ENSG00000122678

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000242248 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding protein_coding 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 2.7624602 3.640968 2.0047283 0.2789679 0.21154914 -0.857693811 0.21656667 0.21773333 0.22090000 0.003166667 1.000000e+00 5.798759e-05 FALSE  
ENST00000395831 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding protein_coding 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 1.2201057 2.315748 0.5356091 0.3479027 0.06972789 -2.091754933 0.08611250 0.13536667 0.05740000 -0.077966667 5.798759e-05 5.798759e-05 FALSE  
ENST00000430942 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding nonsense_mediated_decay 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 0.9390275 1.614856 0.4837214 0.2732522 0.04852027 -1.718542925 0.06608333 0.09390000 0.05330000 -0.040600000 7.034180e-02 5.798759e-05 TRUE  
ENST00000435068 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding nonsense_mediated_decay 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 2.3741045 3.308169 1.2400375 0.5388182 0.09599100 -1.408416007 0.17595000 0.19280000 0.13423333 -0.058566667 5.259048e-02 5.798759e-05 FALSE  
ENST00000458246 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding nonsense_mediated_decay 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 0.9795978 1.146218 0.7751333 0.1551260 0.07302215 -0.558403827 0.07950833 0.07066667 0.08486667 0.014200000 7.969493e-01 5.798759e-05 TRUE  
ENST00000467607 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding retained_intron 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 0.8776748 1.044626 1.1099562 1.0446263 0.61919960 0.086710517 0.06283333 0.05020000 0.11283333 0.062633333 5.160251e-01 5.798759e-05 FALSE  
ENST00000492312 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding retained_intron 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 0.6513521 0.260115 0.4386930 0.1614581 0.14851389 0.732155176 0.05278333 0.01453333 0.04573333 0.031200000 3.734579e-01 5.798759e-05 FALSE  
ENST00000492605 ENSG00000122678 HEK293_OSMI2_2hA HEK293_TMG_2hB POLM protein_coding retained_intron 13.0361 16.99819 9.2688 2.161949 0.8375404 -0.8742196 1.5330321 1.380020 1.3772800 0.2136300 0.19479854 -0.002846272 0.12264167 0.08070000 0.14733333 0.066633333 4.296081e-03 5.798759e-05 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122678 E001 14.6111118 0.0012018693 2.037486e-06 1.582338e-05 7 44072062 44072246 185 - 1.402 0.990 -1.465
ENSG00000122678 E002 6.7179111 0.0026448626 6.515874e-02 1.353011e-01 7 44072247 44072249 3 - 0.996 0.773 -0.855
ENSG00000122678 E003 10.4573444 0.0027693620 5.307737e-02 1.146208e-01 7 44072250 44072255 6 - 1.142 0.940 -0.738
ENSG00000122678 E004 11.8924030 0.0089715869 4.456593e-01 5.872042e-01 7 44072256 44072261 6 - 1.110 1.028 -0.296
ENSG00000122678 E005 299.0563204 0.0048995048 1.684660e-02 4.459200e-02 7 44072262 44072977 716 - 2.347 2.445 0.326
ENSG00000122678 E006 63.9071174 0.0004408586 1.418076e-01 2.505182e-01 7 44072978 44072998 21 - 1.694 1.771 0.261
ENSG00000122678 E007 87.3930743 0.0003354132 2.704640e-02 6.608957e-02 7 44072999 44073075 77 - 1.812 1.910 0.333
ENSG00000122678 E008 68.5433577 0.0009785879 5.220712e-02 1.130819e-01 7 44073076 44073138 63 - 1.711 1.812 0.339
ENSG00000122678 E009 40.0499200 0.0005651035 1.751576e-01 2.948833e-01 7 44073139 44073145 7 - 1.487 1.576 0.304
ENSG00000122678 E010 72.3481411 0.0005227325 1.964181e-01 3.217510e-01 7 44073146 44073217 72 - 1.759 1.823 0.218
ENSG00000122678 E011 112.6548828 0.0013866830 5.376530e-01 6.691780e-01 7 44073218 44073377 160 - 1.971 2.001 0.101
ENSG00000122678 E012 27.5705078 0.0007663921 3.750347e-01 5.194490e-01 7 44073378 44073430 53 - 1.435 1.375 -0.206
