ENSG00000124535

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380764 ENSG00000124535 HEK293_OSMI2_2hA HEK293_TMG_2hB WRNIP1 protein_coding protein_coding 32.38002 38.21835 31.25311 2.886522 0.9487034 -0.2901813 1.193617 4.705828 0.000000 1.434799 0.0000000 -8.8813674 0.03391667 0.11976667 0.0000000 -0.1197667 4.363095e-14 4.363095e-14 FALSE TRUE
ENST00000380769 ENSG00000124535 HEK293_OSMI2_2hA HEK293_TMG_2hB WRNIP1 protein_coding protein_coding 32.38002 38.21835 31.25311 2.886522 0.9487034 -0.2901813 20.483566 25.202687 16.696307 2.458156 0.9291752 -0.5937571 0.63839167 0.65740000 0.5337333 -0.1236667 3.190376e-03 4.363095e-14 FALSE TRUE
ENST00000380773 ENSG00000124535 HEK293_OSMI2_2hA HEK293_TMG_2hB WRNIP1 protein_coding protein_coding 32.38002 38.21835 31.25311 2.886522 0.9487034 -0.2901813 5.329390 3.992916 7.277695 1.238409 1.1124520 0.8644112 0.16485833 0.10326667 0.2311333 0.1278667 1.218052e-01 4.363095e-14 FALSE TRUE
ENST00000618555 ENSG00000124535 HEK293_OSMI2_2hA HEK293_TMG_2hB WRNIP1 protein_coding protein_coding 32.38002 38.21835 31.25311 2.886522 0.9487034 -0.2901813 4.390571 2.573184 6.174529 2.090660 0.9675065 1.2595135 0.13263333 0.07306667 0.1996333 0.1265667 4.292160e-01 4.363095e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124535 E001 5.785279 0.0028125458 6.783240e-02 1.397706e-01 6 2765393 2765413 21 + 0.954 0.713 -0.941
ENSG00000124535 E002 7.004284 0.0024159412 6.606602e-03 2.020340e-02 6 2765414 2765431 18 + 1.067 0.736 -1.263
ENSG00000124535 E003 184.712930 0.0169235460 3.841652e-06 2.805989e-05 6 2765432 2766051 620 + 2.432 2.097 -1.121
ENSG00000124535 E004 409.092426 0.0027867109 1.087269e-09 1.601585e-08 6 2766052 2766444 393 + 2.714 2.513 -0.670
ENSG00000124535 E005 320.213361 0.0001526007 1.109416e-05 7.295479e-05 6 2768691 2768772 82 + 2.558 2.457 -0.334
ENSG00000124535 E006 396.379891 0.0026691952 2.364233e-01 3.700240e-01 6 2768773 2768882 110 + 2.620 2.568 -0.171
ENSG00000124535 E007 417.152418 0.0012331698 1.231440e-01 2.244472e-01 6 2770120 2770194 75 + 2.642 2.592 -0.167
ENSG00000124535 E008 613.585640 0.0001368895 3.965012e-02 9.037921e-02 6 2770195 2770361 167 + 2.805 2.762 -0.143
ENSG00000124535 E009 721.468582 0.0001497596 5.497517e-02 1.179475e-01 6 2779263 2779492 230 + 2.841 2.854 0.041
ENSG00000124535 E010 546.440732 0.0003796555 2.965464e-02 7.128697e-02 6 2783406 2783561 156 + 2.714 2.738 0.078
ENSG00000124535 E011 402.978564 0.0004145403 1.585611e-03 5.912243e-03 6 2784324 2784403 80 + 2.566 2.616 0.164
ENSG00000124535 E012 1391.310839 0.0002302393 1.503688e-29 2.424418e-27 6 2785007 2786952 1946 + 3.068 3.169 0.335