ENSG00000122335

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000607071 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding nonsense_mediated_decay 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.22101601 0.11379093 0.8773964 0.11379093 0.17064089 2.8416731 0.026295833 0.03983333 0.09096667 0.051133333 3.718711e-01 5.116026e-05 FALSE TRUE
ENST00000642244 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding protein_coding 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.58837773 0.17429170 0.6622277 0.09308304 0.66222769 1.8669589 0.074687500 0.06646667 0.06940000 0.002933333 4.789613e-01 5.116026e-05 FALSE TRUE
ENST00000642903 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding protein_coding 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.39662189 0.03502129 0.8049879 0.03502129 0.40778032 4.1780993 0.047991667 0.01413333 0.08313333 0.069000000 6.174822e-01 5.116026e-05 FALSE TRUE
ENST00000644972 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding protein_coding 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.35066842 0.00000000 0.6300159 0.00000000 0.09020257 6.0000359 0.055050000 0.00000000 0.06536667 0.065366667 5.116026e-05 5.116026e-05 FALSE TRUE
ENST00000646208 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding protein_coding 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.16833433 0.37092154 0.4194638 0.37092154 0.41946376 0.1730425 0.038491667 0.10553333 0.04310000 -0.062433333 8.688252e-01 5.116026e-05 FALSE TRUE
ENST00000646410 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding protein_coding 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.07728363 0.00000000 0.5246664 0.00000000 0.27555765 5.7405672 0.008008333 0.00000000 0.05473333 0.054733333 3.847036e-01 5.116026e-05 FALSE TRUE
ENST00000647468 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding protein_coding 5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 2.90239591 1.95287031 4.3124992 0.10533892 0.56553646 1.1389008 0.558687500 0.67320000 0.44803333 -0.225166667 1.346222e-01 5.116026e-05 FALSE TRUE
MSTRG.29242.7 ENSG00000122335 HEK293_OSMI2_2hA HEK293_TMG_2hB SERAC1 protein_coding   5.963365 2.949629 9.634116 0.3029748 0.05581981 1.704233 0.60806565 0.00000000 0.4547436 0.00000000 0.45474358 5.5383630 0.066595833 0.00000000 0.04670000 0.046700000 1.000000e+00 5.116026e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122335 E001 2.5390952 0.0575972863 6.489178e-02 1.348584e-01 6 158109519 158109534 16 - 0.380 0.722 1.613
ENSG00000122335 E002 2.8356816 0.0063950245 9.726559e-03 2.809030e-02 6 158109535 158109540 6 - 0.380 0.800 1.925
ENSG00000122335 E003 20.9139513 0.0212385923 9.746149e-03 2.813830e-02 6 158109541 158109690 150 - 1.204 1.495 1.012
ENSG00000122335 E004 12.7376815 0.0258060528 1.992873e-01 3.253454e-01 6 158109691 158109692 2 - 1.049 1.235 0.668
ENSG00000122335 E005 72.0936145 0.0230032533 7.775403e-05 4.174536e-04 6 158109693 158110224 532 - 1.690 2.049 1.211
ENSG00000122335 E006 48.9975398 0.0005235668 6.985377e-08 7.309355e-07 6 158110225 158110598 374 - 1.560 1.851 0.986
ENSG00000122335 E007 15.2868907 0.0011806409 1.454767e-03 5.489191e-03 6 158110599 158110619 21 - 1.071 1.367 1.047
ENSG00000122335 E008 12.2817108 0.0015582710 3.272729e-02 7.725483e-02 6 158110620 158110626 7 - 1.016 1.241 0.809
ENSG00000122335 E009 22.2073221 0.0008465588 2.418392e-05 1.472086e-04 6 158110627 158110667 41 - 1.209 1.537 1.139
ENSG00000122335 E010 47.5433177 0.0004966501 1.363575e-08 1.639120e-07 6 158110668 158110871 204 - 1.536 1.845 1.048
ENSG00000122335 E011 121.8844005 0.0043853859 2.417884e-01 3.763018e-01 6 158110872 158111502 631 - 2.037 2.103 0.222
ENSG00000122335 E012 23.4099600 0.0010464808 1.443158e-01 2.538834e-01 6 158111503 158113448 1946 - 1.387 1.291 -0.331
ENSG00000122335 E013 57.1802926 0.0010384428 7.743675e-02 1.553478e-01 6 158113449 158113592 144 - 1.755 1.683 -0.244
