ENSG00000122729

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000309951 ENSG00000122729 HEK293_OSMI2_2hA HEK293_TMG_2hB ACO1 protein_coding protein_coding 17.70394 17.44427 17.26712 2.124187 0.3537648 -0.01471727 4.497779 5.548612 3.005645 1.157869 0.2859227 -0.8822581 0.2554417 0.3131 0.1747667 -0.1383333 0.028804368 0.004909399 FALSE TRUE
MSTRG.32514.4 ENSG00000122729 HEK293_OSMI2_2hA HEK293_TMG_2hB ACO1 protein_coding   17.70394 17.44427 17.26712 2.124187 0.3537648 -0.01471727 11.969529 10.353849 12.338630 1.249584 0.5008081 0.2527910 0.6729500 0.5941 0.7140333 0.1199333 0.004909399 0.004909399 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122729 E001 1.3704194 0.0124104914 4.612650e-03 1.485462e-02 9 32384603 32384619 17 + 0.596 0.110 -3.353
ENSG00000122729 E002 1.9563070 0.0074368280 2.228375e-03 7.930698e-03 9 32384620 32384628 9 + 0.703 0.197 -2.814
ENSG00000122729 E003 5.0522297 0.0446591860 2.535555e-01 3.900839e-01 9 32384629 32384636 8 + 0.861 0.681 -0.721
ENSG00000122729 E004 6.2971569 0.0378904124 9.650949e-02 1.851281e-01 9 32384637 32384642 6 + 0.976 0.730 -0.952
ENSG00000122729 E005 47.9406707 0.0106421871 7.962203e-04 3.245859e-03 9 32384643 32384735 93 + 1.806 1.574 -0.785
ENSG00000122729 E006 3.9364828 0.0039596768 9.402014e-02 1.814109e-01 9 32386371 32386438 68 + 0.814 0.589 -0.940
ENSG00000122729 E007 0.5483223 0.0235208572 7.334243e-01 8.246239e-01 9 32386439 32386442 4 + 0.136 0.198 0.649
ENSG00000122729 E008 82.9728401 0.0003545127 1.909625e-08 2.235510e-07 9 32405485 32405603 119 + 2.029 1.827 -0.679
ENSG00000122729 E009 112.1900026 0.0028851573 4.663826e-06 3.342993e-05 9 32407261 32407429 169 + 2.146 1.967 -0.601
ENSG00000122729 E010 107.7965242 0.0087566281 1.046661e-02 2.990075e-02 9 32408514 32408651 138 + 2.104 1.973 -0.441
ENSG00000122729 E011 85.2120926 0.0019277231 1.154650e-03 4.486984e-03 9 32418128 32418197 70 + 2.003 1.878 -0.420
ENSG00000122729 E012 85.3938317 0.0063622652 1.357943e-02 3.725950e-02 9 32418328 32418376 49 + 2.000 1.877 -0.413
ENSG00000122729 E013 118.3881949 0.0002583442 1.824145e-05 1.142032e-04 9 32418377 32418511 135 + 2.144 2.020 -0.414
ENSG00000122729 E014 134.9521635 0.0078942098 4.420374e-02 9.876712e-02 9 32419038 32419177 140 + 2.182 2.088 -0.317
ENSG00000122729 E015 139.2659325 0.0054841341 1.971723e-01 3.226934e-01 9 32420856 32421027 172 + 2.171 2.122 -0.162
ENSG00000122729 E016 93.6237786 0.0003484132 6.319355e-01 7.468762e-01 9 32423319 32423419 101 + 1.974 1.970 -0.012
ENSG00000122729 E017 140.1578847 0.0002630941 3.484505e-01 4.926850e-01 9 32424549 32424665 117 + 2.154 2.140 -0.046
ENSG00000122729 E018 72.5885701 0.0004020182 5.263567e-01 6.595963e-01 9 32425838 32425841 4 + 1.868 1.856 -0.042
ENSG00000122729 E019 195.4737797 0.0004956941 4.711297e-03 1.512756e-02 9 32425842 32425997 156 + 2.324 2.263 -0.206
ENSG00000122729 E020 175.8599499 0.0002633956 2.418122e-04 1.140986e-03 9 32427301 32427436 136 + 2.293 2.209 -0.280
ENSG00000122729 E021 138.9447897 0.0002790658 2.484662e-01 3.841197e-01 9 32429419 32429503 85 + 2.157 2.137 -0.067
ENSG00000122729 E022 180.1478093 0.0002218557 3.360399e-03 1.132268e-02 9 32430418 32430574 157 + 2.292 2.230 -0.209
ENSG00000122729 E023 193.2267685 0.0002592591 2.039536e-01 3.311342e-01 9 32431719 32431843 125 + 2.295 2.276 -0.063
ENSG00000122729 E024 176.0078857 0.0010765991 6.605711e-03 2.020101e-02 9 32433728 32433832 105 + 2.182 2.277 0.318
ENSG00000122729 E025 200.0202713 0.0002832696 1.008286e-02 2.896475e-02 9 32434559 32434701 143 + 2.249 2.329 0.269
ENSG00000122729 E026 228.9845108 0.0006736851 6.332886e-03 1.948842e-02 9 32436250 32436397 148 + 2.304 2.388 0.280
ENSG00000122729 E027 183.9280669 0.0026082927 8.447212e-05 4.491941e-04 9 32440465 32440587 123 + 2.164 2.315 0.505
ENSG00000122729 E028 248.4249698 0.0002537928 1.705155e-08 2.013692e-07 9 32448896 32449081 186 + 2.300 2.447 0.490
ENSG00000122729 E029 729.2728001 0.0021755952 1.205078e-10 2.098028e-09 9 32449998 32454769 4772 + 2.754 2.912 0.523