ENSG00000123094

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000405154 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding protein_coding 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.96818735 0.00000000 0.5181412 0.00000000 0.33380182 5.722852 0.16704583 0.00000000 0.08316667 0.083166667 6.127188e-01 2.951545e-05 FALSE TRUE
ENST00000538081 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding processed_transcript 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.07100637 0.04870985 0.1434675 0.04870985 0.07173943 1.386259 0.05490417 0.04586667 0.02073333 -0.025133333 8.820918e-01 2.951545e-05   FALSE
ENST00000539545 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding nonsense_mediated_decay 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.13355298 0.30975016 0.0000000 0.17364643 0.00000000 -4.998873 0.11575417 0.31676667 0.00000000 -0.316766667 1.575552e-02 2.951545e-05 TRUE TRUE
ENST00000541490 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding protein_coding 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.68841029 0.28023847 1.9793361 0.15268606 0.38538497 2.776976 0.23947917 0.30403333 0.30520000 0.001166667 9.528162e-01 2.951545e-05 FALSE TRUE
ENST00000542004 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding protein_coding 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.52909137 0.00000000 1.1335246 0.00000000 0.57428701 6.837344 0.08207500 0.00000000 0.16336667 0.163366667 4.647337e-01 2.951545e-05 FALSE TRUE
ENST00000542865 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding protein_coding 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.11999630 0.00000000 0.6809951 0.00000000 0.34119140 6.110604 0.01959167 0.00000000 0.09853333 0.098533333 5.202001e-01 2.951545e-05 FALSE TRUE
ENST00000688511 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding protein_coding 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.25704059 0.22451055 0.0000000 0.22451055 0.00000000 -4.551581 0.15299167 0.33333333 0.00000000 -0.333333333 7.471929e-01 2.951545e-05 FALSE TRUE
ENST00000689635 ENSG00000123094 HEK293_OSMI2_2hA HEK293_TMG_2hB RASSF8 protein_coding protein_coding 3.54302 0.863209 6.602211 0.112253 0.3450453 2.920734 0.48272387 0.00000000 1.6424283 0.00000000 0.31286188 7.368444 0.08887083 0.00000000 0.24803333 0.248033333 2.951545e-05 2.951545e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000123094 E001 0.1515154 0.0425651227 6.778318e-01   12 25958232 25958342 111 + 0.042 0.000 -7.679
ENSG00000123094 E002 6.1937880 0.0286318949 5.128711e-03 1.627939e-02 12 25958682 25959028 347 + 0.724 0.000 -13.636
ENSG00000123094 E003 1.4790604 0.0091054869 2.792149e-01 4.189318e-01 12 25959029 25959057 29 + 0.306 0.000 -12.195
ENSG00000123094 E004 0.8772652 0.1037923974 6.261368e-01 7.421294e-01 12 25959058 25959064 7 + 0.208 0.000 -11.108
ENSG00000123094 E005 1.7639935 0.0786057332 8.042288e-01 8.754639e-01 12 25959065 25959080 16 + 0.327 0.307 -0.126
ENSG00000123094 E006 3.3988358 0.0045927564 2.644942e-01 4.024561e-01 12 25959081 25959148 68 + 0.512 0.308 -1.122
ENSG00000123094 E007 0.0000000       12 25973755 25973887 133 +      
ENSG00000123094 E008 0.0000000       12 25995004 25995036 33 +      
ENSG00000123094 E009 6.8342033 0.0037926887 3.165620e-01 4.593493e-01 12 25995037 25995130 94 + 0.732 0.613 -0.504
ENSG00000123094 E010 0.0000000       12 26011736 26011777 42 +      
ENSG00000123094 E011 0.0000000       12 26011778 26011821 44 +      
ENSG00000123094 E012 0.9148410 0.0145322804 1.413186e-01 2.498426e-01 12 26020145 26020504 360 + 0.149 0.487 2.337
ENSG00000123094 E013 0.0000000       12 26021440 26021518 79 +      
ENSG00000123094 E014 0.0000000       12 26052564 26052568 5 +      
ENSG00000123094 E015 0.0000000       12 26052569 26052602 34 +      
ENSG00000123094 E016 0.0000000       12 26052603 26052996 394 +      
ENSG00000123094 E017 4.2157467 0.0038490063 5.086774e-01 6.440481e-01 12 26055236 26055278 43 + 0.564 0.487 -0.363
ENSG00000123094 E018 22.0082939 0.0009099647 2.791094e-05 1.672035e-04 12 26055279 26055446 168 + 1.200 0.613 -2.257
ENSG00000123094 E019 59.5520702 0.0018534554 3.892718e-08 4.290323e-07 12 26064498 26065027 530 + 1.606 1.190 -1.443
ENSG00000123094 E020 57.2041435 0.0029671406 4.548199e-03 1.467575e-02 12 26065028 26065387 360 + 1.572 1.430 -0.488
ENSG00000123094 E021 27.0781223 0.0006995674 1.599362e-03 5.959530e-03 12 26067569 26067713 145 + 1.272 0.967 -1.097
ENSG00000123094 E022 28.8193123 0.0006713090 5.057889e-03 1.608909e-02 12 26068697 26068923 227 + 1.293 1.055 -0.848
ENSG00000123094 E023 183.4210866 0.0002847982 5.905301e-01 7.133740e-01 12 26068924 26071655 2732 + 2.044 2.148 0.350
ENSG00000123094 E024 81.3025852 0.0264128261 1.987648e-11 3.940086e-10 12 26071656 26072871 1216 + 1.600 2.168 1.915
ENSG00000123094 E025 0.1515154 0.0425651227 6.778318e-01   12 26079033 26079892 860 + 0.042 0.000 -8.925