ENSG00000125149

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000569600 ENSG00000125149 HEK293_OSMI2_2hA HEK293_TMG_2hB PHAF1 protein_coding protein_coding 18.69709 17.75603 20.08034 0.4845727 0.3577971 0.1773807 10.660151 7.676242 13.1276604 0.7844726 0.1940516 0.77335804 0.5653458 0.4311000 0.6538333 0.2227333 5.069232e-05 5.069232e-05 FALSE TRUE
MSTRG.12800.1 ENSG00000125149 HEK293_OSMI2_2hA HEK293_TMG_2hB PHAF1 protein_coding   18.69709 17.75603 20.08034 0.4845727 0.3577971 0.1773807 4.788250 4.889466 4.6794618 0.7022218 0.4342247 -0.06320203 0.2593792 0.2772000 0.2325000 -0.0447000 7.433676e-01 5.069232e-05 FALSE TRUE
MSTRG.12800.6 ENSG00000125149 HEK293_OSMI2_2hA HEK293_TMG_2hB PHAF1 protein_coding   18.69709 17.75603 20.08034 0.4845727 0.3577971 0.1773807 1.236887 3.272598 0.6468473 0.2182316 0.3262541 -2.32120840 0.0676625 0.1839333 0.0327000 -0.1512333 1.748687e-01 5.069232e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000125149 E001 0.2944980 0.3446351566 2.419853e-01   16 67109908 67109928 21 + 0.225 0.000 -10.459
ENSG00000125149 E002 0.4460135 0.0281865389 6.399385e-02 1.333433e-01 16 67109929 67109940 12 + 0.306 0.000 -14.178
ENSG00000125149 E003 1.3380404 0.3032487107 9.448196e-03 2.740503e-02 16 67109941 67109949 9 + 0.610 0.000 -14.419
ENSG00000125149 E004 6.6492951 0.0024426313 1.049913e-03 4.132274e-03 16 67109950 67109957 8 + 1.050 0.675 -1.453
ENSG00000125149 E005 11.2371756 0.0016814478 1.392247e-01 2.469973e-01 16 67109958 67109961 4 + 1.155 1.039 -0.417
ENSG00000125149 E006 12.4475693 0.0019097758 3.614832e-01 5.058720e-01 16 67109962 67109962 1 + 1.165 1.107 -0.206
ENSG00000125149 E007 25.0065269 0.0039958271 2.043773e-02 5.241105e-02 16 67109963 67109989 27 + 1.491 1.350 -0.485
ENSG00000125149 E008 13.7784110 0.0016059509 3.602815e-02 8.364113e-02 16 67109990 67110004 15 + 1.256 1.097 -0.566
ENSG00000125149 E009 3.8968281 0.0043721515 4.347911e-01 5.770785e-01 16 67110005 67110116 112 + 0.735 0.646 -0.372
ENSG00000125149 E010 2.2704074 0.0126010075 8.138700e-01 8.820312e-01 16 67110117 67110118 2 + 0.483 0.542 0.284
ENSG00000125149 E011 37.0823163 0.0005819139 6.025015e-03 1.867783e-02 16 67110119 67110144 26 + 1.649 1.523 -0.431
ENSG00000125149 E012 39.2235845 0.0006153625 1.553684e-02 4.170609e-02 16 67110145 67110146 2 + 1.662 1.558 -0.356
ENSG00000125149 E013 82.4204980 0.0003461332 1.417222e-05 9.088980e-05 16 67110147 67110239 93 + 1.993 1.855 -0.465
ENSG00000125149 E014 0.1472490 0.0431290147 4.258310e-01   16 67120095 67120111 17 + 0.127 0.000 -12.393
ENSG00000125149 E015 97.9984200 0.0002890944 1.175925e-05 7.687834e-05 16 67120112 67120194 83 + 2.061 1.935 -0.421
ENSG00000125149 E016 110.1401876 0.0003259317 2.819950e-05 1.687987e-04 16 67125959 67126042 84 + 2.104 1.993 -0.373
ENSG00000125149 E017 79.6355730 0.0003750842 6.254459e-02 1.309284e-01 16 67131286 67131329 44 + 1.930 1.886 -0.149
