ENSG00000122299

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000355758 ENSG00000122299 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H7A protein_coding protein_coding 21.78791 12.41399 34.09735 0.9465007 0.223666 1.456954 12.185043 4.6640479 21.191231 0.8120732 1.1195603 2.181404 0.51656250 0.37926667 0.62156667 0.242300000 5.097428e-02 9.689789e-09 FALSE TRUE
ENST00000570862 ENSG00000122299 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H7A protein_coding retained_intron 21.78791 12.41399 34.09735 0.9465007 0.223666 1.456954 1.460353 0.4303496 2.554673 0.2205742 0.6028379 2.542053 0.06352083 0.03303333 0.07476667 0.041733333 5.239247e-01 9.689789e-09   FALSE
ENST00000571405 ENSG00000122299 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H7A protein_coding nonsense_mediated_decay 21.78791 12.41399 34.09735 0.9465007 0.223666 1.456954 2.323747 4.7919959 1.411072 0.4120590 0.5117544 -1.756654 0.15825833 0.38690000 0.04146667 -0.345433333 6.315949e-06 9.689789e-09 FALSE TRUE
ENST00000575984 ENSG00000122299 HEK293_OSMI2_2hA HEK293_TMG_2hB ZC3H7A protein_coding protein_coding 21.78791 12.41399 34.09735 0.9465007 0.223666 1.456954 1.386037 0.6569664 1.859349 0.1094944 0.1541463 1.486850 0.06794167 0.05223333 0.05456667 0.002333333 9.690169e-01 9.689789e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122299 E001 0.1482932 0.0418925105 1.706969e-01   16 11750586 11750590 5 - 0.000 0.191 12.111
ENSG00000122299 E002 0.4448795 0.5618376441 7.775150e-02 1.558526e-01 16 11750591 11750592 2 - 0.000 0.408 13.655
ENSG00000122299 E003 0.7415677 0.2081271762 2.138429e-01 3.430624e-01 16 11750593 11750594 2 - 0.141 0.416 2.067
ENSG00000122299 E004 1.5563004 0.1562417382 7.719932e-02 1.550016e-01 16 11750595 11750596 2 - 0.248 0.627 2.072
ENSG00000122299 E005 463.2083043 0.0021876536 7.253911e-25 7.857242e-23 16 11750597 11751506 910 - 2.522 2.806 0.945
ENSG00000122299 E006 181.3870263 0.0012971434 2.517548e-03 8.815932e-03 16 11752669 11752832 164 - 2.184 2.299 0.384
ENSG00000122299 E007 0.9630129 0.0285861026 4.630063e-02 1.025764e-01 16 11752833 11753162 330 - 0.141 0.506 2.523
ENSG00000122299 E008 156.2634213 0.0002340096 4.661428e-04 2.030567e-03 16 11756237 11756321 85 - 2.116 2.239 0.412
ENSG00000122299 E009 94.3353231 0.0002995998 3.619290e-04 1.626453e-03 16 11756322 11756328 7 - 1.886 2.041 0.520
ENSG00000122299 E010 132.3633711 0.0004551414 2.550836e-02 6.292787e-02 16 11756329 11756370 42 - 2.056 2.148 0.310
ENSG00000122299 E011 180.3014162 0.0036922933 2.436246e-01 3.784321e-01 16 11758431 11758539 109 - 2.199 2.264 0.217
ENSG00000122299 E012 40.1021590 0.0005341930 8.175903e-01 8.846613e-01 16 11758540 11759850 1311 - 1.571 1.574 0.009
ENSG00000122299 E013 115.7771061 0.0101417540 5.168187e-01 6.513291e-01 16 11761406 11761467 62 - 2.035 2.015 -0.069
ENSG00000122299 E014 111.5884835 0.0055579119 7.579538e-01 8.426313e-01 16 11761468 11761511 44 - 2.012 2.015 0.008
ENSG00000122299 E015 8.4387377 0.0153454925 4.835741e-02 1.062571e-01 16 11761512 11761662 151 - 1.001 0.734 -1.027
ENSG00000122299 E016 97.5949049 0.0002936903 1.665340e-01 2.836970e-01 16 11761910 11761947 38 - 1.931 2.002 0.237
ENSG00000122299 E017 113.0674128 0.0024587305 1.321268e-01 2.370632e-01 16 11761948 11762043 96 - 1.991 2.072 0.272
ENSG00000122299 E018 97.9511553 0.0019026402 1.575479e-01 2.718502e-01 16 11762671 11762747 77 - 1.929 2.007 0.262
ENSG00000122299 E019 5.8433237 0.0112881281 3.051000e-01 4.471422e-01 16 11762748 11763229 482 - 0.829 0.688 -0.569
