ENSG00000124574

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000437104 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding retained_intron 11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.8670752 0.5822750 0.6589059 0.07305426 0.15320806 0.1755363 0.07447083 0.04486667 0.08476667 0.03990000 1.985037e-01 2.631589e-18 FALSE FALSE
ENST00000443426 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding processed_transcript 11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.9603833 0.8276251 0.6889283 0.07630710 0.06363610 -0.2611601 0.08557917 0.06396667 0.08830000 0.02433333 3.980598e-01 2.631589e-18 FALSE TRUE
ENST00000463024 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding retained_intron 11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.5891081 0.4872167 0.4076820 0.06748878 0.01194049 -0.2514699 0.05222083 0.03710000 0.05280000 0.01570000 4.645330e-01 2.631589e-18 FALSE TRUE
ENST00000469856 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding retained_intron 11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.4587918 0.2625140 0.7705400 0.26251400 0.02374221 1.5181422 0.04179167 0.01633333 0.10016667 0.08383333 8.588496e-02 2.631589e-18   FALSE
MSTRG.28326.10 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding   11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 3.7768415 3.8924717 2.8478177 0.92517752 0.33773874 -0.4494743 0.34200000 0.29383333 0.37223333 0.07840000 6.969818e-01 2.631589e-18 TRUE TRUE
MSTRG.28326.11 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding   11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 1.2753017 4.1556512 0.0000000 0.33720216 0.00000000 -8.7023982 0.10150000 0.32833333 0.00000000 -0.32833333 2.631589e-18 2.631589e-18 FALSE TRUE
MSTRG.28326.14 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding   11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.6419427 0.4934630 0.4227303 0.14102238 0.13870275 -0.2184176 0.05936667 0.04140000 0.05720000 0.01580000 8.363957e-01 2.631589e-18 TRUE TRUE
MSTRG.28326.15 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding   11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.5564385 0.5125070 0.4114794 0.33697022 0.22131446 -0.3099882 0.05007083 0.03416667 0.04873333 0.01456667 9.359056e-01 2.631589e-18 TRUE TRUE
MSTRG.28326.2 ENSG00000124574 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCC10 protein_coding   11.26087 13.07737 7.794699 1.596228 0.6180899 -0.7457602 0.5676209 0.6392770 0.3909316 0.35763276 0.21614529 -0.6954781 0.05087500 0.04376667 0.05206667 0.00830000 9.924899e-01 2.631589e-18 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124574 E001 0.0000000       6 43427366 43427389 24 +      
ENSG00000124574 E002 0.1817044 0.0393743835 1.000000e+00   6 43427541 43427547 7 + 0.001 0.086 7.269
ENSG00000124574 E003 0.1817044 0.0393743835 1.000000e+00   6 43427548 43427553 6 + 0.000 0.086 9.710
