ENSG00000122971

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000242592 ENSG00000122971 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADS protein_coding protein_coding 14.40241 26.48977 10.15532 1.37297 0.1149093 -1.382325 12.769379 24.890944 8.910892 1.1070155 0.03561411 -1.48094050 0.8573875 0.94036667 0.8776667 -0.0627 3.829865e-03 1.772398e-05 FALSE  
ENST00000539690 ENSG00000122971 HEK293_OSMI2_2hA HEK293_TMG_2hB ACADS protein_coding retained_intron 14.40241 26.48977 10.15532 1.37297 0.1149093 -1.382325 1.480784 1.170408 1.155754 0.1691756 0.05317757 -0.01802274 0.1322542 0.04373333 0.1137333 0.0700 1.772398e-05 1.772398e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122971 E001 1.10611 0.0131794991 1.100504e-02 3.117425e-02 12 120725774 120725825 52 + 0.582 0.138 -2.912
ENSG00000122971 E002 18.65653 0.0016303363 6.624470e-01 7.707483e-01 12 120725826 120725860 35 + 1.240 1.199 -0.143
ENSG00000122971 E003 92.21200 0.0032095307 3.630325e-02 8.415914e-02 12 120725861 120725931 71 + 1.955 1.855 -0.336
ENSG00000122971 E004 178.21694 0.0002794895 6.704117e-04 2.790270e-03 12 120727026 120727189 164 + 2.240 2.140 -0.334
ENSG00000122971 E005 179.94456 0.0002342489 1.939186e-02 5.018309e-02 12 120736986 120737135 150 + 2.221 2.152 -0.231
ENSG00000122971 E006 14.68494 0.0012409690 3.873463e-05 2.239907e-04 12 120737136 120737355 220 + 1.373 1.000 -1.327
ENSG00000122971 E007 121.38210 0.0002710521 3.495758e-01 4.937868e-01 12 120737356 120737467 112 + 2.028 1.993 -0.117
ENSG00000122971 E008 11.27056 0.0015117893 2.712069e-02 6.624120e-02 12 120737468 120737805 338 + 1.179 0.948 -0.842
ENSG00000122971 E009 160.01387 0.0005582263 5.557262e-01 6.845474e-01 12 120737837 120737988 152 + 2.107 2.126 0.064
ENSG00000122971 E010 3.13131 0.0049894114 4.331940e-01 5.756179e-01 12 120738049 120738188 140 + 0.642 0.512 -0.589
ENSG00000122971 E011 208.79039 0.0003273850 4.836551e-01 6.216932e-01 12 120738280 120738450 171 + 2.221 2.240 0.064
ENSG00000122971 E012 184.81292 0.0017875580 6.076539e-01 7.275831e-01 12 120738533 120738670 138 + 2.170 2.188 0.063
ENSG00000122971 E013 140.03337 0.0002422510 4.524804e-01 5.933238e-01 12 120738820 120738915 96 + 2.041 2.067 0.085
ENSG00000122971 E014 102.60098 0.0002899818 3.228309e-02 7.639856e-02 12 120739140 120739196 57 + 1.855 1.944 0.300
ENSG00000122971 E015 342.42358 0.0001704884 2.712676e-07 2.531817e-06 12 120739296 120740008 713 + 2.357 2.469 0.375