ENSG00000125744

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000344680 ENSG00000125744 HEK293_OSMI2_2hA HEK293_TMG_2hB RTN2 protein_coding protein_coding 14.03311 23.80318 7.944847 0.8091584 0.03582068 -1.581854 1.924856 3.278688 1.741814 0.42882657 0.1101488 -0.9086623 0.1437583 0.1368667 0.2193000 0.082433333 0.055566008 0.007777668 FALSE TRUE
ENST00000430715 ENSG00000125744 HEK293_OSMI2_2hA HEK293_TMG_2hB RTN2 protein_coding protein_coding 14.03311 23.80318 7.944847 0.8091584 0.03582068 -1.581854 8.949083 15.999855 4.109816 0.02509305 0.1697109 -1.9583085 0.6264500 0.6736667 0.5174667 -0.156200000 0.007777668 0.007777668 FALSE TRUE
ENST00000588036 ENSG00000125744 HEK293_OSMI2_2hA HEK293_TMG_2hB RTN2 protein_coding processed_transcript 14.03311 23.80318 7.944847 0.8091584 0.03582068 -1.581854 1.697541 3.029615 1.014748 0.47750788 0.3421385 -1.5686186 0.1130875 0.1261667 0.1274333 0.001266667 0.969447533 0.007777668   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125744 E001 119.4945398 0.0018493660 1.770737e-02 4.650329e-02 19 45485294 45485637 344 - 1.892 1.995 0.348
ENSG00000125744 E002 37.0631040 0.0052431162 5.522857e-04 2.355445e-03 19 45485638 45485648 11 - 1.227 1.525 1.036
ENSG00000125744 E003 57.1762730 0.0026265450 2.853412e-08 3.226130e-07 19 45485649 45485681 33 - 1.312 1.715 1.380
ENSG00000125744 E004 47.2590093 0.0012222768 3.472807e-06 2.564162e-05 19 45485682 45485695 14 - 1.286 1.628 1.180
ENSG00000125744 E005 107.7129025 0.0003612866 8.644532e-04 3.488955e-03 19 45485696 45485789 94 - 1.813 1.957 0.482
ENSG00000125744 E006 1.9980464 0.3297213004 2.784127e-01 4.180990e-01 19 45485935 45486054 120 - 0.607 0.363 -1.220
ENSG00000125744 E007 122.6580555 0.0005670714 1.023737e-01 1.941380e-01 19 45486055 45486113 59 - 1.937 2.000 0.213
ENSG00000125744 E008 107.4682148 0.0002862745 2.317099e-01 3.644970e-01 19 45488471 45488517 47 - 1.969 1.920 -0.164
ENSG00000125744 E009 1.6585973 0.0083253058 2.117635e-01 3.405599e-01 19 45488518 45488636 119 - 0.537 0.310 -1.230
ENSG00000125744 E010 132.0837737 0.0016484850 2.334757e-01 3.665262e-01 19 45488637 45488706 70 - 2.054 2.007 -0.159
ENSG00000125744 E011 1.1176488 0.3090858363 7.037231e-01 8.023872e-01 19 45488707 45488759 53 - 0.347 0.265 -0.546
ENSG00000125744 E012 0.9995446 0.0125843109 7.462181e-01 8.340390e-01 19 45488760 45488847 88 - 0.207 0.271 0.506
ENSG00000125744 E013 162.0220777 0.0034111211 5.309199e-01 6.634380e-01 19 45488848 45488986 139 - 2.125 2.101 -0.081
ENSG00000125744 E014 0.0000000       19 45488987 45489103 117 -      
ENSG00000125744 E015 152.7197059 0.0004171555 5.884288e-01 7.116160e-01 19 45489346 45489553 208 - 2.094 2.075 -0.066
ENSG00000125744 E016 71.0279354 0.0004012732 9.662594e-01 9.828136e-01 19 45493160 45493378 219 - 1.752 1.748 -0.013
ENSG00000125744 E017 2.9505635 0.0054098634 1.150498e-01 2.129082e-01 19 45493886 45494117 232 - 0.722 0.464 -1.163
ENSG00000125744 E018 2.3918462 0.0472769546 1.150636e-01 2.129274e-01 19 45494118 45494165 48 - 0.669 0.380 -1.391
ENSG00000125744 E019 57.5866339 0.0005204194 1.794340e-05 1.124933e-04 19 45494166 45494420 255 - 1.829 1.608 -0.749
ENSG00000125744 E020 28.7925690 0.0008878832 1.231422e-03 4.747053e-03 19 45494526 45494554 29 - 1.546 1.314 -0.801
ENSG00000125744 E021 63.3891475 0.0037018598 2.268412e-05 1.388420e-04 19 45494555 45495005 451 - 1.878 1.643 -0.793
ENSG00000125744 E022 13.3627754 0.0056920506 7.718820e-03 2.307243e-02 19 45495095 45495139 45 - 1.257 0.980 -1.001
ENSG00000125744 E023 1.2815581 0.4229428489 4.898433e-01 6.272218e-01 19 45495140 45495214 75 - 0.450 0.241 -1.298
ENSG00000125744 E024 0.8020473 0.0965858253 4.105020e-01 5.540952e-01 19 45496591 45496791 201 - 0.346 0.182 -1.232
ENSG00000125744 E025 11.1890806 0.0015813454 4.433960e-03 1.435481e-02 19 45496792 45497055 264 - 1.211 0.909 -1.100