ENSG00000122550

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000322275 ENSG00000122550 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL7 protein_coding protein_coding 15.49309 7.928853 23.03 0.8485006 1.046704 1.537138 7.364861 5.4812547 11.056302 0.7270392 0.7642204 1.010965 0.5194042 0.68786667 0.4809333 -0.2069333 0.0005945921 0.0005945921 FALSE TRUE
ENST00000521082 ENSG00000122550 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL7 protein_coding nonsense_mediated_decay 15.49309 7.928853 23.03 0.8485006 1.046704 1.537138 1.964109 0.8132952 3.070564 0.2020640 0.1998139 1.903713 0.1228958 0.09986667 0.1342667 0.0344000 0.5184545494 0.0005945921 FALSE TRUE
MSTRG.29565.7 ENSG00000122550 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL7 protein_coding   15.49309 7.928853 23.03 0.8485006 1.046704 1.537138 4.119811 1.2003924 5.980897 0.1254266 0.1674508 2.307297 0.2425333 0.15266667 0.2606667 0.1080000 0.0032710470 0.0005945921 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122550 E001 6.1876040 0.0097824476 9.676976e-01 9.838169e-01 7 23105758 23105784 27 + 0.825 0.819 -0.025
ENSG00000122550 E002 6.5573424 0.0064927843 6.457906e-01 7.578614e-01 7 23105785 23105789 5 + 0.825 0.887 0.238
ENSG00000122550 E003 44.1069789 0.0050265485 9.959863e-02 1.898638e-01 7 23105790 23105873 84 + 1.584 1.689 0.358
ENSG00000122550 E004 116.7590180 0.0003005273 8.739688e-02 1.711927e-01 7 23105874 23106116 243 + 2.013 2.075 0.206
ENSG00000122550 E005 59.0579692 0.0011374453 1.286900e-01 2.323314e-01 7 23106117 23106146 30 + 1.719 1.799 0.269
ENSG00000122550 E006 0.2934659 0.0283619907 4.303680e-01   7 23106295 23106297 3 + 0.075 0.193 1.558
ENSG00000122550 E007 2.6571889 0.0056356407 7.130332e-01 8.095907e-01 7 23106298 23106655 358 + 0.515 0.579 0.297
ENSG00000122550 E008 3.1626368 0.0053300510 6.458257e-01 7.578980e-01 7 23106656 23106794 139 + 0.563 0.639 0.338
ENSG00000122550 E009 10.1317046 0.0018958902 5.580265e-01 6.863278e-01 7 23117834 23117964 131 + 1.036 0.972 -0.235
ENSG00000122550 E010 100.7028693 0.0003582827 3.183316e-03 1.080673e-02 7 23123777 23123879 103 + 1.936 2.047 0.374
ENSG00000122550 E011 106.3219107 0.0003372445 5.272069e-03 1.667269e-02 7 23124688 23124781 94 + 1.960 2.063 0.344
ENSG00000122550 E012 0.1515154 0.0431901371 1.000000e+00   7 23124980 23125047 68 + 0.075 0.000 -8.647
ENSG00000122550 E013 124.3804750 0.0004862378 9.510614e-03 2.756329e-02 7 23125048 23125172 125 + 2.031 2.121 0.301
ENSG00000122550 E014 0.1515154 0.0431901371 1.000000e+00   7 23125173 23125202 30 + 0.075 0.000 -8.647
ENSG00000122550 E015 41.5544909 0.0130346465 1.861441e-04 9.042162e-04 7 23125761 23125799 39 + 1.482 1.780 1.011
ENSG00000122550 E016 51.0877809 0.0005681938 6.087254e-09 7.822629e-08 7 23125800 23126049 250 + 1.573 1.867 0.996
ENSG00000122550 E017 74.3496288 0.0003357238 2.816537e-05 1.686051e-04 7 23140769 23140944 176 + 1.885 1.686 -0.674
ENSG00000122550 E018 53.4263300 0.0015694105 1.287365e-02 3.562119e-02 7 23143851 23143928 78 + 1.730 1.585 -0.492
ENSG00000122550 E019 56.9761600 0.0004269323 3.431491e-02 8.036166e-02 7 23143929 23144025 97 + 1.754 1.644 -0.373
ENSG00000122550 E020 62.8776770 0.0006362467 1.611519e-04 7.971422e-04 7 23152067 23152209 143 + 1.813 1.615 -0.671
ENSG00000122550 E021 6.8620306 0.0025155667 2.776069e-02 6.754228e-02 7 23155991 23156168 178 + 0.773 1.043 1.028
ENSG00000122550 E022 47.8557140 0.0049133445 1.009331e-05 6.699884e-05 7 23165698 23165874 177 + 1.717 1.399 -1.087
ENSG00000122550 E023 22.8385788 0.0008002580 2.882550e-03 9.918456e-03 7 23165875 23165938 64 + 1.399 1.141 -0.905
ENSG00000122550 E024 61.0944004 0.0003876392 7.875024e-06 5.357680e-05 7 23167836 23168037 202 + 1.811 1.572 -0.810
ENSG00000122550 E025 0.0000000       7 23168038 23168102 65 +      
ENSG00000122550 E026 0.1515154 0.0431901371 1.000000e+00   7 23172789 23172947 159 + 0.075 0.000 -8.647
ENSG00000122550 E027 54.8257119 0.0004485963 6.569264e-04 2.740948e-03 7 23172948 23173045 98 + 1.754 1.565 -0.640
ENSG00000122550 E028 251.2678423 0.0024358904 4.655169e-01 6.052112e-01 7 23174015 23177914 3900 + 2.368 2.345 -0.076