ENSG00000125814

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377026 ENSG00000125814 HEK293_OSMI2_2hA HEK293_TMG_2hB NAPB protein_coding protein_coding 4.161378 2.128759 5.552327 0.4410373 0.3128725 1.378914 0.87232068 0.05951800 0.6980955 0.05951800 0.4556375 3.3484855 0.21167500 0.03656667 0.13203333 0.09546667 0.69442114 0.009703056 FALSE TRUE
ENST00000398425 ENSG00000125814 HEK293_OSMI2_2hA HEK293_TMG_2hB NAPB protein_coding protein_coding 4.161378 2.128759 5.552327 0.4410373 0.3128725 1.378914 0.88904664 0.38685851 0.9616261 0.38685851 0.1535486 1.2917765 0.20638750 0.12860000 0.17126667 0.04266667 0.47899490 0.009703056 FALSE TRUE
ENST00000432543 ENSG00000125814 HEK293_OSMI2_2hA HEK293_TMG_2hB NAPB protein_coding protein_coding 4.161378 2.128759 5.552327 0.4410373 0.3128725 1.378914 1.40451920 1.48224972 2.1101611 0.09641246 0.3285307 0.5066849 0.36439167 0.73383333 0.37593333 -0.35790000 0.02121234 0.009703056 FALSE TRUE
ENST00000617876 ENSG00000125814 HEK293_OSMI2_2hA HEK293_TMG_2hB NAPB protein_coding protein_coding 4.161378 2.128759 5.552327 0.4410373 0.3128725 1.378914 0.06596245 0.00000000 0.3775165 0.00000000 0.3775165 5.2761857 0.01602917 0.00000000 0.06883333 0.06883333 0.98684255 0.009703056 FALSE TRUE
MSTRG.20481.1 ENSG00000125814 HEK293_OSMI2_2hA HEK293_TMG_2hB NAPB protein_coding   4.161378 2.128759 5.552327 0.4410373 0.3128725 1.378914 0.34535328 0.06603371 0.7205827 0.01709507 0.1651929 3.2643365 0.07313750 0.03613333 0.13373333 0.09760000 0.11303529 0.009703056 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125814 E001 4.3059512 0.0190413754 4.251033e-03 1.384492e-02 20 23373817 23374055 239 - 0.801 0.309 -2.359
ENSG00000125814 E002 1.3513912 0.1845504045 2.853897e-01 4.257083e-01 20 23374519 23374521 3 - 0.420 0.181 -1.653
ENSG00000125814 E003 159.1876598 0.0074156566 2.903523e-08 3.277310e-07 20 23374522 23376072 1551 - 2.085 2.332 0.826
ENSG00000125814 E004 96.7796485 0.0004169382 7.059704e-01 8.042234e-01 20 23376073 23377031 959 - 1.950 2.010 0.199
ENSG00000125814 E005 53.2951305 0.0005667172 3.855441e-01 5.297373e-01 20 23377032 23377486 455 - 1.707 1.709 0.007
ENSG00000125814 E006 2.2980465 0.0066558347 3.593373e-01 5.036762e-01 20 23378799 23379444 646 - 0.539 0.407 -0.662
ENSG00000125814 E007 14.8395548 0.0035900318 2.813812e-01 4.212813e-01 20 23379445 23379471 27 - 1.191 1.128 -0.227
ENSG00000125814 E008 12.2720790 0.0296247133 2.338125e-01 3.669150e-01 20 23379472 23379495 24 - 1.130 0.995 -0.493
ENSG00000125814 E009 1.4821809 0.0092672192 1.236626e-01 2.251737e-01 20 23379496 23379866 371 - 0.454 0.181 -1.832
ENSG00000125814 E010 13.4086939 0.0094027707 8.123268e-01 8.809736e-01 20 23379867 23379935 69 - 1.130 1.143 0.047
ENSG00000125814 E011 17.7274042 0.0011147237 9.177908e-02 1.779131e-01 20 23381213 23381317 105 - 1.279 1.175 -0.366
ENSG00000125814 E012 15.6778462 0.0014119403 8.432919e-02 1.663482e-01 20 23389946 23390030 85 - 1.229 1.111 -0.423
ENSG00000125814 E013 15.3455423 0.0142282727 3.112056e-01 4.536613e-01 20 23390209 23390264 56 - 1.208 1.129 -0.282
ENSG00000125814 E014 16.9433869 0.0127480025 9.017839e-02 1.753946e-01 20 23394922 23394999 78 - 1.269 1.127 -0.502
ENSG00000125814 E015 8.8903760 0.0572315509 5.446234e-02 1.170647e-01 20 23395139 23395185 47 - 1.049 0.751 -1.139
ENSG00000125814 E016 6.2155673 0.0305238752 4.145099e-02 9.372185e-02 20 23395186 23395262 77 - 0.911 0.613 -1.204
ENSG00000125814 E017 1.4939360 0.0529498023 1.992097e-02 5.132228e-02 20 23396738 23397071 334 - 0.484 0.000 -11.094
ENSG00000125814 E018 7.6342749 0.0029100283 1.642677e-03 6.097347e-03 20 23397072 23397188 117 - 1.009 0.613 -1.569
ENSG00000125814 E019 0.2987644 0.0272531499 5.705592e-01   20 23397189 23397200 12 - 0.149 0.000 -9.150
ENSG00000125814 E020 14.1950065 0.0013117947 4.350322e-04 1.909270e-03 20 23402993 23403072 80 - 1.240 0.916 -1.177
ENSG00000125814 E021 11.0874360 0.0015406804 1.765992e-03 6.491180e-03 20 23421305 23421519 215 - 1.143 0.825 -1.180