ENSG00000125447

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000537686 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding protein_coding 34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 11.748284 14.8636267 6.338814 0.53359141 0.4690414 -1.2281975 0.33357083 0.42846667 0.25456667 -0.173900000 2.434779e-05 1.538093e-08 FALSE TRUE
ENST00000538886 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding protein_coding 34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 4.213741 8.1252565 1.134824 0.71797817 1.1348236 -2.8290625 0.11916250 0.23433333 0.04660000 -0.187733333 8.105852e-02 1.538093e-08 FALSE TRUE
ENST00000578208 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding retained_intron 34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 4.799031 3.8913145 3.465499 0.68112217 0.0195406 -0.1667402 0.13816667 0.11043333 0.13930000 0.028866667 3.765160e-01 1.538093e-08 FALSE TRUE
ENST00000582821 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding retained_intron 34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 1.316174 0.4384557 1.597628 0.43845572 0.1879701 1.8418962 0.04202083 0.01123333 0.06406667 0.052833333 9.190543e-02 1.538093e-08 FALSE TRUE
ENST00000613421 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding retained_intron 34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 2.042545 0.6920257 1.935860 0.06279722 0.2439229 1.4708122 0.06085417 0.02006667 0.07803333 0.057966667 2.034439e-06 1.538093e-08 TRUE TRUE
ENST00000621870 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding nonsense_mediated_decay 34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 1.875394 1.6324218 1.368530 0.17431107 0.1861497 -0.2526945 0.05453750 0.04713333 0.05496667 0.007833333 7.853222e-01 1.538093e-08 FALSE TRUE
MSTRG.15067.9 ENSG00000125447 HEK293_OSMI2_2hA HEK293_TMG_2hB GGA3 protein_coding   34.03384 34.85757 24.8845 2.323725 0.3082394 -0.4860585 1.297987 0.0000000 2.447165 0.00000000 1.2514713 7.9408511 0.04508333 0.00000000 0.09720000 0.097200000 1.524567e-01 1.538093e-08 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125447 E001 1.3974828 0.0545882124 3.429902e-02 8.032841e-02 17 75236599 75236599 1 - 0.000 0.429 12.105
ENSG00000125447 E002 1.3974828 0.0545882124 3.429902e-02 8.032841e-02 17 75236600 75236601 2 - 0.000 0.429 12.107
ENSG00000125447 E003 10.4338375 0.0252536759 1.960588e-03 7.102481e-03 17 75236602 75236620 19 - 0.660 1.119 1.766
ENSG00000125447 E004 128.9983595 0.0041687269 6.174113e-16 2.396859e-14 17 75236621 75236790 170 - 1.757 2.171 1.388
ENSG00000125447 E005 460.0992301 0.0036999047 1.873060e-06 1.466426e-05 17 75236791 75237185 395 - 2.506 2.673 0.556
ENSG00000125447 E006 264.0160796 0.0010432725 1.436278e-02 3.905198e-02 17 75237186 75237283 98 - 2.336 2.409 0.243
ENSG00000125447 E007 290.4276336 0.0033626687 3.504331e-02 8.173206e-02 17 75237284 75237426 143 - 2.371 2.452 0.270