ENSG00000122335 E014 0.2998086 0.0283981547 4.959435e-01   6 158114471 158114534 64 - 0.079 0.185 1.407
ENSG00000122335 E015 0.7739347 0.0148542758 5.796899e-01 7.045359e-01 6 158114535 158114592 58 - 0.204 0.314 0.824
ENSG00000122335 E016 1.5823117 0.0091752397 1.552060e-01 2.686608e-01 6 158114593 158114730 138 - 0.301 0.562 1.409
ENSG00000122335 E017 3.7458964 0.0114880812 7.271285e-01 8.199494e-01 6 158114731 158114788 58 - 0.662 0.621 -0.176
ENSG00000122335 E018 36.3932860 0.0006048006 3.726996e-02 8.598446e-02 6 158114789 158114825 37 - 1.577 1.464 -0.386
ENSG00000122335 E019 58.1094719 0.0003961664 1.150979e-02 3.237221e-02 6 158114826 158114971 146 - 1.772 1.664 -0.366
ENSG00000122335 E020 49.6423394 0.0010906225 2.114129e-01 3.401519e-01 6 158116185 158116282 98 - 1.688 1.638 -0.169
ENSG00000122335 E021 2.6021096 0.0071092905 1.037304e-01 1.961904e-01 6 158116283 158117531 1249 - 0.602 0.314 -1.497
ENSG00000122335 E022 0.4470576 0.0213176081 8.509033e-01 9.075666e-01 6 158117532 158117575 44 - 0.146 0.185 0.408
ENSG00000122335 E023 0.8930711 0.0543389817 5.245713e-01 6.580571e-01 6 158117576 158117726 151 - 0.301 0.184 -0.917
ENSG00000122335 E024 41.8205616 0.0005007556 6.388176e-01 7.523862e-01 6 158117727 158117821 95 - 1.606 1.599 -0.023
ENSG00000122335 E025 37.2050712 0.0005594597 6.844157e-02 1.407945e-01 6 158119029 158119120 92 - 1.581 1.487 -0.322
ENSG00000122335 E026 27.0935030 0.0008329140 2.478593e-01 3.834098e-01 6 158119121 158119170 50 - 1.440 1.377 -0.220
ENSG00000122335 E027 45.4634092 0.0006546928 5.198389e-02 1.126976e-01 6 158120425 158120575 151 - 1.666 1.575 -0.309
ENSG00000122335 E028 52.8517624 0.0004707505 1.690640e-03 6.253279e-03 6 158128108 158128270 163 - 1.743 1.593 -0.508
ENSG00000122335 E029 37.6711980 0.0005680498 1.483177e-03 5.582490e-03 6 158130373 158130486 114 - 1.608 1.423 -0.635
ENSG00000122335 E030 40.0165858 0.0056121239 2.440914e-02 6.066165e-02 6 158143056 158143184 129 - 1.622 1.479 -0.490
ENSG00000122335 E031 34.6461710 0.0182602752 1.053471e-02 3.005495e-02 6 158144299 158144420 122 - 1.581 1.347 -0.803
ENSG00000122335 E032 0.0000000       6 158145264 158145363 100 -      
ENSG00000122335 E033 3.6316396 0.0275608279 3.225245e-02 7.633749e-02 6 158145920 158146781 862 - 0.505 0.864 1.524
ENSG00000122335 E034 29.4457096 0.0009931728 1.255357e-01 2.279205e-01 6 158146782 158146869 88 - 1.476 1.386 -0.310
ENSG00000122335 E035 21.5868323 0.0009304820 2.510006e-01 3.871374e-01 6 158146870 158146913 44 - 1.342 1.267 -0.259
ENSG00000122335 E036 22.8876511 0.0072770104 8.657849e-02 1.699305e-01 6 158148865 158148954 90 - 1.383 1.243 -0.490
ENSG00000122335 E037 28.6361950 0.0008610514 6.529452e-01 7.635474e-01 6 158150453 158150563 111 - 1.443 1.431 -0.041
ENSG00000122335 E038 14.2672219 0.0013131826 2.376935e-01 3.714729e-01 6 158150564 158150587 24 - 1.181 1.084 -0.347
ENSG00000122335 E039 9.6752829 0.0018169335 2.756508e-01 4.149943e-01 6 158150588 158150589 2 - 1.033 0.926 -0.398
ENSG00000122335 E040 0.1472490 0.0440076235 1.000000e+00   6 158151039 158151129 91 - 0.079 0.000 -8.265
ENSG00000122335 E041 0.0000000       6 158152756 158152842 87 -      
ENSG00000122335 E042 12.0055061 0.0013997574 5.975431e-01 7.192774e-01 6 158155315 158155351 37 - 1.100 1.065 -0.127
ENSG00000122335 E043 26.1689799 0.0007707704 1.686325e-05 1.063479e-04 6 158158273 158158364 92 - 1.485 1.154 -1.156
ENSG00000122335 E044 1.2576127 0.0102978509 8.352275e-01 8.968967e-01 6 158158365 158159298 934 - 0.342 0.314 -0.175
ENSG00000122335 E045 2.1508993 0.0096922794 5.030931e-02 1.097138e-01 6 158160932 158162147 1216 - 0.556 0.185 -2.289
ENSG00000122335 E046 0.4439371 0.0215668936 3.604023e-01 5.047171e-01 6 158162148 158162152 5 - 0.204 0.000 -9.850
ENSG00000122335 E047 0.0000000       6 158166114 158166159 46 -      
ENSG00000122335 E048 22.7506515 0.0008342172 1.011076e-04 5.270050e-04 6 158168140 158168280 141 - 1.421 1.103 -1.119