ENSG00000124191

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341197 ENSG00000124191 HEK293_OSMI2_2hA HEK293_TMG_2hB TOX2 protein_coding protein_coding 2.940965 5.71371 1.447641 0.66037 0.06993576 -1.973315 1.4261299 2.1526133 1.081911 0.7114118 0.06684356 -0.985921 0.5499333 0.3647000 0.7468 0.38210000 0.03346758 0.03346758 FALSE TRUE
ENST00000413823 ENSG00000124191 HEK293_OSMI2_2hA HEK293_TMG_2hB TOX2 protein_coding protein_coding 2.940965 5.71371 1.447641 0.66037 0.06993576 -1.973315 0.4637688 1.0395906 0.000000 0.6171051 0.00000000 -6.713683 0.1003250 0.1685333 0.0000 -0.16853333 0.31556723 0.03346758 FALSE TRUE
ENST00000423191 ENSG00000124191 HEK293_OSMI2_2hA HEK293_TMG_2hB TOX2 protein_coding protein_coding 2.940965 5.71371 1.447641 0.66037 0.06993576 -1.973315 0.3206590 0.6750369 0.000000 0.6750369 0.00000000 -6.098110 0.1158833 0.1382333 0.0000 -0.13823333 1.00000000 0.03346758 FALSE TRUE
MSTRG.20724.2 ENSG00000124191 HEK293_OSMI2_2hA HEK293_TMG_2hB TOX2 protein_coding   2.940965 5.71371 1.447641 0.66037 0.06993576 -1.973315 0.6735600 1.7531313 0.324852 0.3511854 0.03154306 -2.396545 0.2140833 0.3107333 0.2248 -0.08593333 0.64835943 0.03346758 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124191 E001 2.3800806 0.0314971307 0.0029657394 0.010160963 20 43914852 43914910 59 + 0.816 0.308 -2.427
ENSG00000124191 E002 5.5907835 0.0031016251 0.0236681440 0.059128069 20 43914911 43914990 80 + 0.955 0.647 -1.221
ENSG00000124191 E003 0.1482932 0.0421796279 1.0000000000   20 43916150 43916166 17 + 0.000 0.069 8.620
ENSG00000124191 E004 0.1482932 0.0421796279 1.0000000000   20 43916167 43916208 42 + 0.000 0.069 8.620
ENSG00000124191 E005 0.0000000       20 43945705 43946038 334 +      
ENSG00000124191 E006 0.0000000       20 43955148 43955272 125 +      
ENSG00000124191 E007 10.9216219 0.0023068212 0.1080448461 0.202637033 20 43973367 43973432 66 + 1.104 0.924 -0.662
ENSG00000124191 E008 33.2375644 0.0035766189 0.8172863105 0.884461313 20 44006547 44006792 246 + 1.409 1.427 0.060
ENSG00000124191 E009 0.0000000       20 44039004 44039229 226 +      
ENSG00000124191 E010 34.5636412 0.0007144416 0.2110224022 0.339740132 20 44051306 44051545 240 + 1.365 1.455 0.310
ENSG00000124191 E011 22.0348873 0.0009617661 0.9550643675 0.975785655 20 44054299 44054350 52 + 1.261 1.256 -0.021
ENSG00000124191 E012 41.9120500 0.0006282987 0.2843053883 0.424538720 20 44054351 44054526 176 + 1.572 1.507 -0.225
ENSG00000124191 E013 22.1116938 0.0012449929 0.0631510478 0.131958754 20 44064777 44064857 81 + 1.377 1.226 -0.529
ENSG00000124191 E014 31.4237994 0.0007375125 0.1007281356 0.191573568 20 44065712 44066107 396 + 1.494 1.381 -0.391
ENSG00000124191 E015 22.8464960 0.0008766749 0.4274154657 0.570265564 20 44066730 44066857 128 + 1.215 1.284 0.241
ENSG00000124191 E016 108.7066876 0.0003822399 0.0004894288 0.002119866 20 44068650 44069616 967 + 1.818 1.949 0.441