ENSG00000123485

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373395 ENSG00000123485 HEK293_OSMI2_2hA HEK293_TMG_2hB HJURP protein_coding retained_intron 22.97 15.2007 30.35028 1.34615 1.287108 0.9970981 2.0508316 1.0367600 1.767308 0.33801748 0.3716879 0.7637634 0.09734583 0.07300000 0.05913333 -0.01386667 9.634879e-01 8.526733e-06   FALSE
ENST00000411486 ENSG00000123485 HEK293_OSMI2_2hA HEK293_TMG_2hB HJURP protein_coding protein_coding 22.97 15.2007 30.35028 1.34615 1.287108 0.9970981 12.4504924 11.4435442 14.737752 1.27957002 0.1549973 0.3647010 0.56082500 0.75086667 0.48770000 -0.26316667 8.526733e-06 8.526733e-06 FALSE TRUE
ENST00000414924 ENSG00000123485 HEK293_OSMI2_2hA HEK293_TMG_2hB HJURP protein_coding protein_coding 22.97 15.2007 30.35028 1.34615 1.287108 0.9970981 4.5973046 0.3223653 7.859806 0.32236527 0.7684511 4.5654865 0.17801667 0.02186667 0.25896667 0.23710000 2.775313e-02 8.526733e-06 FALSE FALSE
ENST00000453122 ENSG00000123485 HEK293_OSMI2_2hA HEK293_TMG_2hB HJURP protein_coding nonsense_mediated_decay 22.97 15.2007 30.35028 1.34615 1.287108 0.9970981 0.7839428 0.2184334 2.235225 0.05534034 0.9078825 3.2970143 0.03019583 0.01510000 0.07206667 0.05696667 2.946607e-02 8.526733e-06 TRUE TRUE
MSTRG.20099.10 ENSG00000123485 HEK293_OSMI2_2hA HEK293_TMG_2hB HJURP protein_coding   22.97 15.2007 30.35028 1.34615 1.287108 0.9970981 2.4032070 1.3233890 3.472800 0.23025741 0.5269808 1.3851497 0.09935833 0.08616667 0.11346667 0.02730000 5.186840e-01 8.526733e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000123485 E001 1.2480582 0.0104776312 1.576012e-02 4.219426e-02 2 233833416 233833732 317 - 0.100 0.537 3.235
ENSG00000123485 E002 0.2998086 0.0282634872 7.998899e-01   2 233836701 233836701 1 - 0.100 0.148 0.646
ENSG00000123485 E003 27.1678079 0.0041311248 1.080090e-06 8.901161e-06 2 233836702 233837276 575 - 1.233 1.625 1.352
ENSG00000123485 E004 213.6124789 0.0092745898 2.688097e-06 2.033754e-05 2 233837277 233837652 376 - 2.187 2.467 0.935
ENSG00000123485 E005 259.0127871 0.0032958118 2.342702e-08 2.694215e-07 2 233840609 233840814 206 - 2.303 2.528 0.751
ENSG00000123485 E006 701.3290579 0.0045794953 4.412024e-01 5.828438e-01 2 233840815 233841850 1036 - 2.817 2.878 0.200
ENSG00000123485 E007 104.5120555 0.0003127387 3.633244e-04 1.632065e-03 2 233841851 233841892 42 - 2.055 1.971 -0.283
ENSG00000123485 E008 216.7430965 0.0002209987 9.578668e-04 3.814533e-03 2 233841893 233842115 223 - 2.353 2.314 -0.128
ENSG00000123485 E009 135.1315345 0.0002332604 4.000183e-03 1.314250e-02 2 233842116 233842205 90 - 2.153 2.106 -0.156
ENSG00000123485 E010 84.4195959 0.0094891462 7.796016e-01 8.582921e-01 2 233844205 233844224 20 - 1.922 1.934 0.040
ENSG00000123485 E011 100.8973826 0.0023077465 3.706931e-01 5.151002e-01 2 233844225 233844283 59 - 2.006 2.005 -0.002
ENSG00000123485 E012 99.3403801 0.0028919195 1.979144e-02 5.104529e-02 2 233845728 233845820 93 - 2.026 1.961 -0.220
ENSG00000123485 E013 12.0911853 0.0767592757 5.895220e-01 7.125856e-01 2 233845821 233846135 315 - 1.130 1.076 -0.193
ENSG00000123485 E014 78.1232115 0.0003755974 2.773807e-05 1.662889e-04 2 233847397 233847461 65 - 1.949 1.818 -0.440
ENSG00000123485 E015 98.0222114 0.0021568542 4.079013e-04 1.804747e-03 2 233849763 233849859 97 - 2.038 1.927 -0.373
ENSG00000123485 E016 22.4182029 0.0031151505 1.699882e-01 2.881845e-01 2 233852029 233852214 186 - 1.396 1.330 -0.232
ENSG00000123485 E017 80.6186397 0.0057278283 2.523569e-03 8.833098e-03 2 233852565 233852620 56 - 1.960 1.831 -0.434
ENSG00000123485 E018 77.8429449 0.0043722908 5.610391e-06 3.948260e-05 2 233853844 233853910 67 - 1.970 1.770 -0.674
ENSG00000123485 E019 74.4830638 0.0083132933 3.237615e-03 1.096623e-02 2 233854384 233854566 183 - 1.932 1.787 -0.487