ENSG00000124198

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000371917 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding protein_coding 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 2.3624489 0.3905269 5.36602392 0.19890301 0.358744633 3.74656864 0.33041667 0.1761667 0.557666667 0.38150000 0.23121299 0.03070131 FALSE TRUE
ENST00000679436 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding protein_coding 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 1.7960111 0.6781986 2.50212436 0.06452272 0.347513247 1.86801099 0.27167917 0.2881000 0.261466667 -0.02663333 0.92108137 0.03070131 FALSE TRUE
ENST00000679542 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding retained_intron 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 0.2597929 0.0000000 0.49772510 0.00000000 0.247672725 5.66597569 0.02822917 0.0000000 0.050633333 0.05063333 0.03070131 0.03070131 TRUE TRUE
ENST00000680130 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding retained_intron 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 0.1569179 0.5273216 0.08275009 0.28071506 0.045051394 -2.53436533 0.04974167 0.2035000 0.008566667 -0.19493333 0.22175093 0.03070131 FALSE FALSE
ENST00000681119 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding retained_intron 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 0.1900947 0.2626297 0.07232935 0.13246969 0.040340044 -1.72746385 0.07105833 0.1192000 0.007500000 -0.11170000 0.30315693 0.03070131 FALSE TRUE
ENST00000681399 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding nonsense_mediated_decay 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 0.2347870 0.1316823 0.00000000 0.09231368 0.000000000 -3.82458796 0.03936250 0.0583000 0.000000000 -0.05830000 0.03786035 0.03070131 FALSE TRUE
ENST00000681656 ENSG00000124198 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFGEF2 protein_coding nonsense_mediated_decay 6.241973 2.420934 9.616172 0.2142216 0.2304269 1.985451 0.4486521 0.1841310 0.18063090 0.18413105 0.006269964 -0.02624888 0.07150417 0.0648000 0.018800000 -0.04600000 1.00000000 0.03070131 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124198 E001 10.9132474 0.0135526050 9.711651e-04 3.860108e-03 20 48921711 48921828 118 + 1.023 0.523 -2.033
ENSG00000124198 E002 11.3904940 0.0104548839 7.875524e-03 2.346715e-02 20 48921829 48921889 61 + 1.029 0.689 -1.318
ENSG00000124198 E003 17.9854028 0.0161499433 1.326428e-01 2.377750e-01 20 48921890 48922010 121 + 1.185 1.074 -0.397
ENSG00000124198 E004 9.1405307 0.0096465486 1.036457e-01 1.960492e-01 20 48941199 48941229 31 + 0.929 0.752 -0.689
ENSG00000124198 E005 18.9886396 0.0009681119 2.325576e-02 5.827845e-02 20 48941864 48941987 124 + 1.215 1.045 -0.612
ENSG00000124198 E006 27.4607780 0.0006791196 3.650850e-03 1.215711e-02 20 48951323 48951469 147 + 1.369 1.175 -0.680
ENSG00000124198 E007 12.9189217 0.0013826573 3.064167e-02 7.321838e-02 20 48952705 48952724 20 + 1.066 0.858 -0.778
ENSG00000124198 E008 30.8778532 0.0007244067 2.097241e-01 3.382249e-01 20 48952725 48952884 160 + 1.393 1.371 -0.077
ENSG00000124198 E009 42.0112496 0.0004910688 8.793284e-02 1.719813e-01 20 48953556 48953790 235 + 1.525 1.484 -0.139
ENSG00000124198 E010 24.2153435 0.0007471939 1.270416e-01 2.300232e-01 20 48963830 48963898 69 + 1.300 1.238 -0.219
ENSG00000124198 E011 11.3397531 0.0015805896 1.869206e-01 3.098633e-01 20 48965872 48965874 3 + 1.000 0.902 -0.367
ENSG00000124198 E012 16.4940721 0.0011248364 4.191904e-01 5.624406e-01 20 48965875 48965877 3 + 1.136 1.128 -0.032
ENSG00000124198 E013 39.1968233 0.0005596754 2.163955e-02 5.492721e-02 20 48965878 48966023 146 + 1.505 1.412 -0.319
ENSG00000124198 E014 33.6676894 0.0007262327 1.660734e-02 4.407507e-02 20 48969147 48969277 131 + 1.443 1.326 -0.406
ENSG00000124198 E015 45.0407926 0.0008019428 1.267681e-04 6.445822e-04 20 48971120 48971354 235 + 1.580 1.371 -0.716
ENSG00000124198 E016 32.8384041 0.0022768585 2.832217e-02 6.864742e-02 20 48972326 48972425 100 + 1.432 1.326 -0.369
ENSG00000124198 E017 39.3537961 0.0031191730 7.333811e-03 2.208607e-02 20 48973145 48973284 140 + 1.510 1.371 -0.479
