ENSG00000122376

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298784 ENSG00000122376 HEK293_OSMI2_2hA HEK293_TMG_2hB SHLD2 protein_coding protein_coding 17.16187 4.287863 30.34885 0.4239781 0.9366559 2.820426 4.529886 0.000000 8.6804803 0.0000000 0.3280083 9.763292 0.2171500 0.0000 0.28610000 0.28610000 4.310740e-30 4.31074e-30 FALSE TRUE
ENST00000298786 ENSG00000122376 HEK293_OSMI2_2hA HEK293_TMG_2hB SHLD2 protein_coding protein_coding 17.16187 4.287863 30.34885 0.4239781 0.9366559 2.820426 7.798608 2.669614 16.0927679 0.3908253 0.3888241 2.587212 0.3764500 0.6349 0.53086667 -0.10403333 6.337377e-01 4.31074e-30 FALSE TRUE
MSTRG.4324.4 ENSG00000122376 HEK293_OSMI2_2hA HEK293_TMG_2hB SHLD2 protein_coding   17.16187 4.287863 30.34885 0.4239781 0.9366559 2.820426 0.376406 1.216966 0.0000000 0.4807471 0.0000000 -6.938951 0.0820250 0.2698 0.00000000 -0.26980000 6.867600e-14 4.31074e-30 FALSE TRUE
MSTRG.4324.5 ENSG00000122376 HEK293_OSMI2_2hA HEK293_TMG_2hB SHLD2 protein_coding   17.16187 4.287863 30.34885 0.4239781 0.9366559 2.820426 1.882824 0.000000 0.7583906 0.0000000 0.3874404 6.263768 0.1857625 0.0000 0.02423333 0.02423333 5.044717e-01 4.31074e-30 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122376 E001 14.6391355 0.0011983354 2.352006e-01 3.685956e-01 10 87094161 87094788 628 + 1.059 0.960 -0.367
ENSG00000122376 E002 0.4482035 0.0263809781 1.000000e+00 1.000000e+00 10 87095157 87095181 25 + 0.131 0.001 -7.079
ENSG00000122376 E003 1.4780283 0.0091941290 7.809174e-01 8.592331e-01 10 87095182 87095190 9 + 0.313 0.279 -0.231
ENSG00000122376 E004 1.4780283 0.0091941290 7.809174e-01 8.592331e-01 10 87095191 87095193 3 + 0.313 0.279 -0.231
ENSG00000122376 E005 2.2249963 0.0063662840 4.299508e-01 5.725950e-01 10 87095194 87095195 2 + 0.422 0.279 -0.868
ENSG00000122376 E006 3.4094449 0.0047260088 1.605360e-01 2.758454e-01 10 87095196 87095203 8 + 0.554 0.279 -1.520
ENSG00000122376 E007 4.5876646 0.0034023611 5.835159e-02 1.237999e-01 10 87095204 87095205 2 + 0.656 0.279 -1.968
ENSG00000122376 E008 22.5958834 0.0056506515 5.772088e-02 1.227223e-01 10 87095206 87095248 43 + 1.239 1.072 -0.595
ENSG00000122376 E009 41.9800597 0.0044806342 4.379937e-02 9.802313e-02 10 87096934 87096989 56 + 1.491 1.371 -0.412
ENSG00000122376 E010 8.9467456 0.0157472712 5.474176e-01 6.774757e-01 10 87104682 87104962 281 + 0.859 0.807 -0.202
ENSG00000122376 E011 185.7796835 0.0006828107 4.397240e-11 8.234914e-10 10 87151350 87151832 483 + 2.132 1.910 -0.744
ENSG00000122376 E012 271.7341469 0.0011894753 4.541360e-07 4.050217e-06 10 87151833 87152879 1047 + 2.285 2.171 -0.381
ENSG00000122376 E013 106.3450912 0.0002672669 3.907503e-01 5.347110e-01 10 87158048 87158155 108 + 1.866 1.885 0.063
ENSG00000122376 E014 147.5469301 0.0002343876 1.366882e-02 3.746896e-02 10 87170478 87170672 195 + 2.016 1.972 -0.145
ENSG00000122376 E015 106.0228138 0.0002856959 9.157827e-02 1.775984e-01 10 87170840 87170974 135 + 1.871 1.848 -0.079
ENSG00000122376 E016 0.8042123 0.1002868227 6.462784e-01 7.582367e-01 10 87173063 87173154 92 + 0.167 0.281 0.952
ENSG00000122376 E017 78.5378588 0.0003841112 2.796371e-01 4.193997e-01 10 87175889 87176095 207 + 1.719 1.836 0.397
ENSG00000122376 E018 85.1648713 0.0005248825 5.857561e-01 7.093891e-01 10 87180075 87180125 51 + 1.759 1.848 0.300
ENSG00000122376 E019 115.4851568 0.0003239201 1.739985e-02 4.582336e-02 10 87180126 87180303 178 + 1.876 2.038 0.546
ENSG00000122376 E020 105.7582134 0.0006965278 6.258971e-03 1.929104e-02 10 87187085 87187200 116 + 1.836 2.020 0.618
ENSG00000122376 E021 4.8048288 0.0169524174 5.760235e-01 7.015318e-01 10 87187201 87187269 69 + 0.632 0.570 -0.277
ENSG00000122376 E022 270.6952399 0.0306073856 6.253669e-04 2.626275e-03 10 87190484 87191465 982 + 2.208 2.542 1.114