ENSG00000122008

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000241436 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding protein_coding 4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.98676849 0.00000000 1.54427198 0.00000000 0.06612167 7.2800952 0.16568750 0.0000000 0.209266667 0.20926667 1.257262e-03 2.963014e-09 FALSE TRUE
ENST00000505975 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding nonsense_mediated_decay 4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.02021818 0.08745353 0.05423856 0.08745353 0.05423856 -0.6012748 0.02847083 0.2024667 0.007500000 -0.19496667 5.571447e-01 2.963014e-09 FALSE TRUE
ENST00000508526 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding protein_coding 4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.01265745 0.08689239 0.00000000 0.08689239 0.00000000 -3.2763833 0.02369167 0.1880000 0.000000000 -0.18800000 2.275493e-01 2.963014e-09 FALSE TRUE
ENST00000509126 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding nonsense_mediated_decay 4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.02508162 0.05432752 0.00000000 0.05432752 0.00000000 -2.6854360 0.06613750 0.1175333 0.000000000 -0.11753333 1.585543e-01 2.963014e-09 TRUE TRUE
ENST00000514141 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding nonsense_mediated_decay 4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.01264503 0.04757839 0.04025856 0.04757839 0.04025856 -0.1961583 0.01787083 0.1101667 0.005033333 -0.10513333 4.734197e-01 2.963014e-09 TRUE TRUE
ENST00000515295 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding protein_coding 4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 2.06830260 0.00000000 3.88935534 0.00000000 0.32742137 8.6070918 0.26797083 0.0000000 0.523900000 0.52390000 2.963014e-09 2.963014e-09 FALSE TRUE
MSTRG.26423.10 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding   4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.05787993 0.00000000 0.11124127 0.00000000 0.11124127 3.5998089 0.08240000 0.0000000 0.015366667 0.01536667 8.813289e-01 2.963014e-09 FALSE TRUE
MSTRG.26423.2 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding   4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.29671070 0.00000000 0.51043691 0.00000000 0.05773051 5.7016514 0.06835417 0.0000000 0.069766667 0.06976667 1.812487e-01 2.963014e-09 TRUE TRUE
MSTRG.26423.4 ENSG00000122008 HEK293_OSMI2_2hA HEK293_TMG_2hB POLK protein_coding   4.271875 0.3996036 7.398593 0.04826702 0.3169566 4.176899 0.35550283 0.09518557 0.53527226 0.09518557 0.15609822 2.3740399 0.14610417 0.3124333 0.071000000 -0.24143333 9.913179e-01 2.963014e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000122008 E001 0.2987644 0.0295633093 1.0000000000   5 75511756 75511844 89 + 0.066 0.000 -8.827
ENSG00000122008 E002 0.2987644 0.0295633093 1.0000000000   5 75511845 75511856 12 + 0.066 0.000 -10.458
ENSG00000122008 E003 0.8942170 0.0138521203 1.0000000000 1.0000000000 5 75511857 75511890 34 + 0.174 0.000 -11.878
ENSG00000122008 E004 8.5732944 0.0355081798 0.0073644988 0.0221647477 5 75511891 75511914 24 + 0.760 0.000 -14.036
ENSG00000122008 E005 0.0000000       5 75512065 75512068 4 +      
ENSG00000122008 E006 0.4355181 0.5913240143 1.0000000000 1.0000000000 5 75512069 75512217 149 + 0.095 0.000 -10.200
