ENSG00000124160

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000290231 ENSG00000124160 HEK293_OSMI2_2hA HEK293_TMG_2hB NCOA5 protein_coding protein_coding 44.20515 51.56938 47.74255 3.42684 0.3440525 -0.1112166 34.006771 43.346331 32.475940 2.7657943 1.5934096 -0.4164272 0.76438333 0.8409333 0.6798667 -0.16106667 9.369248e-05 1.920747e-30 FALSE TRUE
ENST00000372291 ENSG00000124160 HEK293_OSMI2_2hA HEK293_TMG_2hB NCOA5 protein_coding protein_coding 44.20515 51.56938 47.74255 3.42684 0.3440525 -0.1112166 1.035574 0.000000 5.043322 0.0000000 0.6445252 8.9810883 0.02225833 0.0000000 0.1058000 0.10580000 1.920747e-30 1.920747e-30 TRUE FALSE
MSTRG.20792.2 ENSG00000124160 HEK293_OSMI2_2hA HEK293_TMG_2hB NCOA5 protein_coding   44.20515 51.56938 47.74255 3.42684 0.3440525 -0.1112166 8.599806 8.088358 10.223292 0.8440708 0.6963449 0.3375689 0.19853333 0.1565000 0.2143333 0.05783333 1.039407e-01 1.920747e-30 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000124160 E001 427.60035 0.0048402592 3.632158e-08 4.023044e-07 20 46060991 46061290 300 - 2.472 2.702 0.767
ENSG00000124160 E002 1955.50319 0.0018759244 2.658668e-13 7.120111e-12 20 46061291 46062483 1193 - 3.192 3.333 0.469
ENSG00000124160 E003 630.56904 0.0004610984 7.238690e-02 1.473157e-01 20 46062484 46062889 406 - 2.761 2.815 0.180
ENSG00000124160 E004 53.48688 0.0046942429 2.746152e-01 4.137936e-01 20 46063337 46063359 23 - 1.676 1.767 0.308
ENSG00000124160 E005 609.29410 0.0009175524 6.814370e-02 1.402869e-01 20 46063360 46063680 321 - 2.785 2.773 -0.039
ENSG00000124160 E006 487.99675 0.0031662673 4.670863e-02 1.033112e-01 20 46065029 46065228 200 - 2.703 2.664 -0.127
ENSG00000124160 E007 422.20390 0.0019139621 1.832700e-03 6.703857e-03 20 46067055 46067181 127 - 2.652 2.593 -0.199
ENSG00000124160 E008 429.53567 0.0049003045 3.416041e-04 1.546599e-03 20 46068502 46068638 137 - 2.687 2.579 -0.359
ENSG00000124160 E009 583.66217 0.0022171898 9.421768e-14 2.689761e-12 20 46070210 46070536 327 - 2.850 2.683 -0.556
ENSG00000124160 E010 193.50253 0.0046396258 1.073416e-07 1.084215e-06 20 46079387 46079453 67 - 2.387 2.188 -0.663
ENSG00000124160 E011 146.71576 0.0045387990 6.803317e-06 4.698972e-05 20 46089817 46089962 146 - 2.256 2.081 -0.586