ENSG00000125319

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000245382 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding protein_coding 12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 1.7675438 2.7590867 1.0403027 0.3067777 0.09113194 -1.39860506 0.13910000 0.148633333 0.1317333 -0.01690000 0.825061195 0.009134908 FALSE TRUE
ENST00000319977 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding protein_coding 12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 0.6389257 0.9583346 0.3510006 0.1680947 0.23418810 -1.42350421 0.05742083 0.051300000 0.0413000 -0.01000000 0.769953752 0.009134908 FALSE TRUE
ENST00000585683 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding protein_coding 12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 4.6450657 7.2979709 2.5293363 0.9519123 0.15343194 -1.52501941 0.37509583 0.389833333 0.3210333 -0.06880000 0.593971265 0.009134908 FALSE TRUE
ENST00000588073 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding retained_intron 12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 1.9138968 3.1613333 0.7182621 0.3034938 0.24272329 -2.12255976 0.13528750 0.170433333 0.0873000 -0.08313333 0.190159683 0.009134908 FALSE TRUE
MSTRG.14430.1 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding   12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 0.5010015 0.1328470 1.0598473 0.1328470 0.24657044 2.90486255 0.04438333 0.006933333 0.1306333 0.12370000 0.009134908 0.009134908 FALSE TRUE
MSTRG.14430.4 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding   12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 1.1111703 1.1490700 1.1122497 0.5535530 0.55909076 -0.04657402 0.09804583 0.062033333 0.1343333 0.07230000 0.893992478 0.009134908 FALSE TRUE
MSTRG.14430.5 ENSG00000125319 HEK293_OSMI2_2hA HEK293_TMG_2hB HROB protein_coding   12.48146 18.63713 7.936976 0.3865762 0.5468586 -1.230476 1.0878844 1.7740265 0.7718185 0.2977254 0.77181854 -1.19023134 0.08474167 0.095933333 0.1069667 0.01103333 0.350155563 0.009134908 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125319 E001 0.2214452 0.0378723757 1.000000e+00   17 44141838 44141905 68 + 0.001 0.081 7.205
ENSG00000125319 E002 1.5102910 0.0092399492 2.962272e-01 4.374311e-01 17 44141906 44141929 24 + 0.488 0.306 -1.024
ENSG00000125319 E003 1.5102910 0.0092399492 2.962272e-01 4.374311e-01 17 44141930 44141932 3 + 0.488 0.306 -1.024
ENSG00000125319 E004 3.2314312 0.0050210634 1.920725e-01 3.163448e-01 17 44141933 44141937 5 + 0.712 0.511 -0.886
ENSG00000125319 E005 4.0430304 0.0045771719 6.630904e-02 1.372317e-01 17 44141938 44141938 1 + 0.827 0.563 -1.104
ENSG00000125319 E006 41.0762557 0.0005606159 3.099852e-02 7.391398e-02 17 44141939 44142021 83 + 1.644 1.525 -0.408
ENSG00000125319 E007 65.0881279 0.0004496433 4.050816e-05 2.331435e-04 17 44142022 44142145 124 + 1.882 1.701 -0.613
ENSG00000125319 E008 49.1351947 0.0005450897 1.259333e-05 8.177160e-05 17 44145203 44145253 51 + 1.790 1.570 -0.749
ENSG00000125319 E009 0.4031496 0.0248171233 6.060580e-01 7.263103e-01 17 44147771 44147857 87 + 0.000 0.149 10.149
ENSG00000125319 E010 113.3539431 0.0004295843 8.606378e-10 1.290630e-08 17 44147858 44148126 269 + 2.137 1.932 -0.686
ENSG00000125319 E011 335.1901272 0.0002170997 8.410111e-03 2.481304e-02 17 44148127 44149027 901 + 2.492 2.447 -0.149
ENSG00000125319 E012 23.4165769 0.0119444101 5.934643e-02 1.255220e-01 17 44150961 44150963 3 + 1.161 1.361 0.702
ENSG00000125319 E013 58.7354912 0.0004890942 7.744291e-02 1.553529e-01 17 44150964 44151044 81 + 1.634 1.734 0.342
ENSG00000125319 E014 66.6530142 0.0003650513 2.322572e-02 5.821472e-02 17 44152637 44152777 141 + 1.669 1.790 0.409
ENSG00000125319 E015 8.5068030 0.0020662459 1.537746e-01 2.668023e-01 17 44154360 44154552 193 + 1.036 0.877 -0.594
ENSG00000125319 E016 7.1416742 0.0025313935 1.470500e-02 3.982472e-02 17 44154553 44154555 3 + 1.056 0.771 -1.084
ENSG00000125319 E017 98.2051779 0.0003390701 5.112622e-01 6.463214e-01 17 44154556 44154664 109 + 1.902 1.935 0.110
ENSG00000125319 E018 82.7928492 0.0003694323 2.600736e-04 1.217088e-03 17 44154853 44154938 86 + 1.715 1.891 0.594
ENSG00000125319 E019 113.1043351 0.0002772609 5.892973e-04 2.494316e-03 17 44155286 44155411 126 + 1.879 2.019 0.470
ENSG00000125319 E020 103.2329393 0.0044314196 1.249584e-01 2.270638e-01 17 44157833 44157941 109 + 1.885 1.968 0.277
ENSG00000125319 E021 289.8562976 0.0016883487 2.491636e-05 1.511559e-04 17 44161871 44162511 641 + 2.300 2.426 0.419