ENSG00000122008 E007 1.3265129 0.0103088511 0.6990236803 0.7988558934 5 75512218 75512831 614 + 0.219 0.360 0.977
ENSG00000122008 E008 0.7480121 0.1288821136 0.3558716857 0.5002297800 5 75512832 75512946 115 + 0.123 0.358 1.964
ENSG00000122008 E009 2.5338773 0.0081946931 0.8007315536 0.8729861863 5 75534828 75534964 137 + 0.364 0.360 -0.022
ENSG00000122008 E010 9.0730189 0.0038371479 0.0365643329 0.0846488251 5 75547010 75547022 13 + 0.772 0.360 -1.930
ENSG00000122008 E011 17.5470703 0.0011042467 0.0573161831 0.1220124833 5 75547023 75547157 135 + 1.011 0.790 -0.843
ENSG00000122008 E012 0.1515154 0.0428831299 0.4892667100   5 75552471 75552471 1 + 0.034 0.000 -9.466
ENSG00000122008 E013 24.5493370 0.0012324305 0.0127828187 0.0354018155 5 75552472 75552591 120 + 1.147 0.873 -1.013
ENSG00000122008 E014 0.6653823 0.0171775403 0.2491841610 0.3849531344 5 75568677 75568705 29 + 0.096 0.360 2.394
ENSG00000122008 E015 1.1113958 0.0120020630 0.5284398843 0.6613597677 5 75568706 75568790 85 + 0.174 0.360 1.393
ENSG00000122008 E016 28.3338214 0.0012077559 0.0007479949 0.0030716044 5 75569340 75569492 153 + 1.211 0.790 -1.561
ENSG00000122008 E017 27.4611890 0.0006926815 0.0121780639 0.0339563704 5 75573738 75573869 132 + 1.193 0.942 -0.913
ENSG00000122008 E018 31.7859168 0.0039269996 0.0476880342 0.1050628563 5 75576780 75576933 154 + 1.249 1.101 -0.525
ENSG00000122008 E019 38.2612037 0.0033438043 0.0331689826 0.0781430289 5 75581209 75581448 240 + 1.324 1.182 -0.499
ENSG00000122008 E020 1.8079001 0.2372822615 0.8244018421 0.8893799516 5 75581449 75582037 589 + 0.279 0.361 0.522
ENSG00000122008 E021 1.4705276 0.0834002645 0.5350811336 0.6670231918 5 75582038 75582123 86 + 0.260 0.000 -12.257
ENSG00000122008 E022 1.7778249 0.0081333665 0.3820214985 0.5262789367 5 75582124 75582965 842 + 0.298 0.000 -12.644
ENSG00000122008 E023 27.6588025 0.0007180525 0.0035388090 0.0118366628 5 75583293 75583417 125 + 1.200 0.873 -1.201
ENSG00000122008 E024 31.4261667 0.0008222378 0.0216128352 0.0548726418 5 75584760 75584926 167 + 1.245 1.054 -0.681
ENSG00000122008 E025 18.1544142 0.0010771766 0.7042762149 0.8028502270 5 75587026 75587058 33 + 1.008 1.054 0.170
ENSG00000122008 E026 22.2488213 0.0008421892 0.9727757912 0.9870159636 5 75590344 75590440 97 + 1.085 1.182 0.350
ENSG00000122008 E027 0.5159433 0.2106149470 0.1731850276 0.2923327714 5 75590441 75590509 69 + 0.066 0.364 2.999
ENSG00000122008 E028 10.7807430 0.0018789161 0.4569280172 0.5972954616 5 75593878 75593965 88 + 0.790 1.002 0.808
ENSG00000122008 E029 17.0898560 0.0017608732 0.3397117375 0.4836390859 5 75593966 75594049 84 + 0.990 0.942 -0.178
ENSG00000122008 E030 92.9742213 0.0004137363 0.6890025831 0.7909870402 5 75596222 75597113 892 + 1.677 1.808 0.445
ENSG00000122008 E031 17.1266716 0.0021697527 0.0719067506 0.1465038951 5 75597114 75597178 65 + 0.960 1.281 1.155
ENSG00000122008 E032 0.2966881 0.0271734355 1.0000000000   5 75597417 75597746 330 + 0.066 0.000 -10.469
ENSG00000122008 E033 15.9905002 0.0012081903 0.7185966978 0.8136456865 5 75597747 75597789 43 + 0.952 1.101 0.547
ENSG00000122008 E034 0.3030308 0.3825930145 0.8224055571   5 75597790 75597933 144 + 0.066 0.000 -9.782
ENSG00000122008 E035 89.8927248 0.0708730020 0.0001400756 0.0007038964 5 75597934 75601144 3211 + 1.607 2.138 1.787