ENSG00000122756

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000351266 ENSG00000122756 HEK293_OSMI2_2hA HEK293_TMG_2hB CNTFR protein_coding protein_coding 2.796411 4.758622 2.094466 0.4673919 0.07585338 -1.180118 0.2234079 0.2637884 0.2294181 0.2040764 0.2294181 -0.1935294 0.0729000 0.05083333 0.1149667 0.06413333 8.962061e-01 4.229717e-08 FALSE TRUE
ENST00000378980 ENSG00000122756 HEK293_OSMI2_2hA HEK293_TMG_2hB CNTFR protein_coding protein_coding 2.796411 4.758622 2.094466 0.4673919 0.07585338 -1.180118 1.6427747 1.6329994 1.8650478 0.5266077 0.2846797 0.1905955 0.6162667 0.33330000 0.8850333 0.55173333 4.680195e-02 4.229717e-08 FALSE TRUE
ENST00000610543 ENSG00000122756 HEK293_OSMI2_2hA HEK293_TMG_2hB CNTFR protein_coding protein_coding 2.796411 4.758622 2.094466 0.4673919 0.07585338 -1.180118 0.8582505 2.8618339 0.0000000 0.5427375 0.0000000 -8.1658285 0.2785958 0.61593333 0.0000000 -0.61593333 4.229717e-08 4.229717e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122756 E001 0.0000000       9 34551432 34551432 1 -      
ENSG00000122756 E002 0.0000000       9 34551433 34551433 1 -      
ENSG00000122756 E003 60.9856965 0.0005065220 0.02095360 0.05349357 9 34551434 34552071 638 - 1.639 1.750 0.376
ENSG00000122756 E004 44.6319664 0.0005846399 0.05278743 0.11411949 9 34552161 34552329 169 - 1.500 1.611 0.382
ENSG00000122756 E005 50.5460809 0.0005212942 0.50902612 0.64438048 9 34552674 34552854 181 - 1.617 1.651 0.113
ENSG00000122756 E006 40.8372431 0.0006042654 0.07580534 0.15278734 9 34556255 34556418 164 - 1.628 1.528 -0.344
ENSG00000122756 E007 34.6474861 0.0006594137 0.06767864 0.13949599 9 34557526 34557692 167 - 1.566 1.453 -0.386
ENSG00000122756 E008 23.6778222 0.0067410736 0.36562005 0.50993788 9 34557867 34557984 118 - 1.377 1.303 -0.258
ENSG00000122756 E009 36.8487071 0.0006155934 0.88370322 0.92961294 9 34564599 34564832 234 - 1.514 1.503 -0.035
ENSG00000122756 E010 23.6243623 0.0114047474 0.64876738 0.76023402 9 34568897 34568981 85 - 1.294 1.337 0.151
ENSG00000122756 E011 16.1353419 0.0099987821 0.83598876 0.89744926 9 34581095 34581205 111 - 1.176 1.158 -0.065
ENSG00000122756 E012 7.0647317 0.0232533314 0.01383394 0.03784835 9 34589555 34589724 170 - 1.056 0.728 -1.254
ENSG00000122756 E013 0.4031496 0.0241830894 0.59360046 0.71597817 9 34590082 34590140 59 - 0.000 0.149 8.912