ENSG00000125249

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000245304 ENSG00000125249 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP2A protein_coding protein_coding 8.043479 2.700396 13.76903 0.555646 0.7215517 2.345899 5.37140675 1.0582426 9.37418725 0.1507449 0.54013310 3.134993 0.63541667 0.4024667 0.682000000 0.27953333 0.0001455634 0.0001455634 FALSE TRUE
ENST00000476869 ENSG00000125249 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP2A protein_coding nonsense_mediated_decay 8.043479 2.700396 13.76903 0.555646 0.7215517 2.345899 0.09841041 0.1827219 0.03415687 0.1039495 0.03415687 -2.125811 0.02525833 0.0652000 0.002766667 -0.06243333 0.1305796546 0.0001455634 TRUE FALSE
MSTRG.8975.2 ENSG00000125249 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP2A protein_coding   8.043479 2.700396 13.76903 0.555646 0.7215517 2.345899 2.54829395 1.4453254 4.32354591 0.3445903 0.61698048 1.574206 0.33499167 0.5273667 0.312200000 -0.21516667 0.0166157922 0.0001455634 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125249 E001 0.9609246 0.0136968632 3.879865e-01 5.320975e-01 13 97432784 97433714 931 + 0.208 0.376 1.164
ENSG00000125249 E002 1.2554226 0.0624081368 6.610160e-01 7.695622e-01 13 97433715 97433755 41 + 0.284 0.376 0.580
ENSG00000125249 E003 24.5315212 0.0043620208 8.953225e-02 1.744143e-01 13 97434169 97434470 302 + 1.353 1.190 -0.572
ENSG00000125249 E004 29.0063941 0.0006917294 9.870325e-02 1.885116e-01 13 97434471 97434513 43 + 1.424 1.292 -0.458
ENSG00000125249 E005 30.8510177 0.0006583544 7.789921e-03 2.325120e-02 13 97434514 97434536 23 + 1.462 1.244 -0.760
ENSG00000125249 E006 93.6695173 0.0004396062 1.032321e-03 4.070138e-03 13 97434537 97434784 248 + 1.920 1.770 -0.509
ENSG00000125249 E007 0.3299976 0.0261153644 1.609820e-02   13 97441768 97441850 83 + 0.000 0.376 12.544
ENSG00000125249 E008 999.1209694 0.0002403369 1.905332e-06 1.489291e-05 13 97464205 97469128 4924 + 2.910 2.939 0.097