ENSG00000124574 E004 0.4815130 0.0220842450 9.383863e-01 9.653104e-01 6 43427554 43427555 2 + 0.172 0.157 -0.158
ENSG00000124574 E005 4.5717583 0.0066274495 2.426913e-01 3.773357e-01 6 43427556 43427574 19 + 0.802 0.631 -0.704
ENSG00000124574 E006 6.3369057 0.0181015198 2.202192e-01 3.506792e-01 6 43427575 43427578 4 + 0.918 0.750 -0.655
ENSG00000124574 E007 19.8185147 0.0009323433 1.601152e-01 2.752723e-01 6 43427579 43427699 121 + 1.340 1.223 -0.411
ENSG00000124574 E008 6.8452705 0.0057492458 9.616816e-01 9.798553e-01 6 43427700 43427757 58 + 0.834 0.839 0.018
ENSG00000124574 E009 0.7373013 0.2446532379 6.511967e-01 7.621237e-01 6 43427856 43427863 8 + 0.294 0.213 -0.619
ENSG00000124574 E010 1.9541264 0.0075974365 4.484759e-01 5.897587e-01 6 43427864 43427883 20 + 0.535 0.402 -0.671
ENSG00000124574 E011 8.6939687 0.0021588650 7.697018e-02 1.546432e-01 6 43427884 43427967 84 + 1.068 0.865 -0.756
ENSG00000124574 E012 38.5797582 0.0113997003 1.683995e-01 2.860971e-01 6 43427968 43428139 172 + 1.611 1.507 -0.356
ENSG00000124574 E013 3.7245698 0.0044724718 7.995875e-01 8.721706e-01 6 43430748 43430866 119 + 0.593 0.631 0.168
ENSG00000124574 E014 2.6969377 0.0085806514 7.091612e-01 8.066222e-01 6 43431751 43432141 391 + 0.469 0.532 0.306
ENSG00000124574 E015 49.8016697 0.0040715463 1.109597e-01 2.069606e-01 6 43432142 43432294 153 + 1.712 1.616 -0.326
ENSG00000124574 E016 172.2815129 0.0064620958 3.161370e-04 1.445286e-03 6 43432295 43433234 940 + 2.292 2.134 -0.527
ENSG00000124574 E017 59.2709516 0.0032406828 2.289353e-03 8.117674e-03 6 43433235 43433360 126 + 1.833 1.668 -0.558
ENSG00000124574 E018 88.9660862 0.0003732581 1.348893e-02 3.705432e-02 6 43434621 43434848 228 + 1.965 1.864 -0.342
ENSG00000124574 E019 10.5533000 0.0016352809 2.920336e-02 7.039596e-02 6 43434849 43435156 308 + 1.164 0.936 -0.833
ENSG00000124574 E020 49.8022302 0.0004551897 1.530356e-02 4.117559e-02 6 43435751 43435797 47 + 1.740 1.610 -0.441
ENSG00000124574 E021 64.9542629 0.0003911758 1.375986e-01 2.447389e-01 6 43435798 43435907 110 + 1.814 1.741 -0.245
ENSG00000124574 E022 2.9266025 0.0834902963 8.108071e-01 8.799703e-01 6 43436093 43436137 45 + 0.593 0.554 -0.179
ENSG00000124574 E023 60.7132271 0.0004074793 3.817908e-01 5.260720e-01 6 43436138 43436247 110 + 1.766 1.720 -0.155
ENSG00000124574 E024 60.6311124 0.0005130023 7.686929e-01 8.505181e-01 6 43437934 43438013 80 + 1.728 1.739 0.038
ENSG00000124574 E025 3.0592892 0.0056172116 8.782101e-01 9.257930e-01 6 43438014 43438623 610 + 0.535 0.559 0.110
ENSG00000124574 E026 63.1751903 0.0003850032 7.921408e-01 8.670613e-01 6 43438624 43438710 87 + 1.762 1.746 -0.054
ENSG00000124574 E027 64.2628653 0.0004398010 5.455558e-01 6.758692e-01 6 43438711 43438795 85 + 1.780 1.748 -0.108
ENSG00000124574 E028 60.6563564 0.0060498334 7.218232e-01 8.160653e-01 6 43441862 43441960 99 + 1.720 1.742 0.073
ENSG00000124574 E029 2.9497098 0.0053324189 2.301231e-03 8.154450e-03 6 43441961 43442061 101 + 0.834 0.363 -2.154
