ENSG00000125651

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394456 ENSG00000125651 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2F1 protein_coding protein_coding 86.51227 133.1037 71.22119 3.384904 0.7152874 -0.902078 79.076410 125.6758 59.317898 4.047723 1.174117 -1.083039 0.90508750 0.9439 0.8329667 -0.1109333 9.060069e-07 2.01966e-26 FALSE TRUE
ENST00000598607 ENSG00000125651 HEK293_OSMI2_2hA HEK293_TMG_2hB GTF2F1 protein_coding protein_coding 86.51227 133.1037 71.22119 3.384904 0.7152874 -0.902078 2.564027 0.0000 7.748733 0.000000 1.525128 9.599677 0.03707917 0.0000 0.1088667 0.1088667 2.019660e-26 2.01966e-26 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125651 E001 1041.411786 1.333666e-04 2.435540e-45 1.156406e-42 19 6379572 6379877 306 - 2.815 2.999 0.611
ENSG00000125651 E002 1682.873159 3.434587e-04 2.334842e-09 3.233635e-08 19 6379878 6380189 312 - 3.119 3.182 0.208
ENSG00000125651 E003 1245.846956 1.000244e-03 7.210594e-02 1.468426e-01 19 6380190 6380390 201 - 3.021 3.041 0.067
ENSG00000125651 E004 777.739604 4.054886e-04 5.935329e-02 1.255309e-01 19 6380391 6380475 85 - 2.818 2.837 0.063
ENSG00000125651 E005 472.299801 1.695182e-03 3.737284e-01 5.181360e-01 19 6380476 6380485 10 - 2.608 2.620 0.041
ENSG00000125651 E006 851.367816 2.106224e-04 2.142179e-02 5.447813e-02 19 6380573 6380690 118 - 2.856 2.877 0.071
ENSG00000125651 E007 868.703045 9.733813e-05 5.430877e-08 5.808521e-07 19 6380904 6381039 136 - 2.831 2.895 0.212
ENSG00000125651 E008 376.220605 2.120793e-04 9.533406e-04 3.799464e-03 19 6381040 6381042 3 - 2.471 2.530 0.194
ENSG00000125651 E009 10.334582 3.740321e-03 7.533504e-02 1.520562e-01 19 6381043 6381121 79 - 1.136 0.931 -0.751
ENSG00000125651 E010 721.778423 1.022685e-04 1.576377e-03 5.882329e-03 19 6381122 6381195 74 - 2.772 2.807 0.117
ENSG00000125651 E011 626.809545 9.995961e-04 9.399491e-03 2.728005e-02 19 6381359 6381392 34 - 2.704 2.748 0.145
ENSG00000125651 E012 521.269586 4.045754e-03 2.891096e-01 4.297918e-01 19 6381393 6381412 20 - 2.639 2.664 0.085
ENSG00000125651 E013 758.909658 9.670240e-04 7.515754e-01 8.379751e-01 19 6381413 6381478 66 - 2.836 2.816 -0.066
ENSG00000125651 E014 10.735969 1.611340e-03 9.529787e-01 9.745640e-01 19 6381479 6381553 75 - 1.027 1.008 -0.068
ENSG00000125651 E015 682.004376 6.658470e-04 1.308429e-01 2.353257e-01 19 6381554 6381612 59 - 2.806 2.763 -0.142
ENSG00000125651 E016 414.240888 1.610580e-04 1.696235e-01 2.877105e-01 19 6381613 6381615 3 - 2.587 2.547 -0.135
ENSG00000125651 E017 954.800567 1.183286e-04 1.420276e-01 2.508129e-01 19 6381697 6381821 125 - 2.945 2.912 -0.109
ENSG00000125651 E018 524.456026 1.753608e-04 5.256349e-01 6.589855e-01 19 6381822 6381850 29 - 2.662 2.660 -0.006
ENSG00000125651 E019 1061.952774 1.916924e-04 1.016874e-01 1.930645e-01 19 6383311 6383495 185 - 2.992 2.957 -0.117
ENSG00000125651 E020 821.916869 1.115650e-04 5.836561e-05 3.233583e-04 19 6387389 6387504 116 - 2.906 2.837 -0.231
ENSG00000125651 E021 665.220072 9.290596e-04 9.314823e-04 3.724730e-03 19 6387505 6387559 55 - 2.824 2.742 -0.272
ENSG00000125651 E022 1102.517888 1.571994e-03 7.988536e-07 6.773505e-06 19 6389444 6389637 194 - 3.069 2.949 -0.399
ENSG00000125651 E023 614.122949 2.024357e-03 4.137393e-08 4.539128e-07 19 6391902 6391974 73 - 2.840 2.683 -0.521
ENSG00000125651 E024 1.839157 5.516576e-02 4.027646e-01 5.465525e-01 19 6392203 6392410 208 - 0.548 0.388 -0.812
ENSG00000125651 E025 510.245384 2.991392e-03 7.235494e-05 3.915538e-04 19 6392857 6392903 47 - 2.746 2.610 -0.453
ENSG00000125651 E026 644.495487 5.264369e-03 3.253850e-04 1.482450e-03 19 6392984 6393141 158 - 2.854 2.707 -0.490
ENSG00000125651 E027 282.527945 4.664173e-03 1.164229e-03 4.519384e-03 19 6393142 6393431 290 - 2.493 2.350 -0.478
ENSG00000125651 E028 2.290668 3.383154e-02 5.401388e-01 6.713225e-01 19 6393941 6393981 41 - 0.548 0.428 -0.593