ENSG00000124422

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261497 ENSG00000124422 HEK293_OSMI2_2hA HEK293_TMG_2hB USP22 protein_coding protein_coding 119.1598 149.5772 97.65022 9.123425 0.5556633 -0.6151434 73.57304 61.36815 86.11106 13.455897 1.854812 0.488641 0.6386542 0.4028333 0.8818333 0.4790000 1.909470e-09 9.323925e-40 FALSE TRUE
ENST00000537526 ENSG00000124422 HEK293_OSMI2_2hA HEK293_TMG_2hB USP22 protein_coding protein_coding 119.1598 149.5772 97.65022 9.123425 0.5556633 -0.6151434 37.08006 84.89300 0.00000 5.585193 0.000000 -13.051600 0.2811792 0.5756333 0.0000000 -0.5756333 9.323925e-40 9.323925e-40 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124422 E001 4.481018e-01 0.1030291081 8.774693e-01 9.252738e-01 17 20989928 20989989 62 - 0.133 0.201 0.717
ENSG00000124422 E002 1.280649e+01 0.0013704406 5.249825e-09 6.823764e-08 17 20999596 20999596 1 - 0.497 1.314 3.194
ENSG00000124422 E003 1.097746e+04 0.0052662215 4.322380e-20 2.773922e-18 17 20999597 21002374 2778 - 3.818 4.136 1.057
ENSG00000124422 E004 1.625574e+03 0.0008961957 8.628625e-01 9.155747e-01 17 21002375 21002842 468 - 3.130 3.247 0.389
ENSG00000124422 E005 5.337401e+02 0.0007157218 2.038670e-01 3.310391e-01 17 21002843 21002971 129 - 2.663 2.756 0.309
ENSG00000124422 E006 4.810399e+02 0.0005914528 7.862248e-01 8.630973e-01 17 21002972 21003073 102 - 2.604 2.717 0.376
ENSG00000124422 E007 4.329089e+02 0.0016695703 5.061276e-04 2.183259e-03 17 21004202 21004219 18 - 2.613 2.641 0.094
ENSG00000124422 E008 8.906224e+02 0.0004781077 2.592585e-11 5.045274e-10 17 21004220 21004351 132 - 2.934 2.947 0.045
ENSG00000124422 E009 7.431509e+02 0.0010475078 5.651297e-10 8.772708e-09 17 21004928 21004990 63 - 2.867 2.860 -0.023
ENSG00000124422 E010 1.137428e+01 0.0029719659 2.163965e-01 3.460747e-01 17 21004991 21005150 160 - 1.094 1.079 -0.056
ENSG00000124422 E011 8.791192e+00 0.0023861242 3.074959e-05 1.824891e-04 17 21006508 21006895 388 - 1.152 0.802 -1.304
ENSG00000124422 E012 8.281031e+02 0.0017987902 3.261660e-07 2.995064e-06 17 21006896 21006987 92 - 2.911 2.909 -0.007
ENSG00000124422 E013 9.284823e+02 0.0012318496 4.409913e-08 4.806932e-07 17 21007870 21007996 127 - 2.955 2.963 0.026
ENSG00000124422 E014 7.486966e+02 0.0008804764 1.231670e-13 3.454678e-12 17 21011151 21011229 79 - 2.881 2.857 -0.079
ENSG00000124422 E015 7.372547e+02 0.0017964439 4.692512e-10 7.381736e-09 17 21011230 21011309 80 - 2.878 2.847 -0.101
ENSG00000124422 E016 1.267676e+01 0.0013984874 1.776155e-04 8.680180e-04 17 21011310 21011614 305 - 1.250 1.009 -0.865
ENSG00000124422 E017 8.304268e+02 0.0014547346 1.136261e-12 2.761529e-11 17 21012830 21012935 106 - 2.933 2.896 -0.122
ENSG00000124422 E018 9.809826e+00 0.0413488148 8.324525e-02 1.646489e-01 17 21015651 21015751 101 - 1.095 0.971 -0.454
ENSG00000124422 E019 8.515873e+02 0.0004282454 1.700177e-30 2.971341e-28 17 21015752 21015899 148 - 2.956 2.896 -0.200
ENSG00000124422 E020 5.366842e+02 0.0019576789 1.810102e-09 2.555511e-08 17 21017942 21017995 54 - 2.744 2.706 -0.128
ENSG00000124422 E021 7.198994e+02 0.0025597120 1.838957e-07 1.774938e-06 17 21017996 21018111 116 - 2.863 2.840 -0.078
ENSG00000124422 E022 0.000000e+00       17 21018202 21018341 140 -      
ENSG00000124422 E023 7.481472e+02 0.0016712349 1.339716e-13 3.739026e-12 17 21019084 21019185 102 - 2.898 2.844 -0.179
ENSG00000124422 E024 7.239439e+02 0.0013961442 1.323016e-21 1.010651e-19 17 21021113 21021205 93 - 2.904 2.812 -0.308
ENSG00000124422 E025 4.600093e+02 0.0012969688 4.940230e-22 3.954000e-20 17 21021206 21021226 21 - 2.718 2.604 -0.377
ENSG00000124422 E026 6.812998e+02 0.0031233932 2.311137e-18 1.217231e-16 17 21028542 21028674 133 - 2.905 2.762 -0.474
ENSG00000124422 E027 2.935788e+00 0.1351709716 9.017858e-01 9.414121e-01 17 21042220 21042292 73 - 0.544 0.606 0.281
ENSG00000124422 E028 5.083411e+02 0.0103298305 5.136326e-15 1.763431e-13 17 21042665 21043032 368 - 2.843 2.562 -0.934
ENSG00000124422 E029 2.791674e+00 0.0078823337 8.425804e-01 9.019037e-01 17 21043321 21043625 305 - 0.544 0.599 0.249
ENSG00000124422 E030 5.085815e-01 0.1777864308 8.889652e-01 9.329879e-01 17 21043626 21043760 135 - 0.132 0.203 0.742