ENSG00000124224

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000244070 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.55718404 0.80995133 0.4826212 0.10292139 0.281881316 -0.7350597 0.21644583 0.35333333 0.14650000 -0.206833333 0.455083234 0.001275049 FALSE  
ENST00000473210 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.07967519 0.00000000 0.2265723 0.00000000 0.060075108 4.5642093 0.02722917 0.00000000 0.06416667 0.064166667 0.001275049 0.001275049    
ENST00000495058 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene retained_intron 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.13558614 0.05331249 0.1206562 0.01173032 0.003951697 1.0452137 0.05360833 0.02330000 0.03493333 0.011633333 0.801487741 0.001275049 TRUE  
ENST00000497138 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.69350844 0.29430577 1.0846210 0.16984457 0.180962265 1.8468378 0.24888750 0.11976667 0.30936667 0.189600000 0.295030353 0.001275049 TRUE  
ENST00000606437 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene retained_intron 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.02306862 0.18454895 0.0000000 0.18454895 0.000000000 -4.2820613 0.00798750 0.06390000 0.00000000 -0.063900000 0.705032424 0.001275049    
ENST00000650934 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.29209132 0.21451654 0.2862128 0.12425632 0.162142259 0.3998121 0.12872917 0.09623333 0.08666667 -0.009566667 1.000000000 0.001275049 TRUE  
ENST00000686997 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.05242603 0.03308981 0.2565300 0.03308981 0.140087612 2.6288791 0.01490000 0.01446667 0.07040000 0.055933333 0.614667632 0.001275049 TRUE  
ENST00000691176 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.16605600 0.03754144 0.3250931 0.03754144 0.138072435 2.8173046 0.06651667 0.01873333 0.09023333 0.071500000 0.301322204 0.001275049 TRUE  
ENST00000691692 ENSG00000124224 HEK293_OSMI2_2hA HEK293_TMG_2hB   transcribed_unprocessed_pseudogene processed_transcript 2.601807 2.392604 3.478411 0.2609721 0.1546074 0.5379711 0.28146222 0.55313804 0.3252111 0.17546873 0.093889966 -0.7484188 0.11587083 0.22100000 0.09316667 -0.127833333 0.173354061 0.001275049 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124224 E001 0.0000000       20 58228940 58228946 7 -      
ENSG00000124224 E002 70.3512090 0.0018674891 7.482401e-03 0.0224674816 20 58228947 58231788 2842 - 1.899 1.779 -0.403
ENSG00000124224 E003 12.2613961 0.0015166497 6.524714e-03 0.0199868617 20 58231789 58231927 139 - 1.223 0.945 -1.006
ENSG00000124224 E004 9.1354643 0.0020851182 2.531029e-02 0.0625147602 20 58232688 58232776 89 - 1.091 0.830 -0.974
ENSG00000124224 E005 5.5141392 0.0034185639 4.982050e-01 0.6347026023 20 58232777 58232803 27 - 0.840 0.743 -0.386
ENSG00000124224 E006 5.8450791 0.0030027190 4.346585e-01 0.5769745057 20 58232804 58232829 26 - 0.789 0.880 0.355
ENSG00000124224 E007 6.3791227 0.0102809716 4.893637e-01 0.6268065488 20 58232830 58232922 93 - 0.900 0.803 -0.378
ENSG00000124224 E008 6.1524938 0.0125559130 2.293238e-01 0.3616263142 20 58235240 58235276 37 - 0.914 0.743 -0.671
ENSG00000124224 E009 9.2086676 0.0037322554 1.027021e-01 0.1946224209 20 58235277 58235374 98 - 1.072 0.880 -0.714
ENSG00000124224 E010 0.7427016 0.0156477559 5.797785e-02 0.1231622078 20 58235375 58235887 513 - 0.359 0.000 -12.644