ENSG00000125149 E018 1.7714650 0.4739688423 4.991051e-01 6.354519e-01 16 67131989 67132445 457 + 0.483 0.373 -0.588
ENSG00000125149 E019 97.4809804 0.0003727453 3.718789e-02 8.582729e-02 16 67132446 67132525 80 + 2.013 1.967 -0.155
ENSG00000125149 E020 1.6565104 0.0081651385 7.643380e-02 1.537955e-01 16 67132526 67132816 291 + 0.571 0.286 -1.547
ENSG00000125149 E021 105.1337002 0.0106989532 2.777299e-01 4.173449e-01 16 67132817 67132911 95 + 2.045 2.002 -0.144
ENSG00000125149 E022 1.3900112 0.0177784963 5.771880e-01 7.024749e-01 16 67132912 67132934 23 + 0.432 0.351 -0.455
ENSG00000125149 E023 132.1435941 0.0006288036 1.482577e-04 7.394618e-04 16 67134168 67134265 98 + 2.172 2.079 -0.308
ENSG00000125149 E024 4.3094158 0.0038025662 4.356706e-01 5.778693e-01 16 67134266 67134354 89 + 0.761 0.675 -0.352
ENSG00000125149 E025 148.3481493 0.0002527868 5.632451e-05 3.132857e-04 16 67134355 67134467 113 + 2.217 2.129 -0.292
ENSG00000125149 E026 0.1451727 0.0432423976 4.259223e-01   16 67134468 67134627 160 + 0.127 0.000 -12.391
ENSG00000125149 E027 1.1039192 0.0271135158 8.758297e-01 9.242660e-01 16 67134628 67134771 144 + 0.306 0.350 0.281
ENSG00000125149 E028 0.9555373 0.0154429671 1.354989e-01 2.418133e-01 16 67134772 67135192 421 + 0.127 0.407 2.188
ENSG00000125149 E029 0.9577023 0.0149216327 8.472447e-01 9.051183e-01 16 67135193 67136255 1063 + 0.306 0.286 -0.132
ENSG00000125149 E030 0.3686942 0.0314365588 9.118088e-01 9.480599e-01 16 67136256 67136310 55 + 0.127 0.118 -0.128
ENSG00000125149 E031 1.2575084 0.1083753540 1.320748e-02 3.639390e-02 16 67139634 67139983 350 + 0.000 0.502 13.717
ENSG00000125149 E032 156.4975323 0.0012685316 2.599868e-01 3.974734e-01 16 67139984 67140117 134 + 2.197 2.188 -0.029
ENSG00000125149 E033 126.4697559 0.0003004739 7.920562e-01 8.670057e-01 16 67140511 67140594 84 + 2.088 2.109 0.069
ENSG00000125149 E034 136.5265194 0.0016805873 9.970379e-01 1.000000e+00 16 67144294 67144376 83 + 2.118 2.145 0.094
ENSG00000125149 E035 121.2510639 0.0003180452 9.527181e-01 9.743816e-01 16 67144834 67144877 44 + 2.066 2.097 0.104
ENSG00000125149 E036 121.7765519 0.0004035855 8.223032e-01 8.879604e-01 16 67145376 67145419 44 + 2.075 2.097 0.074
ENSG00000125149 E037 131.7000539 0.0011012520 8.835938e-01 9.295443e-01 16 67145570 67145628 59 + 2.105 2.128 0.079
ENSG00000125149 E038 91.4675741 0.0020221257 9.233740e-01 9.557003e-01 16 67146278 67146283 6 + 1.947 1.971 0.079
ENSG00000125149 E039 147.1970652 0.0002404049 5.999821e-01 7.212899e-01 16 67146284 67146350 67 + 2.143 2.187 0.149
ENSG00000125149 E040 138.3546380 0.0011876896 5.409269e-01 6.719758e-01 16 67147045 67147094 50 + 2.114 2.165 0.169
ENSG00000125149 E041 259.6003733 0.0002322709 1.332389e-01 2.386303e-01 16 67147095 67147305 211 + 2.375 2.438 0.208
ENSG00000125149 E042 698.3206292 0.0029342759 1.330035e-13 3.714745e-12 16 67147306 67150437 3132 + 2.708 2.921 0.708