ENSG00000122678 E013 1.3757300 0.0108072798 3.594329e-03 1.199399e-02 7 44073431 44073433 3 - 0.650 0.155 -3.016
ENSG00000122678 E014 74.1541740 0.0003878333 5.763209e-01 7.017865e-01 7 44073625 44073708 84 - 1.791 1.821 0.099
ENSG00000122678 E015 76.6557558 0.0004450433 6.722123e-01 7.782622e-01 7 44073783 44073861 79 - 1.812 1.834 0.077
ENSG00000122678 E016 107.7257353 0.0003114689 7.140394e-01 8.103603e-01 7 44073862 44074025 164 - 1.961 1.979 0.059
ENSG00000122678 E017 16.1387703 0.0011174009 4.145263e-02 9.372249e-02 7 44074026 44074130 105 - 1.297 1.121 -0.622
ENSG00000122678 E018 33.2905773 0.0006681248 9.230776e-01 9.554976e-01 7 44074131 44074140 10 - 1.473 1.470 -0.009
ENSG00000122678 E019 41.5579747 0.0005512787 9.887840e-01 9.970309e-01 7 44074141 44074184 44 - 1.564 1.568 0.016
ENSG00000122678 E020 41.6818115 0.0006448491 9.941461e-02 1.895912e-01 7 44074185 44074220 36 - 1.494 1.600 0.362
ENSG00000122678 E021 27.3502024 0.0007471631 2.400940e-01 3.742603e-01 7 44074221 44074233 13 - 1.328 1.420 0.318
ENSG00000122678 E022 36.3355435 0.0006324675 4.444789e-01 5.860391e-01 7 44074234 44074397 164 - 1.473 1.526 0.183
ENSG00000122678 E023 65.1228104 0.0004137361 2.336727e-01 3.667594e-01 7 44074398 44074522 125 - 1.716 1.778 0.210
ENSG00000122678 E024 29.1470350 0.0188408060 3.594546e-01 5.037848e-01 7 44074523 44074530 8 - 1.357 1.446 0.309
ENSG00000122678 E025 52.3391216 0.0025530989 1.565394e-07 1.531577e-06 7 44075719 44076508 790 - 1.850 1.570 -0.947
ENSG00000122678 E026 36.2133100 0.0006036397 1.868716e-01 3.098042e-01 7 44076509 44076576 68 - 1.569 1.491 -0.268
ENSG00000122678 E027 29.6440498 0.0007189038 9.409752e-02 1.815362e-01 7 44076577 44076629 53 - 1.507 1.398 -0.377
ENSG00000122678 E028 15.2456895 0.0095751081 4.147128e-01 5.581247e-01 7 44076869 44077026 158 - 1.213 1.135 -0.277
ENSG00000122678 E029 6.6969043 0.0026166424 4.392763e-01 5.810300e-01 7 44078322 44078350 29 - 0.899 0.803 -0.370
ENSG00000122678 E030 9.9903136 0.0017102868 1.241505e-01 2.258999e-01 7 44078351 44078438 88 - 1.093 0.930 -0.601
ENSG00000122678 E031 6.1635211 0.0028883524 3.322421e-02 7.824650e-02 7 44078439 44078465 27 - 0.973 0.705 -1.046
ENSG00000122678 E032 8.9218835 0.0019092378 6.474169e-02 1.346226e-01 7 44078466 44078553 88 - 1.075 0.871 -0.759
ENSG00000122678 E033 10.6736099 0.0015972520 5.772145e-04 2.449247e-03 7 44078554 44078647 94 - 1.239 0.895 -1.252
ENSG00000122678 E034 9.7181014 0.0429164984 4.210353e-04 1.855060e-03 7 44078648 44078739 92 - 1.275 0.790 -1.788
ENSG00000122678 E035 27.7487586 0.0007782412 1.611618e-01 2.766197e-01 7 44078740 44078755 16 - 1.473 1.379 -0.324
ENSG00000122678 E036 50.0004370 0.0004480389 6.773468e-01 7.820895e-01 7 44078756 44078811 56 - 1.663 1.644 -0.064
ENSG00000122678 E037 50.5414693 0.0004928240 8.711546e-01 9.211694e-01 7 44079571 44079636 66 - 1.644 1.657 0.044
ENSG00000122678 E038 58.1933533 0.0005019707 8.487698e-01 9.061278e-01 7 44079637 44079732 96 - 1.699 1.713 0.047
ENSG00000122678 E039 27.3466693 0.0031202304 9.821917e-01 9.929097e-01 7 44079733 44079741 9 - 1.385 1.392 0.024
ENSG00000122678 E040 2.1046358 0.3260347478 6.848930e-01 7.877203e-01 7 44079742 44079756 15 - 0.542 0.427 -0.569
ENSG00000122678 E041 0.8814403 0.0141493497 1.196591e-01 2.194848e-01 7 44079815 44079860 46 - 0.000 0.316 10.563
ENSG00000122678 E042 57.0833786 0.0004519584 3.463866e-01 4.905268e-01 7 44079861 44079959 99 - 1.663 1.716 0.181
ENSG00000122678 E043 0.4741261 0.0210424827 1.958737e-01 3.210702e-01 7 44080365 44080471 107 - 0.299 0.084 -2.207
ENSG00000122678 E044 63.4336547 0.0107987585 2.441037e-01 3.790225e-01 7 44080733 44080916 184 - 1.808 1.734 -0.250
ENSG00000122678 E045 49.7587246 0.0082827741 2.255765e-01 3.571083e-01 7 44082251 44082530 280 - 1.703 1.627 -0.257