ENSG00000125458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000245552 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding protein_coding 94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 54.075265 114.061436 31.212012 7.6666613 0.9134530 -1.8693022 0.52841250 0.61990000 0.60663333 -0.01326667 9.082563e-01 2.936436e-06 FALSE  
ENST00000578337 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding protein_coding 94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 4.496820 6.014885 2.986613 0.9964705 0.5764284 -1.0076009 0.05416667 0.03230000 0.05810000 0.02580000 1.543362e-01 2.936436e-06    
ENST00000580423 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding protein_coding 94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 5.132935 12.918125 2.005148 1.6653674 0.5542568 -2.6815557 0.05047917 0.07033333 0.03916667 -0.03116667 3.053053e-01 2.936436e-06    
ENST00000582160 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding protein_coding 94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 6.712488 12.522193 1.322611 0.8557534 0.3224426 -3.2333108 0.07693750 0.06806667 0.02586667 -0.04220000 3.533631e-03 2.936436e-06 FALSE  
ENST00000583655 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding retained_intron 94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 4.192184 4.561573 2.379284 1.1434646 0.6813897 -0.9361125 0.05380833 0.02426667 0.04613333 0.02186667 3.511016e-01 2.936436e-06    
ENST00000584352 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding retained_intron 94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 10.026206 11.274079 7.739034 2.0125474 0.5152389 -0.5422004 0.13764583 0.06070000 0.15050000 0.08980000 4.355021e-05 2.936436e-06    
MSTRG.15053.9 ENSG00000125458 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C protein_coding   94.27696 183.9903 51.44329 10.05845 0.5895215 -1.838373 5.482637 14.156721 2.147923 1.8203930 1.4463174 -2.7147904 0.05574167 0.07830000 0.04160000 -0.03670000 3.451934e-01 2.936436e-06    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125458 E001 1.652153 8.733897e-03 9.155125e-01 9.505288e-01 17 75130225 75130227 3 - 0.375 0.349 -0.154
ENSG00000125458 E002 6.815672 3.622047e-02 5.989404e-01 7.204375e-01 17 75130228 75130229 2 - 0.709 0.801 0.370
ENSG00000125458 E003 156.650419 2.175963e-04 2.497671e-02 6.182643e-02 17 75130230 75130270 41 - 2.132 2.043 -0.298
ENSG00000125458 E004 295.589049 1.639931e-04 2.818990e-01 4.218531e-01 17 75130271 75130331 61 - 2.314 2.335 0.071
ENSG00000125458 E005 369.917500 1.806944e-04 7.591633e-04 3.112028e-03 17 75130332 75130362 31 - 2.363 2.441 0.259
ENSG00000125458 E006 608.865792 1.605715e-04 7.466688e-05 4.026248e-04 17 75130363 75130435 73 - 2.585 2.656 0.236
ENSG00000125458 E007 617.134433 1.364879e-04 3.699468e-04 1.657969e-03 17 75130436 75130472 37 - 2.598 2.660 0.204
ENSG00000125458 E008 950.075866 9.183483e-05 7.568757e-02 1.526052e-01 17 75130473 75130545 73 - 2.821 2.840 0.062
ENSG00000125458 E009 718.113423 1.811234e-04 8.027441e-01 8.743702e-01 17 75130546 75130567 22 - 2.727 2.715 -0.041
ENSG00000125458 E010 564.760627 3.683250e-04 3.396274e-01 4.835517e-01 17 75130568 75130570 3 - 2.637 2.609 -0.091
ENSG00000125458 E011 911.802928 2.020951e-04 7.734910e-01 8.538553e-01 17 75130571 75130637 67 - 2.823 2.820 -0.010
ENSG00000125458 E012 549.453322 1.283937e-04 5.621166e-01 6.897816e-01 17 75130638 75130642 5 - 2.598 2.601 0.011
ENSG00000125458 E013 71.627132 1.593055e-03 5.317660e-06 3.761115e-05 17 75130643 75130752 110 - 1.917 1.674 -0.820
ENSG00000125458 E014 694.315715 3.029743e-04 2.448172e-01 3.798174e-01 17 75130753 75130787 35 - 2.726 2.697 -0.097
ENSG00000125458 E015 817.216104 1.100917e-04 7.192319e-01 8.141268e-01 17 75130788 75130867 80 - 2.784 2.770 -0.047
ENSG00000125458 E016 63.821410 6.583098e-03 1.145007e-09 1.678803e-08 17 75130868 75130964 97 - 1.965 1.585 -1.283
ENSG00000125458 E017 94.125490 2.088669e-02 1.310758e-02 3.615912e-02 17 75130965 75131044 80 - 1.999 1.800 -0.668
ENSG00000125458 E018 533.630320 1.227210e-04 6.150360e-02 1.291674e-01 17 75131045 75131081 37 - 2.561 2.591 0.100
ENSG00000125458 E019 428.516373 1.403130e-04 2.413158e-01 3.757330e-01 17 75131082 75131105 24 - 2.476 2.495 0.061
ENSG00000125458 E020 37.350051 2.193123e-02 3.604762e-03 1.202372e-02 17 75131106 75131180 75 - 1.671 1.385 -0.980
ENSG00000125458 E021 640.504282 1.258276e-03 5.793010e-01 7.042276e-01 17 75131181 75131281 101 - 2.661 2.667 0.023
ENSG00000125458 E022 103.996099 7.898318e-03 8.862671e-03 2.593941e-02 17 75131282 75131533 252 - 2.011 1.850 -0.541
ENSG00000125458 E023 353.130663 1.129120e-02 3.092920e-02 7.377795e-02 17 75131534 75131757 224 - 2.508 2.389 -0.400