ENSG00000125355

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371369 ENSG00000125355 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM255A protein_coding protein_coding 2.358747 3.551554 2.412652 0.3290785 0.2711779 -0.5559199 1.1157122 1.232395 1.76337992 0.2032781 0.04293389 0.5133782 0.52530000 0.3489333 0.75320000 0.40426667 3.622413e-02 4.840329e-08 FALSE TRUE
ENST00000440464 ENSG00000125355 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM255A protein_coding protein_coding 2.358747 3.551554 2.412652 0.3290785 0.2711779 -0.5559199 0.1178438 0.000000 0.09001166 0.0000000 0.06297902 3.3220963 0.05462917 0.0000000 0.03526667 0.03526667 1.971971e-01 4.840329e-08 FALSE TRUE
ENST00000480821 ENSG00000125355 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM255A protein_coding processed_transcript 2.358747 3.551554 2.412652 0.3290785 0.2711779 -0.5559199 0.9328893 2.319159 0.28220162 0.2994936 0.09290440 -2.9947733 0.34379167 0.6510667 0.11410000 -0.53696667 4.840329e-08 4.840329e-08   FALSE
ENST00000519908 ENSG00000125355 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM255A protein_coding protein_coding 2.358747 3.551554 2.412652 0.3290785 0.2711779 -0.5559199 0.1455794 0.000000 0.27705850 0.0000000 0.27705850 4.8432729 0.06179167 0.0000000 0.09746667 0.09746667 7.589100e-01 4.840329e-08 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125355 E001 0.1482932 0.041159753 3.860644e-01   X 120258650 120258650 1 - 0.000 0.142 9.717
ENSG00000125355 E002 100.8276191 0.008506620 2.716143e-02 6.632533e-02 X 120258651 120261028 2378 - 1.951 2.074 0.415
ENSG00000125355 E003 13.0166894 0.001503673 2.971392e-02 7.139504e-02 X 120268244 120268362 119 - 1.214 1.058 -0.558
ENSG00000125355 E004 8.1606329 0.002182531 2.410433e-02 6.002777e-02 X 120268363 120268387 25 - 1.047 0.832 -0.808
ENSG00000125355 E005 13.0892482 0.001541627 3.060925e-02 7.316465e-02 X 120276885 120277047 163 - 1.214 1.058 -0.558
ENSG00000125355 E006 10.8693202 0.001912133 2.046899e-04 9.840853e-04 X 120285127 120285215 89 - 1.199 0.856 -1.260
ENSG00000125355 E007 0.0000000       X 120285625 120285696 72 -      
ENSG00000125355 E008 0.0000000       X 120285887 120285991 105 -      
ENSG00000125355 E009 11.6876233 0.001616839 1.661776e-02 4.409534e-02 X 120287154 120287222 69 - 1.183 0.995 -0.683
ENSG00000125355 E010 12.2569222 0.001513937 8.562815e-02 1.684003e-01 X 120291251 120291340 90 - 1.176 1.058 -0.423
ENSG00000125355 E011 9.1976410 0.001983977 1.821433e-01 3.038923e-01 X 120293989 120294051 63 - 1.058 0.959 -0.362
ENSG00000125355 E012 13.0321086 0.001372059 4.311403e-04 1.894120e-03 X 120304349 120304491 143 - 1.262 0.977 -1.024
ENSG00000125355 E013 11.2252994 0.011799089 3.076380e-13 8.153483e-12 X 120309893 120310128 236 - 0.422 1.363 3.746
ENSG00000125355 E014 22.9473268 0.012465114 5.899460e-03 1.834504e-02 X 120311252 120311556 305 - 1.235 1.499 0.919