ENSG00000124198 E018 34.5594532 0.0022600287 4.564657e-03 1.472284e-02 20 48974766 48974874 109 + 1.458 1.293 -0.572
ENSG00000124198 E019 46.4713202 0.0005506525 4.252129e-06 3.072871e-05 20 48976016 48976199 184 + 1.598 1.326 -0.936
ENSG00000124198 E020 33.1850660 0.0006398315 2.238771e-02 5.648117e-02 20 48984729 48984840 112 + 1.435 1.326 -0.376
ENSG00000124198 E021 49.7487719 0.0038378430 9.551115e-04 3.805401e-03 20 48985408 48985613 206 + 1.613 1.438 -0.596
ENSG00000124198 E022 32.1630631 0.0090697320 8.733223e-04 3.520850e-03 20 48988304 48988388 85 + 1.441 1.176 -0.927
ENSG00000124198 E023 44.6440056 0.0005325773 3.031375e-02 7.258405e-02 20 48988491 48988662 172 + 1.555 1.484 -0.244
ENSG00000124198 E024 37.1957019 0.0005593427 3.094434e-02 7.380570e-02 20 48989285 48989436 152 + 1.484 1.399 -0.293
ENSG00000124198 E025 38.4685355 0.0045751272 5.714629e-04 2.427180e-03 20 48989556 48989684 129 + 1.514 1.292 -0.768
ENSG00000124198 E026 50.4895425 0.0035286055 3.540691e-02 8.243692e-02 20 48991040 48991198 159 + 1.607 1.536 -0.241
ENSG00000124198 E027 53.4203312 0.0014907059 5.053815e-01 6.411682e-01 20 48994451 48994598 148 + 1.610 1.648 0.132
ENSG00000124198 E028 43.8794198 0.0206093362 4.993055e-01 6.356216e-01 20 48995783 48995882 100 + 1.530 1.543 0.044
ENSG00000124198 E029 0.4418608 0.0274586439 7.531507e-01 8.391784e-01 20 48998100 48998192 93 + 0.149 0.000 -19.554
ENSG00000124198 E030 27.3538490 0.0009339907 8.266261e-01 8.909138e-01 20 48998193 48998233 41 + 1.315 1.412 0.339
ENSG00000124198 E031 44.4486108 0.0237081776 9.797630e-01 9.914068e-01 20 48998336 48998505 170 + 1.524 1.605 0.279
ENSG00000124198 E032 35.6785992 0.0006934561 6.770797e-01 7.818859e-01 20 49005070 49005221 152 + 1.426 1.536 0.380
ENSG00000124198 E033 38.0727092 0.0043807838 3.499401e-01 4.941311e-01 20 49010232 49010404 173 + 1.443 1.592 0.511
ENSG00000124198 E034 51.1336882 0.0009043585 2.704993e-01 4.091603e-01 20 49011924 49012084 161 + 1.570 1.718 0.503
ENSG00000124198 E035 0.4482035 0.0259994884 7.532803e-01 8.392865e-01 20 49013048 49013563 516 + 0.149 0.000 -19.555
ENSG00000124198 E036 42.9381322 0.0027938079 1.153347e-01 2.133396e-01 20 49013564 49013694 131 + 1.490 1.677 0.640
ENSG00000124198 E037 2.7892262 0.0418724060 4.924587e-01 6.296376e-01 20 49013695 49013830 136 + 0.503 0.408 -0.489
ENSG00000124198 E038 41.2450211 0.0006231700 7.422223e-01 8.311716e-01 20 49013831 49013960 130 + 1.497 1.556 0.199
ENSG00000124198 E039 42.6929425 0.0005494079 2.998580e-02 7.193153e-02 20 49016280 49016415 136 + 1.537 1.462 -0.259
ENSG00000124198 E040 41.9655479 0.0023491238 2.293740e-03 8.130642e-03 20 49017249 49017387 139 + 1.544 1.385 -0.546
ENSG00000124198 E041 28.0969203 0.0006816956 4.157302e-01 5.590928e-01 20 49017496 49017550 55 + 1.345 1.357 0.040
ENSG00000124198 E042 46.3635779 0.0004884592 9.943979e-01 1.000000e+00 20 49018884 49018998 115 + 1.544 1.625 0.276
ENSG00000124198 E043 0.8836079 0.0134373661 2.786086e-01 4.182683e-01 20 49018999 49019181 183 + 0.260 0.000 -20.554
ENSG00000124198 E044 1.6316200 0.0083330914 4.297265e-01 5.724057e-01 20 49022154 49023050 897 + 0.374 0.249 -0.815
ENSG00000124198 E045 51.6052486 0.0005424643 6.462053e-01 7.581790e-01 20 49023051 49023181 131 + 1.595 1.648 0.181
ENSG00000124198 E046 23.2132996 0.0008839723 8.801422e-01 9.271179e-01 20 49025313 49025316 4 + 1.260 1.326 0.231
ENSG00000124198 E047 56.9514021 0.0004028543 9.403839e-01 9.665729e-01 20 49025317 49025481 165 + 1.634 1.711 0.263
ENSG00000124198 E048 53.2336065 0.0027687553 7.759624e-02 1.556007e-01 20 49028530 49028668 139 + 1.581 1.772 0.647
ENSG00000124198 E049 0.4449813 0.0213110716 4.863192e-01 6.240846e-01 20 49031315 49032048 734 + 0.105 0.249 1.506
ENSG00000124198 E050 48.6114828 0.0005157833 4.512415e-02 1.004245e-01 20 49032049 49032166 118 + 1.542 1.743 0.682
ENSG00000124198 E051 5.6789234 0.0091467812 9.041402e-01 9.429944e-01 20 49032167 49033022 856 + 0.718 0.808 0.358
ENSG00000124198 E052 577.2048448 0.0109544765 8.313913e-09 1.040321e-07 20 49033023 49036693 3671 + 2.572 2.879 1.020