ENSG00000124459

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000269973 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding protein_coding 5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 1.0755585 0.00000000 2.4247139 0.00000000 0.2321822 7.9276085 0.14795417 0.00000000 0.28193333 0.28193333 6.337993e-16 6.337993e-16 FALSE TRUE
ENST00000589703 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding protein_coding 5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 0.1890714 0.00000000 0.5001645 0.00000000 0.5001645 5.6728905 0.02711667 0.00000000 0.05940000 0.05940000 1.000000e+00 6.337993e-16 FALSE TRUE
ENST00000615985 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding protein_coding 5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 1.4115180 1.40288177 0.8603522 0.04804074 0.4912078 -0.6989695 0.31011250 0.52070000 0.09816667 -0.42253333 1.257269e-01 6.337993e-16 FALSE TRUE
MSTRG.17268.1 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding   5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 0.5143097 0.20892875 0.8385811 0.13723769 0.3489407 1.9545911 0.07555417 0.07356667 0.09823333 0.02466667 8.488297e-01 6.337993e-16 FALSE TRUE
MSTRG.17268.2 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding   5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 0.8810820 0.68871910 1.1604590 0.17096873 0.6229762 0.7442899 0.16457917 0.26083333 0.13296667 -0.12786667 6.785675e-01 6.337993e-16 FALSE TRUE
MSTRG.17268.4 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding   5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 0.4467495 0.18688542 0.7475773 0.18688542 0.4651488 1.9440368 0.07377917 0.06656667 0.09006667 0.02350000 7.850361e-01 6.337993e-16 FALSE TRUE
MSTRG.17268.7 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding   5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 0.3277709 0.00000000 0.6988904 0.00000000 0.1118958 6.1474907 0.04763750 0.00000000 0.08196667 0.08196667 3.263887e-05 6.337993e-16 FALSE TRUE
MSTRG.17268.9 ENSG00000124459 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF45 protein_coding   5.789446 2.703561 8.576838 0.1258979 0.244101 1.661939 0.3311827 0.06780993 0.8930837 0.06780993 0.3405100 3.5368334 0.04528750 0.02416667 0.10376667 0.07960000 3.573607e-01 6.337993e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124459 E001 2.8473374 0.1342701352 1.943433e-01 3.192079e-01 19 43912511 43912517 7 - 0.456 0.741 1.278
ENSG00000124459 E002 6.2281971 0.0026236576 2.925780e-02 7.050185e-02 19 43912518 43912623 106 - 0.722 1.006 1.098
ENSG00000124459 E003 4.0181545 0.0039123261 1.012176e-01 1.923324e-01 19 43912624 43912630 7 - 0.578 0.827 1.037
ENSG00000124459 E004 7.5254478 0.0054274231 1.706388e-03 6.302175e-03 19 43912631 43912680 50 - 0.751 1.132 1.435
ENSG00000124459 E005 12.1553994 0.0244417294 2.380462e-03 8.399387e-03 19 43912681 43912712 32 - 0.944 1.308 1.311
ENSG00000124459 E006 65.3518631 0.0018601077 7.614760e-13 1.905168e-11 19 43912713 43913128 416 - 1.655 1.996 1.151
ENSG00000124459 E007 105.3660708 0.0035444865 1.321567e-03 5.048841e-03 19 43913129 43913624 496 - 1.935 2.080 0.486
ENSG00000124459 E008 62.7082973 0.0004468176 4.639476e-01 6.037055e-01 19 43913625 43913792 168 - 1.749 1.793 0.148
ENSG00000124459 E009 188.0806383 0.0002957038 3.760169e-01 5.203985e-01 19 43913793 43914940 1148 - 2.237 2.223 -0.048
ENSG00000124459 E010 45.2626994 0.0007394259 2.914719e-01 4.323435e-01 19 43914941 43915200 260 - 1.635 1.581 -0.182
ENSG00000124459 E011 31.1706390 0.0007587732 5.029836e-01 6.389728e-01 19 43918870 43918962 93 - 1.474 1.436 -0.131
ENSG00000124459 E012 36.7781135 0.0006304889 3.621548e-02 8.399395e-02 19 43919573 43919699 127 - 1.566 1.435 -0.447
ENSG00000124459 E013 3.3041399 0.0236630479 1.335368e-02 3.674385e-02 19 43919700 43919762 63 - 0.690 0.196 -2.771
ENSG00000124459 E014 22.6867732 0.0008717573 1.204345e-03 4.654987e-03 19 43922171 43922217 47 - 1.393 1.114 -0.986
ENSG00000124459 E015 0.3030308 0.4407300045 8.985860e-01   19 43922218 43922287 70 - 0.138 0.000 -9.891
ENSG00000124459 E016 29.4877001 0.0006596928 1.506532e-03 5.657245e-03 19 43924238 43924310 73 - 1.495 1.258 -0.820
ENSG00000124459 E017 0.1515154 0.0433597057 1.000000e+00   19 43924311 43924312 2 - 0.074 0.000 -9.870
ENSG00000124459 E018 42.3459382 0.0004949755 9.836492e-02 1.880285e-01 19 43924402 43924561 160 - 1.617 1.526 -0.314
ENSG00000124459 E019 33.4560201 0.0006521994 5.711379e-02 1.216596e-01 19 43925325 43925458 134 - 1.529 1.407 -0.420
ENSG00000124459 E020 3.8781681 0.0042237124 4.333437e-01 5.757464e-01 19 43929918 43929956 39 - 0.618 0.745 0.533
ENSG00000124459 E021 4.8662775 0.0033903880 6.887393e-01 7.907902e-01 19 43929957 43929997 41 - 0.751 0.698 -0.216
ENSG00000124459 E022 3.0990497 0.0049529209 9.930027e-01 9.997523e-01 19 43929998 43930000 3 - 0.578 0.586 0.036
ENSG00000124459 E023 14.9365144 0.0161183663 9.981892e-01 1.000000e+00 19 43931247 43931365 119 - 1.162 1.166 0.015
ENSG00000124459 E024 0.8899506 0.0139190784 1.317930e-01 2.366180e-01 19 43932403 43932603 201 - 0.325 0.000 -12.545
ENSG00000124459 E025 8.8372817 0.0018918210 2.847481e-01 4.249955e-01 19 43932604 43932607 4 - 0.988 0.862 -0.474
ENSG00000124459 E026 22.1013737 0.0012463277 2.771616e-02 6.745387e-02 19 43932608 43932690 83 - 1.367 1.184 -0.642
ENSG00000124459 E027 38.8347785 0.0005730679 3.115481e-05 1.846741e-04 19 43934426 43934694 269 - 1.621 1.345 -0.951
ENSG00000124459 E028 2.2980596 0.0140927872 6.562323e-01 7.659113e-01 19 43934695 43934727 33 - 0.509 0.433 -0.380
ENSG00000124459 E029 26.8881894 0.0056636764 4.538186e-02 1.008961e-01 19 43934924 43935229 306 - 1.439 1.273 -0.580
ENSG00000124459 E030 5.5910822 0.0037952982 4.460613e-01 5.875950e-01 19 43935230 43935294 65 - 0.805 0.699 -0.428