ENSG00000123213

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380985 ENSG00000123213 HEK293_OSMI2_2hA HEK293_TMG_2hB NLN protein_coding protein_coding 12.48322 3.314966 22.06123 0.138296 0.4322859 2.730755 4.4947194 1.6644581 7.9583465 0.34418274 0.4508582 2.2505861 0.42863750 0.51053333 0.360933333 -0.14960000 5.472737e-01 6.333485e-05 FALSE TRUE
ENST00000506539 ENSG00000123213 HEK293_OSMI2_2hA HEK293_TMG_2hB NLN protein_coding retained_intron 12.48322 3.314966 22.06123 0.138296 0.4322859 2.730755 2.9772260 0.0905523 6.3044631 0.07205466 0.6831612 5.9726421 0.14543750 0.02566667 0.284833333 0.25916667 6.333485e-05 6.333485e-05 FALSE TRUE
ENST00000509935 ENSG00000123213 HEK293_OSMI2_2hA HEK293_TMG_2hB NLN protein_coding protein_coding 12.48322 3.314966 22.06123 0.138296 0.4322859 2.730755 3.3890028 0.5295171 5.7223812 0.27125586 0.2490989 3.4093940 0.22779583 0.15610000 0.259166667 0.10306667 6.660138e-01 6.333485e-05 FALSE TRUE
ENST00000514991 ENSG00000123213 HEK293_OSMI2_2hA HEK293_TMG_2hB NLN protein_coding nonsense_mediated_decay 12.48322 3.314966 22.06123 0.138296 0.4322859 2.730755 0.7719265 0.0000000 1.3471964 0.00000000 0.8043765 7.0844857 0.04130000 0.00000000 0.062433333 0.06243333 4.646592e-01 6.333485e-05 FALSE FALSE
MSTRG.26287.3 ENSG00000123213 HEK293_OSMI2_2hA HEK293_TMG_2hB NLN protein_coding   12.48322 3.314966 22.06123 0.138296 0.4322859 2.730755 0.3327248 0.2683505 0.3981502 0.09477396 0.0379513 0.5521974 0.04229583 0.07893333 0.018033333 -0.06090000 1.948799e-03 6.333485e-05 FALSE TRUE
MSTRG.26287.7 ENSG00000123213 HEK293_OSMI2_2hA HEK293_TMG_2hB NLN protein_coding   12.48322 3.314966 22.06123 0.138296 0.4322859 2.730755 0.3007648 0.7620877 0.1456050 0.41223102 0.1456050 -2.3108768 0.08475417 0.22876667 0.006366667 -0.22240000 2.235805e-01 6.333485e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000123213 E001 1.7819775 0.1534808295 6.758721e-02 1.393457e-01 5 65722205 65722213 9 + 0.470 0.000 -11.944
ENSG00000123213 E002 7.9187592 0.0024733193 3.456171e-04 1.562415e-03 5 65722214 65722238 25 + 0.967 0.363 -2.662
ENSG00000123213 E003 9.9996149 0.0054035778 2.584220e-05 1.561536e-04 5 65722239 65722242 4 + 1.062 0.363 -3.012
ENSG00000123213 E004 10.1511304 0.0067825230 2.407053e-05 1.466078e-04 5 65722243 65722246 4 + 1.068 0.363 -3.034
ENSG00000123213 E005 18.0323257 0.0031426858 3.489318e-08 3.878438e-07 5 65722247 65722257 11 + 1.300 0.558 -2.860
ENSG00000123213 E006 63.9815128 0.0128245026 6.281255e-08 6.636356e-07 5 65722258 65722414 157 + 1.810 1.354 -1.555
ENSG00000123213 E007 3.6254633 0.1686307900 8.111904e-01 8.802056e-01 5 65740895 65741000 106 + 0.611 0.565 -0.206
ENSG00000123213 E008 0.7322062 0.2225206009 3.833288e-01 5.275199e-01 5 65752607 65752663 57 + 0.257 0.000 -12.090
ENSG00000123213 E009 140.5233552 0.0057222568 2.401487e-07 2.267290e-06 5 65758567 65758826 260 + 2.125 1.864 -0.876
ENSG00000123213 E010 118.6333876 0.0013659704 5.123550e-04 2.205950e-03 5 65762960 65763108 149 + 2.032 1.903 -0.433
ENSG00000123213 E011 0.5954526 0.0172671820 3.947521e-01 5.385814e-01 5 65777417 65777426 10 + 0.217 0.000 -12.936
ENSG00000123213 E012 109.4202428 0.0007922710 6.382699e-05 3.501834e-04 5 65777427 65777534 108 + 2.001 1.847 -0.519
ENSG00000123213 E013 0.0000000       5 65780028 65780178 151 +      
ENSG00000123213 E014 90.9218624 0.0003549455 1.895356e-02 4.923145e-02 5 65780179 65780281 103 + 1.909 1.826 -0.280
ENSG00000123213 E015 115.2234416 0.0007803491 2.862403e-03 9.860059e-03 5 65781261 65781415 155 + 2.015 1.913 -0.342
ENSG00000123213 E016 48.9098379 0.0004309015 5.565603e-02 1.191075e-01 5 65781416 65781421 6 + 1.646 1.552 -0.322
ENSG00000123213 E017 110.7749472 0.0003155660 3.564453e-03 1.191087e-02 5 65785775 65785910 136 + 1.997 1.900 -0.326
ENSG00000123213 E018 0.1451727 0.0429358563 1.000000e+00   5 65785911 65786060 150 + 0.065 0.000 -10.560
ENSG00000123213 E019 176.2617315 0.0002212709 5.349906e-03 1.688306e-02 5 65788118 65788362 245 + 2.189 2.124 -0.220
ENSG00000123213 E020 119.3725993 0.0003997675 6.374844e-02 1.329343e-01 5 65788363 65788484 122 + 2.018 1.969 -0.165
ENSG00000123213 E021 127.6950890 0.0013670140 8.512395e-01 9.077579e-01 5 65792454 65792601 148 + 2.034 2.051 0.057
ENSG00000123213 E022 85.3997855 0.0003729255 4.083420e-01 5.519347e-01 5 65792602 65792655 54 + 1.868 1.854 -0.046
ENSG00000123213 E023 0.6717251 0.0311550820 8.354948e-01 8.971264e-01 5 65792656 65793649 994 + 0.172 0.219 0.428
ENSG00000123213 E024 0.0000000       5 65809126 65809409 284 +      
ENSG00000123213 E025 142.7154291 0.0002189635 3.181829e-01 4.611432e-01 5 65809515 65809701 187 + 2.071 2.130 0.196
ENSG00000123213 E026 104.3620436 0.0004890530 1.127601e-02 3.182099e-02 5 65810037 65810165 129 + 1.919 2.044 0.419
ENSG00000123213 E027 115.4827875 0.0003416557 2.491109e-04 1.171457e-03 5 65812255 65812391 137 + 1.951 2.111 0.535
ENSG00000123213 E028 0.2966881 0.0274424043 1.000000e+00   5 65818721 65818983 263 + 0.122 0.000 -11.762
ENSG00000123213 E029 736.4805973 0.0014284179 2.219631e-25 2.513464e-23 5 65822781 65829283 6503 + 2.739 2.943 0.681
ENSG00000123213 E030 0.0000000       5 65860808 65860855 48 +      
ENSG00000123213 E031 0.0000000       5 65871445 65871725 281 +