ENSG00000122299 E020 146.9043196 0.0003281596 5.185308e-02 1.124590e-01 16 11763478 11763659 182 - 2.102 2.182 0.265
ENSG00000122299 E021 1.0716550 0.0121811594 4.409016e-01 5.825440e-01 16 11764965 11765052 88 - 0.333 0.191 -1.058
ENSG00000122299 E022 102.7800566 0.0004321082 6.742447e-01 7.797527e-01 16 11765053 11765153 101 - 1.964 1.997 0.112
ENSG00000122299 E023 60.6391038 0.0003723509 6.593233e-01 7.681625e-01 16 11765489 11765516 28 - 1.736 1.775 0.131
ENSG00000122299 E024 88.4809436 0.0003389631 2.922926e-01 4.332454e-01 16 11765517 11765574 58 - 1.915 1.888 -0.092
ENSG00000122299 E025 116.3909404 0.0003681075 9.038308e-01 9.428130e-01 16 11765575 11765685 111 - 2.022 2.034 0.043
ENSG00000122299 E026 61.4693335 0.0003975388 4.963695e-01 6.330581e-01 16 11767417 11767436 20 - 1.759 1.742 -0.058
ENSG00000122299 E027 137.1040609 0.0012561815 7.622570e-04 3.122860e-03 16 11767437 11767578 142 - 2.126 2.015 -0.372
ENSG00000122299 E028 154.3234067 0.0002293832 4.743808e-09 6.218517e-08 16 11768315 11768501 187 - 2.196 2.016 -0.602
ENSG00000122299 E029 0.7363589 0.0155791519 1.635633e-01 2.797682e-01 16 11768502 11768521 20 - 0.292 0.000 -11.704
ENSG00000122299 E030 79.6922022 0.0040359183 1.206419e-04 6.169591e-04 16 11769031 11769095 65 - 1.915 1.716 -0.671
ENSG00000122299 E031 141.8608455 0.0002755551 8.882921e-11 1.581133e-09 16 11770783 11770987 205 - 2.166 1.951 -0.720
ENSG00000122299 E032 166.7524646 0.0078583706 6.591219e-03 2.016287e-02 16 11774236 11774519 284 - 2.215 2.094 -0.406
ENSG00000122299 E033 65.2565355 0.0073954285 6.698830e-01 7.765240e-01 16 11774980 11775013 34 - 1.778 1.772 -0.021
ENSG00000122299 E034 1.8186230 0.0086410951 1.144158e-01 2.119856e-01 16 11775527 11775632 106 - 0.493 0.191 -1.933
ENSG00000122299 E035 60.1909917 0.0050983470 4.483064e-01 5.895972e-01 16 11776320 11776358 39 - 1.750 1.722 -0.092
ENSG00000122299 E036 85.8028451 0.0003544703 3.012256e-03 1.030245e-02 16 11776452 11776532 81 - 1.927 1.814 -0.383
ENSG00000122299 E037 52.6065483 0.0004229576 2.382028e-03 8.404129e-03 16 11776751 11776757 7 - 1.729 1.574 -0.527
ENSG00000122299 E038 101.9025525 0.0003704694 1.959146e-05 1.218018e-04 16 11776758 11776909 152 - 2.012 1.852 -0.536
ENSG00000122299 E039 65.1073728 0.0004012855 2.709685e-04 1.261623e-03 16 11779166 11779239 74 - 1.821 1.650 -0.578
ENSG00000122299 E040 47.1673641 0.0004670303 1.507151e-02 4.064994e-02 16 11779240 11779261 22 - 1.674 1.547 -0.431
ENSG00000122299 E041 53.6495855 0.0004636661 9.288274e-04 3.715300e-03 16 11779262 11779289 28 - 1.738 1.567 -0.580
ENSG00000122299 E042 67.6456857 0.0004795288 8.563845e-06 5.779611e-05 16 11779290 11779363 74 - 1.846 1.633 -0.721
ENSG00000122299 E043 51.5334742 0.0021858772 1.479301e-04 7.380803e-04 16 11781425 11781464 40 - 1.732 1.512 -0.747
ENSG00000122299 E044 64.1566438 0.0004698134 2.264431e-06 1.740535e-05 16 11782287 11782388 102 - 1.828 1.593 -0.798
ENSG00000122299 E045 0.2214452 0.0373313062 1.696131e-01   16 11782389 11782552 164 - 0.000 0.191 12.165
ENSG00000122299 E046 0.0000000       16 11782864 11783034 171 -      
ENSG00000122299 E047 14.2173756 0.0226025935 1.893839e-02 4.919676e-02 16 11796219 11796415 197 - 1.040 1.329 1.030
ENSG00000122299 E048 0.4417591 0.1462413877 1.829474e-01 3.048780e-01 16 11796416 11796527 112 - 0.076 0.320 2.505
ENSG00000122299 E049 0.7426990 0.0213006807 4.634470e-02 1.026573e-01 16 11796834 11797123 290 - 0.076 0.425 3.114
ENSG00000122299 E050 38.5076417 0.0070803352 7.645839e-05 4.112783e-04 16 11797124 11797258 135 - 1.625 1.321 -1.045