ENSG00000124574 E030 80.0969847 0.0004893130 7.376900e-02 1.495534e-01 6 43442970 43443159 190 + 1.790 1.868 0.260
ENSG00000124574 E031 27.8291463 0.0007514872 3.909237e-07 3.531006e-06 6 43443160 43443932 773 + 1.621 1.281 -1.172
ENSG00000124574 E032 63.1147277 0.0004367372 2.386739e-01 3.726086e-01 6 43443933 43444010 78 + 1.708 1.763 0.187
ENSG00000124574 E033 83.4192776 0.0003146925 1.159752e-01 2.142043e-01 6 43444159 43444353 195 + 1.820 1.885 0.218
ENSG00000124574 E034 63.3689215 0.0008242355 4.964369e-01 6.331031e-01 6 43444788 43444938 151 + 1.732 1.763 0.105
ENSG00000124574 E035 1.4948784 0.3211422312 8.343347e-02 1.649368e-01 6 43445100 43445124 25 + 0.645 0.211 -2.448
ENSG00000124574 E036 72.4222637 0.0004261618 6.085049e-01 7.282247e-01 6 43445125 43445314 190 + 1.830 1.804 -0.088
ENSG00000124574 E037 84.3773327 0.0018616444 2.242434e-01 3.555028e-01 6 43445599 43445814 216 + 1.833 1.888 0.186
ENSG00000124574 E038 66.6338747 0.0003982606 6.505562e-03 1.993626e-02 6 43445815 43445942 128 + 1.673 1.805 0.445
ENSG00000124574 E039 76.8047721 0.0052094219 3.719010e-02 8.583031e-02 6 43446277 43446446 170 + 1.751 1.868 0.393
ENSG00000124574 E040 4.5518757 0.0080820787 5.277686e-01 6.607685e-01 6 43446855 43447247 393 + 0.768 0.673 -0.391
ENSG00000124574 E041 94.6389622 0.0004876071 2.474571e-02 6.135351e-02 6 43447248 43447408 161 + 1.854 1.944 0.303
ENSG00000124574 E042 3.3224524 0.0068454680 6.057682e-01 7.260902e-01 6 43447409 43447437 29 + 0.643 0.559 -0.376
ENSG00000124574 E043 137.5634077 0.0002727214 1.885895e-02 4.902401e-02 6 43447684 43447937 254 + 2.029 2.105 0.255
ENSG00000124574 E044 2.4669477 0.1466490271 5.575530e-01 6.859548e-01 6 43447993 43448106 114 + 0.593 0.446 -0.699
ENSG00000124574 E045 120.3826736 0.0004406175 1.207171e-03 4.664769e-03 6 43448881 43449026 146 + 1.942 2.059 0.392
ENSG00000124574 E046 115.1225593 0.0008078490 1.857005e-02 4.838387e-02 6 43449107 43449204 98 + 1.944 2.032 0.296
ENSG00000124574 E047 119.1041890 0.0019070556 2.808215e-02 6.817641e-02 6 43449422 43449534 113 + 1.958 2.048 0.300
ENSG00000124574 E048 169.4837831 0.0051774212 1.640824e-04 8.096505e-04 6 43449929 43450324 396 + 2.046 2.220 0.580
ENSG00000124574 E049 14.4293371 0.0688935903 9.828659e-02 1.879186e-01 6 43450325 43451895 1571 + 0.942 1.195 0.922
ENSG00000124574 E050 11.0109573 0.0021930024 1.818433e-04 8.858461e-04 6 43451896 43451994 99 + 1.267 0.890 -1.371
ENSG00000124574 E051 1.4027828 0.0336673626 7.074069e-01 8.053369e-01 6 43451995 43452531 537 + 0.390 0.321 -0.414
ENSG00000124574 E052 3.7587396 0.0938068453 5.338562e-01 6.659775e-01 6 43453016 43453263 248 + 0.730 0.621 -0.461
ENSG00000124574 E053 8.2568509 0.0123581605 1.155884e-06 9.471398e-06 6 43453976 43454037 62 + 1.255 0.673 -2.198
ENSG00000124574 E054 0.9222279 0.0136182854 3.430192e-01 4.870631e-01 6 43454184 43454230 47 + 0.390 0.218 -1.158
ENSG00000124574 E055 16.2834612 0.0114355121 2.964685e-05 1.766530e-04 6 43454624 43455197 574 + 1.435 1.028 -1.442