK293_OSMI2_2hA HEK293_TMG_2hB RBCK1 protein_coding   41.80347 66.87471 22.88053 2.225104 0.348917 -1.546925 5.421268 9.879551 2.3967374 3.7118788 0.4245844 -2.0388262 0.12735000 0.14966667 0.1053000 -0.04436667 8.280462e-01 2.856914e-19 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125826 E001 2.0626762 0.0084262678 1.860291e-01 3.087745e-01 20 407498 407539 42 + 0.201 0.474 1.747
ENSG00000125826 E002 1.5112334 0.0236690225 3.766473e-01 5.209665e-01 20 407540 407621 82 + 0.201 0.386 1.284
ENSG00000125826 E003 4.1620730 0.0041119653 2.331799e-01 3.662014e-01 20 407622 407671 50 + 0.442 0.663 1.027
ENSG00000125826 E004 0.5117019 0.0270274040 4.913289e-01 6.286099e-01 20 408050 408052 3 + 0.000 0.187 9.301
ENSG00000125826 E005 8.4108700 0.0404551149 9.269574e-01 9.580327e-01 20 408053 408064 12 + 0.874 0.893 0.072
ENSG00000125826 E006 12.7492641 0.0029718768 9.587461e-01 9.781493e-01 20 408065 408077 13 + 1.042 1.058 0.058
ENSG00000125826 E007 46.0398049 0.0067108973 6.027947e-04 2.543448e-03 20 408078 408146 69 + 1.323 1.618 1.015
ENSG00000125826 E008 47.9907050 0.0067965214 7.041258e-05 3.820988e-04 20 408147 408170 24 + 1.298 1.640 1.179
ENSG00000125826 E009 97.2328574 0.0024162975 1.976969e-06 1.539505e-05 20 408171 408298 128 + 1.660 1.933 0.921
ENSG00000125826 E010 59.2812342 0.0007689955 1.203315e-03 4.651862e-03 20 408299 408304 6 + 1.500 1.711 0.720
ENSG00000125826 E011 60.6066102 0.0023971711 1.747492e-03 6.432541e-03 20 408305 408312 8 + 1.508 1.722 0.729
ENSG00000125826 E012 97.9311594 0.0083531100 6.909117e-02 1.418713e-01 20 408313 408342 30 + 1.786 1.919 0.449
ENSG00000125826 E013 262.7136787 0.0020698683 1.589045e-01 2.736824e-01 20 408343 408511 169 + 2.271 2.333 0.208
ENSG00000125826 E014 371.6969122 0.0001524204 3.032357e-01 4.451331e-01 20 408512 408690 179 + 2.443 2.477 0.114
ENSG00000125826 E015 276.3646377 0.0001616909 6.889768e-01 7.909660e-01 20 408691 408779 89 + 2.326 2.347 0.072
ENSG00000125826 E016 12.4127072 0.0060422616 3.118486e-01 4.543717e-01 20 409830 409880 51 + 0.937 1.070 0.491
ENSG00000125826 E017 127.7833506 0.0003207995 5.263628e-01 6.595968e-01 20 409881 409883 3 + 1.984 2.020 0.119
ENSG00000125826 E018 334.8346604 0.0005904749 3.214552e-01 4.647055e-01 20 409884 410025 142 + 2.398 2.435 0.124
ENSG00000125826 E019 356.6372867 0.0065371112 3.271956e-05 1.928992e-04 20 410412 410764 353 + 2.291 2.492 0.671
ENSG00000125826 E020 16.7198406 0.0084281609 4.487091e-01 5.899876e-01 20 417220 417342 123 + 1.086 1.181 0.340
ENSG00000125826 E021 122.8800433 0.0041706451 1.465649e-04 7.323104e-04 20 417526 417619 94 + 2.118 1.955 -0.546
ENSG00000125826 E022 156.5411507 0.0009793044 1.913125e-07 1.840242e-06 20 417732 417930 199 + 2.223 2.056 -0.559
ENSG00000125826 E023 108.9530845 0.0006661778 3.115948e-04 1.426896e-03 20 419347 419468 122 + 2.041 1.909 -0.445
ENSG00000125826 E024 128.4577932 0.0011361002 8.524928e-03 2.509981e-02 20 419558 419731 174 + 2.081 1.990 -0.304
ENSG00000125826 E025 91.7603181 0.0063208850 9.437246e-01 9.686954e-01 20 420871 421006 136 + 1.855 1.869 0.045
ENSG00000125826 E026 53.3687662 0.0017824707 5.728918e-01 6.989255e-01 20 421007 421031 25 + 1.649 1.627 -0.076
ENSG00000125826 E027 21.3221269 0.0009693356 6.300595e-14 1.841451e-12 20 421942 422126 185 + 1.626 1.072 -1.933
ENSG00000125826 E028 81.8940945 0.0023889534 3.333279e-02 7.844857e-02 20 422127 422153 27 + 1.890 1.795 -0.319
ENSG00000125826 E029 146.6441935 0.0002271367 8.630072e-04 3.484158e-03 20 422154 422238 85 + 2.148 2.047 -0.339
ENSG00000125826 E030 233.0151706 0.0004812119 1.947181e-06 1.518371e-05 20 427313 427492 180 + 2.362 2.242 -0.401
ENSG00000125826 E031 188.3120381 0.0002287246 3.909046e-02 8.935615e-02 20 428491 428589 99 + 2.218 2.167 -0.173
ENSG00000125826 E032 16.9800044 0.0012112054 8.532245e-12 1.797607e-10 20 428590 428950 361 + 1.539 0.984 -1.960
ENSG00000125826 E033 180.9950339 0.0002794255 1.591643e-01 2.740004e-01 20 428951 429094 144 + 2.116 2.173 0.191
ENSG00000125826 E034 469.2420849 0.0051452793 6.331050e-01 7.478535e-01 20 430350 432139 1790 + 2.551 2.584 0.109