ENSG00000125447 E008 218.3224515 0.0019943454 4.669560e-03 1.501008e-02 17 75237427 75237521 95 - 2.234 2.334 0.335
ENSG00000125447 E009 107.0735304 0.0002770661 1.104087e-02 3.125844e-02 17 75237522 75237525 4 - 1.927 2.026 0.334
ENSG00000125447 E010 109.3125728 0.0002790531 4.183248e-03 1.365891e-02 17 75237526 75237532 7 - 1.927 2.037 0.372
ENSG00000125447 E011 219.2169226 0.0025146674 2.132200e-03 7.634310e-03 17 75237533 75237644 112 - 2.225 2.339 0.382
ENSG00000125447 E012 188.4228778 0.0030271509 7.134188e-05 3.866987e-04 17 75237645 75237777 133 - 2.126 2.286 0.536
ENSG00000125447 E013 80.1606234 0.0004732910 7.521358e-03 2.256836e-02 17 75237778 75237796 19 - 1.787 1.908 0.409
ENSG00000125447 E014 110.6121233 0.0003549811 2.869252e-03 9.880775e-03 17 75237797 75237981 185 - 1.927 2.042 0.388
ENSG00000125447 E015 166.3987280 0.0001974137 2.319508e-02 5.815561e-02 17 75237982 75238090 109 - 2.136 2.207 0.240
ENSG00000125447 E016 200.7677295 0.0001825347 8.874294e-02 1.731955e-01 17 75238091 75238178 88 - 2.232 2.282 0.167
ENSG00000125447 E017 177.2576779 0.0002420197 2.762568e-02 6.726476e-02 17 75238179 75238237 59 - 2.167 2.235 0.228
ENSG00000125447 E018 111.3581606 0.0002727106 1.404063e-01 2.485892e-01 17 75238238 75238245 8 - 1.972 2.030 0.193
ENSG00000125447 E019 194.1441368 0.0003305552 1.025149e-02 2.938457e-02 17 75238246 75238356 111 - 2.196 2.273 0.258
ENSG00000125447 E020 98.0302536 0.0002948623 2.636325e-01 4.015468e-01 17 75238357 75238389 33 - 1.924 1.971 0.158
ENSG00000125447 E021 42.2191366 0.0038718507 8.786985e-03 2.575052e-02 17 75238390 75238651 262 - 1.700 1.542 -0.538
ENSG00000125447 E022 201.0254762 0.0013775949 5.358745e-02 1.155262e-01 17 75238652 75238762 111 - 2.218 2.285 0.222
ENSG00000125447 E023 139.9339961 0.0019749561 3.079124e-01 4.501481e-01 17 75238914 75238964 51 - 2.078 2.121 0.145
ENSG00000125447 E024 129.1805122 0.0025252791 3.369774e-01 4.808379e-01 17 75238965 75239018 54 - 2.044 2.088 0.146
ENSG00000125447 E025 141.2560717 0.0002632773 1.949422e-02 5.040009e-02 17 75239019 75239079 61 - 2.056 2.137 0.270
ENSG00000125447 E026 82.3118372 0.0010492404 2.597480e-01 3.972014e-01 17 75239080 75239083 4 - 1.846 1.900 0.181
ENSG00000125447 E027 5.4414501 0.0039362991 4.721317e-03 1.515408e-02 17 75239084 75239374 291 - 0.997 0.636 -1.425
ENSG00000125447 E028 188.8351434 0.0002247791 6.344216e-03 1.951902e-02 17 75239375 75239571 197 - 2.181 2.263 0.272
ENSG00000125447 E029 5.3329109 0.0030738754 2.703924e-03 9.385826e-03 17 75239572 75239788 217 - 0.997 0.611 -1.531
ENSG00000125447 E030 230.0355568 0.0003440635 1.934980e-02 5.009103e-02 17 75239789 75240108 320 - 2.277 2.343 0.219
ENSG00000125447 E031 7.1044937 0.0066594013 1.859789e-06 1.457300e-05 17 75240109 75240341 233 - 1.184 0.611 -2.209
ENSG00000125447 E032 135.0495699 0.0003307680 9.280868e-01 9.587292e-01 17 75240342 75240412 71 - 2.089 2.096 0.023
ENSG00000125447 E033 30.1193106 0.0008190718 1.088586e-12 2.653135e-11 17 75240413 75240793 381 - 1.711 1.263 -1.540
