ENSG00000125266

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000643990 ENSG00000125266 HEK293_OSMI2_2hA HEK293_TMG_2hB EFNB2 protein_coding processed_transcript 11.01541 8.036119 11.00192 0.4021596 0.2922251 0.452701 6.473472 5.667768 5.312475 0.5456450 0.6306103 -0.09322655 0.5982375 0.7037333 0.4806333 -0.2231000 0.03642136 0.03642136 FALSE TRUE
ENST00000646441 ENSG00000125266 HEK293_OSMI2_2hA HEK293_TMG_2hB EFNB2 protein_coding protein_coding 11.01541 8.036119 11.00192 0.4021596 0.2922251 0.452701 4.263247 2.368352 5.339823 0.3625163 0.4140885 1.16952924 0.3805625 0.2962667 0.4880000 0.1917333 0.10502802 0.03642136 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125266 E001 0.00000       13 106487188 106487249 62 -      
ENSG00000125266 E002 12.14012 0.013583273 4.376007e-02 9.794908e-02 13 106489745 106489809 65 - 0.958 1.249 1.051
ENSG00000125266 E003 702.71586 0.009253193 3.067514e-08 3.445988e-07 13 106489810 106492410 2601 - 2.743 2.947 0.679
ENSG00000125266 E004 265.79714 0.010686276 1.107463e-03 4.328029e-03 13 106492411 106493428 1018 - 2.450 2.388 -0.208
ENSG00000125266 E005 67.08316 0.001235877 1.402685e-03 5.315541e-03 13 106494881 106494994 114 - 1.856 1.795 -0.207
ENSG00000125266 E006 55.74170 0.005788018 3.437637e-02 8.047674e-02 13 106495748 106495840 93 - 1.768 1.723 -0.153
ENSG00000125266 E007 79.97693 0.014566151 1.063584e-02 3.029789e-02 13 106512529 106512812 284 - 1.944 1.848 -0.323
ENSG00000125266 E008 0.00000       13 106516438 106516447 10 -      
ENSG00000125266 E009 56.97296 0.019253097 5.926014e-07 5.167859e-06 13 106534843 106535662 820 - 1.876 1.538 -1.144