ENSG00000125485

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372159 ENSG00000125485 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX31 protein_coding protein_coding 12.4831 10.86626 14.62063 0.9950731 0.4057161 0.4278097 5.897334 6.8159418 5.558206 0.70784004 0.3329411 -0.2938155 0.4995500 0.62873333 0.3795000 -0.2492333 1.246973e-04 1.332074e-26 FALSE TRUE
MSTRG.33557.3 ENSG00000125485 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX31 protein_coding   12.4831 10.86626 14.62063 0.9950731 0.4057161 0.4278097 1.611974 0.0000000 2.586871 0.00000000 0.1913916 8.0206305 0.1136167 0.00000000 0.1769667 0.1769667 1.332074e-26 1.332074e-26 FALSE TRUE
MSTRG.33557.8 ENSG00000125485 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX31 protein_coding   12.4831 10.86626 14.62063 0.9950731 0.4057161 0.4278097 2.468279 2.4396455 2.986087 0.05107788 0.5246942 0.2905066 0.1986000 0.22793333 0.2053333 -0.0226000 8.490791e-01 1.332074e-26 FALSE TRUE
MSTRG.33557.9 ENSG00000125485 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX31 protein_coding   12.4831 10.86626 14.62063 0.9950731 0.4057161 0.4278097 1.410387 0.2797619 2.123395 0.27976192 0.3411779 2.8802111 0.0991750 0.02323333 0.1455333 0.1223000 8.718014e-02 1.332074e-26 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125485 E001 4.6214384 0.0937590900 5.436311e-02 1.168845e-01 9 132537460 132537468 9 - 0.513 0.867 1.496
ENSG00000125485 E002 6.2287422 0.1391233124 1.375852e-01 2.447198e-01 9 132537469 132537573 105 - 0.686 0.957 1.062
ENSG00000125485 E003 1.9404099 0.0116619912 9.884796e-01 9.968460e-01 9 132537574 132537579 6 - 0.468 0.472 0.022
ENSG00000125485 E004 3.5487564 0.0906933457 3.449486e-01 4.890367e-01 9 132537580 132537646 67 - 0.554 0.724 0.737
ENSG00000125485 E005 168.3540914 0.0014959194 1.720790e-08 2.031004e-07 9 132592997 132594410 1414 - 2.122 2.305 0.613
ENSG00000125485 E006 174.1334530 0.0029881192 3.505626e-06 2.585473e-05 9 132594411 132594673 263 - 2.144 2.317 0.580
ENSG00000125485 E007 118.6162428 0.0002796922 8.777982e-09 1.093608e-07 9 132594674 132594786 113 - 1.966 2.158 0.644
ENSG00000125485 E008 210.4925723 0.0002612998 6.512103e-04 2.720033e-03 9 132594787 132595112 326 - 2.278 2.364 0.288
ENSG00000125485 E009 156.2068854 0.0016306769 3.436611e-01 4.877215e-01 9 132612087 132612255 169 - 2.181 2.217 0.118
ENSG00000125485 E010 0.6727692 0.0968463756 9.849766e-01 9.946370e-01 9 132612256 132612313 58 - 0.217 0.219 0.021
ENSG00000125485 E011 99.4211517 0.0026930872 7.237714e-01 8.175116e-01 9 132618330 132618417 88 - 1.996 2.017 0.069
ENSG00000125485 E012 58.0426712 0.0025499736 3.576718e-01 5.020885e-01 9 132618418 132618441 24 - 1.799 1.757 -0.144
ENSG00000125485 E013 77.5119795 0.0076632573 7.697281e-01 8.512956e-01 9 132625664 132625745 82 - 1.886 1.911 0.085
ENSG00000125485 E014 91.8528635 0.0003042106 8.076359e-01 8.777857e-01 9 132630264 132630378 115 - 1.963 1.975 0.041
ENSG00000125485 E015 52.6225464 0.0004845263 3.030539e-01 4.449696e-01 9 132630379 132630403 25 - 1.755 1.709 -0.156
ENSG00000125485 E016 71.5664754 0.0004488371 3.441958e-02 8.056296e-02 9 132632041 132632091 51 - 1.907 1.823 -0.284
ENSG00000125485 E017 0.1482932 0.0409257569 4.801472e-01   9 132637863 132638363 501 - 0.000 0.123 9.327
ENSG00000125485 E018 74.4735888 0.0053674795 6.772974e-02 1.395860e-01 9 132642004 132642063 60 - 1.931 1.835 -0.322
ENSG00000125485 E019 114.6260338 0.0038761641 1.856591e-01 3.083180e-01 9 132645895 132646071 177 - 2.096 2.038 -0.193
ENSG00000125485 E020 175.9959253 0.0002064919 3.600167e-03 1.201157e-02 9 132646823 132647058 236 - 2.288 2.215 -0.244
ENSG00000125485 E021 119.7073850 0.0002894191 6.357715e-01 7.499688e-01 9 132648189 132648295 107 - 2.093 2.081 -0.038
ENSG00000125485 E022 120.0612886 0.0003085985 8.917530e-01 9.348832e-01 9 132648432 132648551 120 - 2.087 2.086 -0.003
ENSG00000125485 E023 99.0004512 0.0022514005 9.450416e-02 1.821352e-01 9 132650234 132650298 65 - 2.038 1.973 -0.219
ENSG00000125485 E024 75.3306353 0.0036622245 2.430233e-02 6.043823e-02 9 132651075 132651112 38 - 1.939 1.831 -0.365
ENSG00000125485 E025 48.4974956 0.0005100266 2.355501e-02 5.889437e-02 9 132651113 132651116 4 - 1.750 1.640 -0.374
ENSG00000125485 E026 78.2668387 0.0003706096 4.614319e-03 1.485924e-02 9 132652448 132652492 45 - 1.957 1.848 -0.365
ENSG00000125485 E027 7.6189564 0.0022790816 1.806909e-02 4.727891e-02 9 132658081 132658358 278 - 0.767 1.048 1.068
ENSG00000125485 E028 83.2026964 0.0004249741 2.521891e-01 3.885119e-01 9 132658671 132658735 65 - 1.950 1.910 -0.136
ENSG00000125485 E029 80.9713476 0.0043195297 5.516635e-01 6.810752e-01 9 132659710 132659780 71 - 1.930 1.902 -0.094
ENSG00000125485 E030 0.1515154 0.0438544035 5.014074e-01   9 132660756 132660789 34 - 0.122 0.000 -9.563
ENSG00000125485 E031 1.1060972 0.0116324916 2.594441e-01 3.968328e-01 9 132661076 132661207 132 - 0.417 0.219 -1.300
ENSG00000125485 E032 68.7794974 0.0004795656 6.179696e-01 7.357081e-01 9 132661208 132661251 44 - 1.854 1.836 -0.061
ENSG00000125485 E033 70.7088030 0.0016065728 9.063372e-01 9.444890e-01 9 132662261 132662336 76 - 1.858 1.854 -0.012
ENSG00000125485 E034 140.4866871 0.0014076798 1.738343e-04 8.520006e-04 9 132662439 132662695 257 - 2.214 2.088 -0.421
ENSG00000125485 E035 1.7348593 0.0080476388 3.746527e-01 5.190680e-01 9 132669570 132669673 104 - 0.513 0.364 -0.786
ENSG00000125485 E036 1.3243464 0.4647440648 8.226917e-01 8.882196e-01 9 132669674 132669859 186 - 0.361 0.367 0.033
ENSG00000125485 E037 82.9090896 0.0027084255 1.231001e-05 8.009664e-05 9 132669860 132670401 542 - 2.019 1.819 -0.670