ENSG00000125447 E034 8.6686280 0.0107009564 1.277535e-02 3.538638e-02 17 75240794 75240811 18 - 1.130 0.842 -1.071
ENSG00000125447 E035 168.6594011 0.0004037481 2.458444e-01 3.810647e-01 17 75240812 75240948 137 - 2.212 2.181 -0.103
ENSG00000125447 E036 143.2757077 0.0018165216 6.104398e-01 7.297613e-01 17 75240949 75241057 109 - 2.131 2.117 -0.048
ENSG00000125447 E037 158.6263371 0.0019137722 1.348697e-02 3.705013e-02 17 75241400 75241516 117 - 2.223 2.140 -0.278
ENSG00000125447 E038 10.4306634 0.0018751958 9.404126e-08 9.597499e-07 17 75241517 75241614 98 - 1.315 0.777 -1.977
ENSG00000125447 E039 134.0858347 0.0055207589 5.649518e-05 3.140782e-04 17 75241615 75241668 54 - 2.216 2.032 -0.615
ENSG00000125447 E040 112.9029843 0.0041098626 2.160213e-05 1.328677e-04 17 75241669 75241696 28 - 2.144 1.956 -0.629
ENSG00000125447 E041 25.3458140 0.0049285154 5.508456e-20 3.490169e-18 17 75241697 75241864 168 - 1.737 1.040 -2.425
ENSG00000125447 E042 43.0838395 0.0075898594 8.054639e-22 6.276953e-20 17 75241865 75242335 471 - 1.942 1.288 -2.235
ENSG00000125447 E043 216.2669263 0.0011622032 8.010300e-09 1.005384e-07 17 75242336 75242473 138 - 2.407 2.244 -0.543
ENSG00000125447 E044 148.3338991 0.0002579822 6.062012e-10 9.365763e-09 17 75242831 75242883 53 - 2.254 2.072 -0.609
ENSG00000125447 E045 102.0385080 0.0003073964 4.158922e-09 5.496667e-08 17 75242884 75242888 5 - 2.108 1.901 -0.694
ENSG00000125447 E046 104.2524772 0.0004048483 1.060370e-10 1.863488e-09 17 75242889 75242911 23 - 2.127 1.900 -0.763
ENSG00000125447 E047 120.9868247 0.0002957920 2.212387e-06 1.704335e-05 17 75243063 75243134 72 - 2.148 1.994 -0.517
ENSG00000125447 E048 93.3326840 0.0005474131 1.742408e-04 8.536626e-04 17 75243135 75243166 32 - 2.032 1.892 -0.472
ENSG00000125447 E049 3.2829322 0.0048531511 1.704631e-05 1.073987e-04 17 75243167 75243258 92 - 0.934 0.290 -2.997
ENSG00000125447 E050 140.5882234 0.0039191437 2.160555e-04 1.032830e-03 17 75243447 75243516 70 - 2.216 2.063 -0.512
ENSG00000125447 E051 119.1563526 0.0065855996 6.202569e-02 1.300330e-01 17 75243517 75243570 54 - 2.105 2.014 -0.304
ENSG00000125447 E052 0.8899626 0.0796098461 1.316926e-01 2.364947e-01 17 75244402 75244490 89 - 0.445 0.167 -1.924
ENSG00000125447 E053 103.0252391 0.0066244214 1.679427e-01 2.855242e-01 17 75244619 75244717 99 - 2.027 1.959 -0.226
ENSG00000125447 E054 98.9021706 0.0078781591 3.960339e-01 5.398084e-01 17 75246509 75246584 76 - 1.988 1.947 -0.137
ENSG00000125447 E055 91.1935567 0.0170755104 2.537517e-01 3.903414e-01 17 75246712 75246796 85 - 1.974 1.903 -0.239
ENSG00000125447 E056 12.5607452 0.0016260381 1.284714e-01 2.320296e-01 17 75248840 75249008 169 - 1.184 1.041 -0.517
ENSG00000125447 E057 52.7229056 0.0255628852 4.663509e-01 6.059089e-01 17 75261548 75261631 84 - 1.726 1.674 -0.178
ENSG00000125447 E058 0.9806312 0.8588654011 6.970591e-01 7.972937e-01 17 75262282 75262363 82 - 0.367 0.236 -0.879