ENSG00000125686

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000300651 ENSG00000125686 HEK293_OSMI2_2hA HEK293_TMG_2hB MED1 protein_coding protein_coding 12.49386 6.864947 18.2589 0.9918618 0.160552 1.409969 9.9943927 4.9184425 13.66467389 0.5738004 0.31048533 1.472303 0.80567500 0.72780000 0.74880000 0.02100000 9.354646e-01 5.799034e-08 FALSE TRUE
ENST00000394287 ENSG00000125686 HEK293_OSMI2_2hA HEK293_TMG_2hB MED1 protein_coding protein_coding 12.49386 6.864947 18.2589 0.9918618 0.160552 1.409969 0.3057223 1.0209861 0.08558858 0.4219616 0.08558858 -3.431043 0.03768750 0.14203333 0.00470000 -0.13733333 4.219526e-03 5.799034e-08 FALSE TRUE
ENST00000577831 ENSG00000125686 HEK293_OSMI2_2hA HEK293_TMG_2hB MED1 protein_coding nonsense_mediated_decay 12.49386 6.864947 18.2589 0.9918618 0.160552 1.409969 0.3735005 0.0000000 1.22467362 0.0000000 0.21743879 6.947986 0.02155000 0.00000000 0.06693333 0.06693333 5.799034e-08 5.799034e-08 FALSE TRUE
MSTRG.14265.1 ENSG00000125686 HEK293_OSMI2_2hA HEK293_TMG_2hB MED1 protein_coding   12.49386 6.864947 18.2589 0.9918618 0.160552 1.409969 0.6120704 0.7313946 0.89166696 0.2232900 0.08937995 0.282353 0.05824167 0.10483333 0.04876667 -0.05606667 6.622244e-02 5.799034e-08 FALSE TRUE
MSTRG.14265.5 ENSG00000125686 HEK293_OSMI2_2hA HEK293_TMG_2hB MED1 protein_coding   12.49386 6.864947 18.2589 0.9918618 0.160552 1.409969 0.6600107 0.1624633 1.39170455 0.1015748 0.23985704 3.022821 0.04074167 0.02156667 0.07600000 0.05443333 9.857868e-02 5.799034e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125686 E001 0.2214452 0.0703056353 1.998299e-01   17 39404234 39404284 51 - 0.000 0.182 10.981
ENSG00000125686 E002 84.6390790 0.0156669329 1.341768e-15 4.993986e-14 17 39404285 39404475 191 - 1.615 2.215 2.022
ENSG00000125686 E003 136.0107862 0.0128298521 8.788951e-15 2.916008e-13 17 39404476 39404576 101 - 1.876 2.384 1.700
ENSG00000125686 E004 243.7275269 0.0076060286 3.275511e-19 1.911694e-17 17 39404577 39404997 421 - 2.166 2.610 1.481
ENSG00000125686 E005 50.1205173 0.0005162822 1.131546e-11 2.335417e-10 17 39404998 39405019 22 - 1.535 1.902 1.244
ENSG00000125686 E006 159.3028418 0.0006199267 1.894254e-32 3.813610e-30 17 39405020 39405209 190 - 2.021 2.401 1.273
ENSG00000125686 E007 110.0651230 0.0002659754 4.935236e-25 5.416950e-23 17 39405210 39405308 99 - 1.862 2.241 1.270
ENSG00000125686 E008 733.4441271 0.0011085840 8.551451e-13 2.123816e-11 17 39405309 39407713 2405 - 2.784 2.936 0.504
ENSG00000125686 E009 145.9089858 0.0019044958 4.680825e-03 1.504246e-02 17 39407714 39408058 345 - 2.163 2.082 -0.273
ENSG00000125686 E010 631.9235305 0.0072955548 1.719269e-12 4.052132e-11 17 39408059 39410386 2328 - 2.840 2.585 -0.847
ENSG00000125686 E011 69.1473091 0.0007304780 1.546825e-14 4.963436e-13 17 39410387 39410580 194 - 1.908 1.528 -1.291
ENSG00000125686 E012 83.0786805 0.0003567997 3.732453e-07 3.386018e-06 17 39410581 39410721 141 - 1.952 1.756 -0.660
ENSG00000125686 E013 79.9427916 0.0028587342 9.122665e-05 4.807939e-04 17 39415026 39415131 106 - 1.931 1.759 -0.579
ENSG00000125686 E014 67.7156702 0.0003556865 4.088035e-08 4.488357e-07 17 39415244 39415339 96 - 1.877 1.634 -0.822
ENSG00000125686 E015 97.9241432 0.0002663066 2.306420e-09 3.197778e-08 17 39419717 39419918 202 - 2.027 1.812 -0.725
ENSG00000125686 E016 72.8186089 0.0029799952 5.325753e-06 3.766137e-05 17 39423327 39423445 119 - 1.900 1.678 -0.748
ENSG00000125686 E017 78.6231241 0.0003318697 8.179649e-09 1.024986e-07 17 39423697 39423821 125 - 1.938 1.701 -0.798
ENSG00000125686 E018 72.9927668 0.0003728222 9.845737e-10 1.462020e-08 17 39424627 39424738 112 - 1.912 1.644 -0.904
ENSG00000125686 E019 0.0000000       17 39427446 39427700 255 -      
ENSG00000125686 E020 58.3160355 0.0004096415 8.535221e-09 1.065690e-07 17 39427701 39427790 90 - 1.819 1.534 -0.966
ENSG00000125686 E021 55.1966173 0.0004004328 3.943071e-06 2.872205e-05 17 39431115 39431188 74 - 1.782 1.560 -0.756
ENSG00000125686 E022 0.1472490 0.0444882009 1.000000e+00   17 39431189 39431236 48 - 0.081 0.000 -12.767
ENSG00000125686 E023 52.7043993 0.0004566824 1.881193e-07 1.811919e-06 17 39431942 39432016 75 - 1.773 1.507 -0.907
ENSG00000125686 E024 46.3289782 0.0005986493 4.136919e-09 5.469516e-08 17 39434249 39434320 72 - 1.733 1.396 -1.153
ENSG00000125686 E025 38.5775113 0.0005924274 8.144561e-05 4.348225e-04 17 39439165 39439193 29 - 1.632 1.405 -0.778
ENSG00000125686 E026 65.8227342 0.0010424080 6.891235e-07 5.925580e-06 17 39440386 39440518 133 - 1.859 1.628 -0.779
ENSG00000125686 E027 49.3162894 0.0044823571 8.484109e-06 5.729677e-05 17 39440623 39440677 55 - 1.744 1.470 -0.934
ENSG00000125686 E028 57.9105894 0.0013402596 2.426350e-07 2.288711e-06 17 39443550 39443628 79 - 1.811 1.547 -0.895
ENSG00000125686 E029 2.2195720 0.0289984075 3.290044e-03 1.112061e-02 17 39445411 39445569 159 - 0.609 0.000 -16.786
ENSG00000125686 E030 68.0689704 0.0080748908 1.825231e-04 8.887463e-04 17 39447798 39447904 107 - 1.872 1.648 -0.759
ENSG00000125686 E031 71.6074523 0.0032117287 2.744173e-07 2.559198e-06 17 39451038 39451301 264 - 1.903 1.643 -0.877