ENSG00000122643

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000409787 ENSG00000122643 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C3A protein_coding protein_coding 23.9424 13.30838 32.54035 0.4195224 1.1507 1.289254 1.211437 0.3422331 1.696234 0.09765543 0.1161473 2.276213 0.05026667 0.02590000 0.05203333 0.02613333 8.621020e-02 2.586222e-24 FALSE TRUE
ENST00000456458 ENSG00000122643 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C3A protein_coding nonsense_mediated_decay 23.9424 13.30838 32.54035 0.4195224 1.1507 1.289254 3.068059 0.7742284 4.698341 0.16592254 0.9046226 2.585873 0.12247083 0.05853333 0.14316667 0.08463333 2.405799e-02 2.586222e-24 FALSE TRUE
ENST00000610140 ENSG00000122643 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C3A protein_coding protein_coding 23.9424 13.30838 32.54035 0.4195224 1.1507 1.289254 17.747101 9.3367515 23.353636 0.39320999 1.0705784 1.321728 0.72539167 0.70290000 0.71883333 0.01593333 9.300489e-01 2.586222e-24 FALSE TRUE
ENST00000643244 ENSG00000122643 HEK293_OSMI2_2hA HEK293_TMG_2hB NT5C3A protein_coding protein_coding 23.9424 13.30838 32.54035 0.4195224 1.1507 1.289254 0.563776 2.2587671 0.000000 0.45727551 0.0000000 -7.825765 0.04832500 0.16806667 0.00000000 -0.16806667 2.586222e-24 2.586222e-24 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122643 E001 189.9236232 0.0036252017 2.711778e-12 6.206066e-11 7 33014130 33014450 321 - 2.143 2.423 0.932
ENSG00000122643 E002 228.5816286 0.0033237784 1.693419e-08 2.001080e-07 7 33014451 33014719 269 - 2.253 2.467 0.712
ENSG00000122643 E003 132.1348945 0.0017666000 2.156517e-04 1.031134e-03 7 33014720 33014831 112 - 2.040 2.197 0.525
ENSG00000122643 E004 193.4977543 0.0002119699 3.251287e-01 4.685514e-01 7 33015670 33015870 201 - 2.250 2.300 0.169
ENSG00000122643 E005 12.8696267 0.0014750477 7.122724e-01 8.090637e-01 7 33015871 33015926 56 - 1.095 1.154 0.212
ENSG00000122643 E006 7.1309364 0.0023077398 6.064899e-01 7.266267e-01 7 33017204 33017438 235 - 0.910 0.868 -0.160
ENSG00000122643 E007 196.9728049 0.0002040982 2.496555e-02 6.180122e-02 7 33017439 33017601 163 - 2.287 2.254 -0.110
ENSG00000122643 E008 151.5519632 0.0002319844 1.366763e-02 3.746809e-02 7 33019635 33019724 90 - 2.180 2.132 -0.162
ENSG00000122643 E009 132.0402920 0.0002457104 2.214779e-03 7.889127e-03 7 33021272 33021357 86 - 2.128 2.054 -0.248
ENSG00000122643 E010 2.3754675 0.0064531968 9.558873e-04 3.807771e-03 7 33021825 33022052 228 - 0.649 0.000 -13.608
ENSG00000122643 E011 98.5702111 0.0002768687 5.717812e-03 1.786552e-02 7 33022053 33022099 47 - 2.003 1.924 -0.266
ENSG00000122643 E012 0.1451727 0.0433415054 1.000000e+00   7 33023719 33024038 320 - 0.085 0.000 -9.608
ENSG00000122643 E013 101.6912353 0.0002994720 1.091552e-03 4.275463e-03 7 33024039 33024080 42 - 2.021 1.924 -0.327
ENSG00000122643 E014 97.2211470 0.0003536193 5.106502e-06 3.627068e-05 7 33024081 33024108 28 - 2.018 1.864 -0.516
ENSG00000122643 E015 137.7198651 0.0003129695 9.303531e-07 7.772650e-06 7 33026817 33026915 99 - 2.163 2.027 -0.453
ENSG00000122643 E016 0.1451727 0.0433415054 1.000000e+00   7 33026916 33027033 118 - 0.085 0.000 -9.608
ENSG00000122643 E017 0.0000000       7 33029231 33029628 398 -      
ENSG00000122643 E018 0.5891098 0.0183152715 1.682408e-01 2.859130e-01 7 33029629 33029709 81 - 0.270 0.000 -11.608
ENSG00000122643 E019 0.1451727 0.0433415054 1.000000e+00   7 33029710 33029714 5 - 0.085 0.000 -9.608
ENSG00000122643 E020 18.5609918 0.0102777615 1.460949e-02 3.961475e-02 7 33035934 33035988 55 - 1.333 1.107 -0.798
ENSG00000122643 E021 0.3268771 0.0315914312 5.745596e-01   7 33040872 33040910 39 - 0.085 0.173 1.186
ENSG00000122643 E022 106.3277452 0.0003309377 1.688241e-07 1.641427e-06 7 33062568 33062796 229 - 2.061 1.890 -0.577