ENSG00000124224 E011 9.0841825 0.0019458589 3.202786e-02 0.0759121413 20 58236154 58236274 121 - 1.082 0.830 -0.941
ENSG00000124224 E012 1.0966340 0.1400793329 2.376208e-01 0.3713991649 20 58237919 58238210 292 - 0.405 0.151 -1.889
ENSG00000124224 E013 6.0132469 0.0030992780 6.259513e-02 0.1310225759 20 58238211 58238311 101 - 0.928 0.673 -1.010
ENSG00000124224 E014 7.0135976 0.0673111446 2.176713e-01 0.3475932799 20 58239241 58239402 162 - 0.977 0.773 -0.784
ENSG00000124224 E015 0.0000000       20 58239504 58239504 1 -      
ENSG00000124224 E016 0.0000000       20 58239505 58239505 1 -      
ENSG00000124224 E017 1.8038441 0.1021026659 5.109000e-01 0.6460489818 20 58239506 58239659 154 - 0.359 0.493 0.717
ENSG00000124224 E018 6.2344751 0.0481597507 7.292520e-01 0.8214735646 20 58239660 58239754 95 - 0.871 0.808 -0.244
ENSG00000124224 E019 6.5099727 0.0292544697 3.328707e-01 0.4765428574 20 58239755 58239845 91 - 0.927 0.775 -0.588
ENSG00000124224 E020 0.0000000       20 58239846 58239987 142 -      
ENSG00000124224 E021 5.5793403 0.0437555816 1.014068e-01 0.1926070706 20 58240515 58240610 96 - 0.913 0.634 -1.123
ENSG00000124224 E022 0.5911862 0.0185101716 1.032926e-01 0.1954878739 20 58240611 58240710 100 - 0.307 0.000 -12.340
ENSG00000124224 E023 0.1472490 0.0433210371 7.671924e-01   20 58242370 58243511 1142 - 0.099 0.000 -10.401
ENSG00000124224 E024 7.2893211 0.0025909034 7.250887e-02 0.1475275862 20 58243512 58243705 194 - 1.000 0.774 -0.867
ENSG00000124224 E025 0.1482932 0.0413944080 3.044397e-01   20 58245746 58245782 37 - 0.000 0.149 10.914
ENSG00000124224 E026 20.3545606 0.0009241053 1.332626e-01 0.2386602224 20 58245783 58246252 470 - 1.279 1.381 0.355
ENSG00000124224 E027 4.3941365 0.0037572734 8.907581e-01 0.9342224573 20 58246253 58246926 674 - 0.730 0.742 0.048
ENSG00000124224 E028 1.0309706 0.0145023744 1.962274e-02 0.0506791749 20 58246927 58247055 129 - 0.447 0.000 -13.098
ENSG00000124224 E029 7.0613351 0.0034570933 1.963767e-02 0.0507115014 20 58247056 58247321 266 - 0.770 1.036 1.014
ENSG00000124224 E030 17.9207587 0.0012031437 6.163585e-05 0.0003394537 20 58247322 58247506 185 - 1.108 1.417 1.085
ENSG00000124224 E031 0.8825612 0.1755502938 9.332994e-01 0.9619736997 20 58247620 58248114 495 - 0.248 0.265 0.128
ENSG00000124224 E032 15.0692843 0.0017845570 7.658704e-04 0.0031355618 20 58248115 58248273 159 - 1.053 1.334 1.000
ENSG00000124224 E033 13.1172448 0.0017052741 1.152675e-04 0.0005921759 20 58250887 58250952 66 - 0.953 1.300 1.248
ENSG00000124224 E034 13.1182451 0.0015167634 2.046041e-03 0.0073681499 20 58251757 58251864 108 - 1.011 1.281 0.966
ENSG00000124224 E035 0.3686942 0.0291919852 7.194050e-01 0.8142496986 20 58263372 58263517 146 - 0.099 0.150 0.680
ENSG00000124224 E036 15.7025955 0.0116780474 1.303648e-02 0.0359970550 20 58271363 58271505 143 - 1.108 1.334 0.796
ENSG00000124224 E037 15.9939888 0.0088536744 1.148669e-01 0.2126431512 20 58272769 58272875 107 - 1.166 1.299 0.472
ENSG00000124224 E038 17.6017782 0.0263290935 9.044878e-01 0.9431762991 20 58286314 58286446 133 - 1.267 1.270 0.008
ENSG00000124224 E039 12.2228604 0.0495933143 5.446706e-01 0.6751023878 20 58309226 58309270 45 - 1.158 1.065 -0.336
ENSG00000124224 E040 7.8059088 0.0504345368 3.892542e-01 0.5333650109 20 58309419 58309494 76 - 1.001 0.854 -0.555