ENSG00000124228

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000618172 ENSG00000124228 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX27 protein_coding protein_coding 56.98817 64.68109 54.29139 1.42832 0.4958 -0.2525777 40.832480 50.737680 33.454784 2.771970 1.2389261 -0.6006982 0.70924167 0.78356667 0.61593333 -0.167633333 0.004665514 0.004665514 FALSE TRUE
MSTRG.20832.1 ENSG00000124228 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX27 protein_coding   56.98817 64.68109 54.29139 1.42832 0.4958 -0.2525777 3.376013 5.295293 4.113242 0.731128 0.3077580 -0.3636533 0.05825417 0.08243333 0.07566667 -0.006766667 0.917624277 0.004665514 FALSE TRUE
MSTRG.20832.2 ENSG00000124228 HEK293_OSMI2_2hA HEK293_TMG_2hB DDX27 protein_coding   56.98817 64.68109 54.29139 1.42832 0.4958 -0.2525777 7.656614 4.866314 12.176707 1.617503 0.8307692 1.3214453 0.13890833 0.07573333 0.22456667 0.148833333 0.021834140 0.004665514 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124228 E001 1.9478854 0.0077627671 2.548132e-01 3.915960e-01 20 49219324 49219346 23 + 0.565 0.378 -0.945
ENSG00000124228 E002 4.9684700 0.0033788075 3.120129e-02 7.429227e-02 20 49219347 49219373 27 + 0.917 0.636 -1.123
ENSG00000124228 E003 47.6844331 0.0057691762 9.908069e-02 1.890686e-01 20 49219374 49219415 42 + 1.730 1.631 -0.336
ENSG00000124228 E004 72.6740731 0.0049665436 3.452794e-04 1.561075e-03 20 49219416 49219431 16 + 1.958 1.771 -0.631
ENSG00000124228 E005 309.9222987 0.0027266533 2.110031e-08 2.449081e-07 20 49219432 49219541 110 + 2.579 2.403 -0.586
ENSG00000124228 E006 495.0648772 0.0020633266 3.261074e-09 4.395160e-08 20 49221452 49221598 147 + 2.770 2.617 -0.511
ENSG00000124228 E007 373.2751492 0.0008902725 7.586511e-07 6.468526e-06 20 49222957 49223016 60 + 2.624 2.515 -0.361
ENSG00000124228 E008 602.5610865 0.0003543512 4.324128e-13 1.124956e-11 20 49223268 49223433 166 + 2.835 2.722 -0.377
ENSG00000124228 E009 0.9148515 0.0230246785 4.092286e-02 9.274135e-02 20 49224775 49224944 170 + 0.000 0.378 10.649
ENSG00000124228 E010 281.4184919 0.0001768932 2.654352e-12 6.081992e-11 20 49224945 49224953 9 + 2.523 2.379 -0.479
ENSG00000124228 E011 333.2677868 0.0002701909 6.865771e-09 8.727096e-08 20 49224954 49224991 38 + 2.578 2.466 -0.375
ENSG00000124228 E012 414.0846126 0.0015279404 1.753829e-03 6.452358e-03 20 49225113 49225199 87 + 2.650 2.576 -0.247
ENSG00000124228 E013 361.6183679 0.0027433256 1.954611e-01 3.205353e-01 20 49226430 49226535 106 + 2.568 2.533 -0.119
ENSG00000124228 E014 398.9352683 0.0015093917 4.710761e-01 6.102234e-01 20 49228715 49228888 174 + 2.596 2.582 -0.046
ENSG00000124228 E015 427.3146550 0.0001539510 2.551020e-04 1.196541e-03 20 49230199 49230349 151 + 2.654 2.596 -0.194
ENSG00000124228 E016 39.7051954 0.0007118517 3.261096e-02 7.701871e-02 20 49231023 49231115 93 + 1.660 1.546 -0.388
ENSG00000124228 E017 437.3461015 0.0001913138 9.056219e-05 4.776773e-04 20 49233306 49233405 100 + 2.667 2.604 -0.210
ENSG00000124228 E018 487.0276254 0.0001248073 5.344015e-02 1.152729e-01 20 49233568 49233709 142 + 2.692 2.667 -0.084
ENSG00000124228 E019 9.0219256 0.0031376914 2.380547e-03 8.399529e-03 20 49233710 49233837 128 + 1.169 0.851 -1.172
ENSG00000124228 E020 54.1600071 0.0056241709 4.206687e-04 1.853661e-03 20 49233838 49234934 1097 + 1.841 1.636 -0.692
ENSG00000124228 E021 506.3872611 0.0012137789 8.014576e-01 8.735043e-01 20 49234935 49235088 154 + 2.693 2.693 0.000
ENSG00000124228 E022 96.2150312 0.0042001761 3.537096e-07 3.223457e-06 20 49235089 49236149 1061 + 2.105 1.866 -0.802
ENSG00000124228 E023 357.7640289 0.0001911418 2.866751e-01 4.270976e-01 20 49236150 49236231 82 + 2.525 2.552 0.093
ENSG00000124228 E024 6.4002202 0.0026867038 5.812030e-01 7.057791e-01 20 49236232 49236332 101 + 0.896 0.834 -0.238
ENSG00000124228 E025 583.9736123 0.0001434570 1.332032e-01 2.385877e-01 20 49236333 49236510 178 + 2.736 2.766 0.101
ENSG00000124228 E026 523.0008780 0.0006975889 1.660589e-02 4.407246e-02 20 49238949 49239055 107 + 2.672 2.727 0.183
ENSG00000124228 E027 541.2994721 0.0007804482 3.718547e-05 2.159685e-04 20 49239236 49239338 103 + 2.663 2.754 0.303
ENSG00000124228 E028 550.0158934 0.0017039095 8.364980e-09 1.045813e-07 20 49241893 49241987 95 + 2.628 2.779 0.502
ENSG00000124228 E029 632.0584053 0.0004432963 7.469876e-22 5.845293e-20 20 49242083 49242206 124 + 2.672 2.848 0.585
ENSG00000124228 E030 552.3886877 0.0024408580 6.181226e-09 7.932629e-08 20 49242594 49242681 88 + 2.615 2.788 0.574
ENSG00000124228 E031 421.3750960 0.0020637027 7.049668e-09 8.941741e-08 20 49243629 49243703 75 + 2.499 2.671 0.574
ENSG00000124228 E032 11.4844975 0.0019404729 8.532916e-01 9.091824e-01 20 49243704 49243815 112 + 1.068 1.093 0.090
ENSG00000124228 E033 430.4699719 0.0012243682 2.188737e-18 1.155013e-16 20 49243816 49243973 158 + 2.471 2.695 0.745
ENSG00000124228 E034 138.3570680 0.0017270510 1.357476e-13 3.785248e-12 20 49243974 49244077 104 + 1.929 2.223 0.985
ENSG00000124228 E035 1.3232880 0.2959111147 7.949363e-01 8.690286e-01 20 49244502 49244555 54 + 0.330 0.376 0.275