ENSG00000124613

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000244576 ENSG00000124613 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF391 protein_coding protein_coding 5.746676 5.131732 6.343823 0.1599884 0.1800509 0.3053705 1.8488927 2.11775006 1.7921195 0.08358643 0.32675637 -0.239634 0.3266917 0.4133 0.2802000 -0.1331000 1.567576e-01 2.356638e-08 FALSE TRUE
ENST00000461521 ENSG00000124613 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF391 protein_coding protein_coding 5.746676 5.131732 6.343823 0.1599884 0.1800509 0.3053705 1.7087574 0.17784909 2.9018757 0.09264612 0.39058857 3.954303 0.2816167 0.0338 0.4615000 0.4277000 7.065479e-04 2.356638e-08 FALSE TRUE
ENST00000482767 ENSG00000124613 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF391 protein_coding processed_transcript 5.746676 5.131732 6.343823 0.1599884 0.1800509 0.3053705 1.6397172 2.78737100 0.8873005 0.13166792 0.21976572 -1.640408 0.3024542 0.5433 0.1383667 -0.4049333 2.356638e-08 2.356638e-08   FALSE
MSTRG.27836.4 ENSG00000124613 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF391 protein_coding   5.746676 5.131732 6.343823 0.1599884 0.1800509 0.3053705 0.4793657 0.04876193 0.7544780 0.02905791 0.08201372 3.701521 0.0792000 0.0096 0.1185333 0.1089333 4.739917e-07 2.356638e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124613 E001 0.0000000       6 27372924 27374614 1691 +      
ENSG00000124613 E002 0.2966881 0.0272056545 3.913495e-01   6 27374615 27375137 523 + 0.176 0.001 -8.700
ENSG00000124613 E003 16.5977894 0.0011799574 3.474433e-13 9.147038e-12 6 27388759 27388824 66 + 0.874 1.494 2.222
ENSG00000124613 E004 23.7417528 0.0008431645 1.818107e-14 5.765564e-13 6 27388825 27388903 79 + 1.078 1.622 1.898
ENSG00000124613 E005 24.7448038 0.0007734009 1.806923e-08 2.123681e-07 6 27388904 27388991 88 + 1.202 1.585 1.325
ENSG00000124613 E006 27.5332596 0.0007034286 5.886221e-02 1.246830e-01 6 27388992 27389043 52 + 1.392 1.512 0.412
ENSG00000124613 E007 22.9616892 0.0008236301 3.146268e-01 4.573610e-01 6 27389044 27389075 32 + 1.341 1.409 0.238
ENSG00000124613 E008 10.3908186 0.0031606839 9.126348e-10 1.363210e-08 6 27390980 27391132 153 + 0.675 1.321 2.418
ENSG00000124613 E009 13.2652154 0.0018433703 3.099224e-09 4.192649e-08 6 27395017 27395095 79 + 1.321 0.644 -2.549
ENSG00000124613 E010 4.3158549 0.0038624585 1.593747e-05 1.011154e-04 6 27397457 27397576 120 + 0.902 0.154 -4.035
ENSG00000124613 E011 20.7503358 0.0009812005 4.448495e-08 4.847593e-07 6 27399442 27399550 109 + 1.468 0.997 -1.668
ENSG00000124613 E012 349.0290592 0.0003071982 3.548805e-12 7.955396e-11 6 27400293 27403908 3616 + 2.569 2.486 -0.274