ENSG00000123836

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367079 ENSG00000123836 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB2 protein_coding protein_coding 5.088802 4.379795 5.072487 1.1654 0.1880369 0.2113813 0.5252318 0.1636566 0.76957374 0.1636566 0.16138342 2.1664484 0.10477917 0.06076667 0.1525000 0.09173333 2.459626e-01 3.042286e-16 FALSE TRUE
ENST00000367080 ENSG00000123836 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB2 protein_coding protein_coding 5.088802 4.379795 5.072487 1.1654 0.1880369 0.2113813 2.6141511 1.4941159 3.06954711 0.2182358 0.02192647 1.0338025 0.51648333 0.36333333 0.6070667 0.24373333 6.306751e-03 3.042286e-16 FALSE TRUE
ENST00000464777 ENSG00000123836 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB2 protein_coding nonsense_mediated_decay 5.088802 4.379795 5.072487 1.1654 0.1880369 0.2113813 0.1005254 0.0000000 0.29280695 0.0000000 0.23456129 4.9203264 0.02095417 0.00000000 0.0547000 0.05470000 3.066914e-01 3.042286e-16   FALSE
ENST00000473310 ENSG00000123836 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB2 protein_coding processed_transcript 5.088802 4.379795 5.072487 1.1654 0.1880369 0.2113813 0.5118781 0.7728326 0.00000000 0.1772243 0.00000000 -6.2906318 0.09626667 0.18043333 0.0000000 -0.18043333 3.042286e-16 3.042286e-16   FALSE
ENST00000483688 ENSG00000123836 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB2 protein_coding processed_transcript 5.088802 4.379795 5.072487 1.1654 0.1880369 0.2113813 0.6430370 1.4838431 0.01563502 0.6559580 0.01563502 -5.8647687 0.12338333 0.30666667 0.0031000 -0.30356667 4.572995e-10 3.042286e-16   FALSE
MSTRG.2956.5 ENSG00000123836 HEK293_OSMI2_2hA HEK293_TMG_2hB PFKFB2 protein_coding   5.088802 4.379795 5.072487 1.1654 0.1880369 0.2113813 0.5332570 0.4015628 0.79894983 0.2668496 0.18246861 0.9749377 0.10620417 0.07163333 0.1573000 0.08566667 4.748105e-01 3.042286e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000123836 E001 0.0000000       1 207034366 207034415 50 +      
ENSG00000123836 E002 0.0000000       1 207034416 207034472 57 +      
ENSG00000123836 E003 0.0000000       1 207034946 207035080 135 +      
ENSG00000123836 E004 0.0000000       1 207042169 207042212 44 +      
ENSG00000123836 E005 0.1515154 0.0459910290 7.374577e-01   1 207049157 207049299 143 + 0.098 0.000 -8.089
ENSG00000123836 E006 0.1472490 0.0452507863 7.378329e-01   1 207049464 207049617 154 + 0.098 0.000 -10.018
ENSG00000123836 E007 0.0000000       1 207049618 207050042 425 +      
ENSG00000123836 E008 2.7248414 0.0058014079 8.569645e-01 9.116090e-01 1 207053055 207053207 153 + 0.578 0.594 0.070
ENSG00000123836 E009 4.4762618 0.0438240483 3.933878e-01 5.372746e-01 1 207053275 207053282 8 + 0.782 0.677 -0.431
ENSG00000123836 E010 6.9389471 0.0740174184 3.202614e-01 4.633616e-01 1 207053283 207053310 28 + 0.958 0.806 -0.582
ENSG00000123836 E011 12.5906711 0.0222871330 1.160881e-01 2.143763e-01 1 207053311 207053366 56 + 1.188 1.041 -0.527
ENSG00000123836 E012 21.8420878 0.0013202616 3.068164e-03 1.046739e-02 1 207054701 207054802 102 + 1.420 1.249 -0.598
ENSG00000123836 E013 0.2998086 0.0277486093 7.898778e-01   1 207054803 207054955 153 + 0.098 0.152 0.722
ENSG00000123836 E014 1.3380404 0.3187242508 3.855292e-02 8.837159e-02 1 207060846 207060930 85 + 0.516 0.000 -12.627
ENSG00000123836 E015 25.8138902 0.0007727225 2.117215e-06 1.637878e-05 1 207061953 207062078 126 + 1.524 1.238 -0.990
ENSG00000123836 E016 1.3159143 0.0545210573 5.909571e-01 7.137721e-01 1 207062547 207062619 73 + 0.304 0.428 0.729
ENSG00000123836 E017 26.7167128 0.0007858716 3.768826e-05 2.186473e-04 1 207062620 207062716 97 + 1.527 1.297 -0.796
ENSG00000123836 E018 12.5496939 0.0015975764 3.311787e-05 1.949138e-04 1 207063143 207063146 4 + 1.254 0.886 -1.343
ENSG00000123836 E019 20.5704610 0.0009752783 1.885335e-03 6.868939e-03 1 207063147 207063209 63 + 1.403 1.217 -0.652
ENSG00000123836 E020 21.7649575 0.0009686008 9.126175e-02 1.771023e-01 1 207063347 207063421 75 + 1.385 1.315 -0.245
ENSG00000123836 E021 20.3714148 0.0009746199 7.598019e-01 8.440675e-01 1 207063773 207063829 57 + 1.295 1.372 0.269
ENSG00000123836 E022 30.3919223 0.0041094382 1.491807e-01 2.605498e-01 1 207065036 207065160 125 + 1.517 1.473 -0.151
ENSG00000123836 E023 45.7229959 0.0043669262 3.816002e-03 1.262561e-02 1 207067499 207067706 208 + 1.717 1.598 -0.403
ENSG00000123836 E024 34.5914482 0.0006629770 1.243728e-03 4.789232e-03 1 207068163 207068309 147 + 1.602 1.463 -0.477
ENSG00000123836 E025 24.0689048 0.0055796121 1.389982e-02 3.799990e-02 1 207069424 207069528 105 + 1.452 1.306 -0.508
ENSG00000123836 E026 21.2793036 0.0010974809 8.014943e-02 1.597734e-01 1 207070280 207070409 130 + 1.381 1.306 -0.261
ENSG00000123836 E027 0.5901540 0.0199193062 5.225583e-01 6.563445e-01 1 207070645 207070764 120 + 0.245 0.152 -0.862
ENSG00000123836 E028 16.6111303 0.0011563193 4.072178e-01 5.508086e-01 1 207071188 207071250 63 + 1.260 1.248 -0.043
ENSG00000123836 E029 21.1839288 0.0013082818 6.881470e-02 1.414070e-01 1 207071509 207071573 65 + 1.385 1.306 -0.278
ENSG00000123836 E030 195.0916845 0.0109671723 6.725822e-02 1.387988e-01 1 207072204 207075259 3056 + 2.310 2.259 -0.170
ENSG00000123836 E031 215.6569500 0.0097636050 8.289679e-12 1.748641e-10 1 207075260 207077817 2558 + 2.153 2.502 1.165
ENSG00000123836 E032 83.3655694 0.0007732656 2.998506e-09 4.067435e-08 1 207078954 207081024 2071 + 1.791 2.074 0.952