ENSG00000121989

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000241416 ENSG00000121989 HEK293_OSMI2_2hA HEK293_TMG_2hB ACVR2A protein_coding protein_coding 3.522453 1.369339 6.450583 0.1541765 0.5085024 2.227687 2.5063031 1.339233 3.530714 0.1375783 0.1405427 1.391901 0.76986667 0.9802667 0.5514 -0.4288667 1.400411e-12 1.400411e-12 FALSE TRUE
ENST00000404590 ENSG00000121989 HEK293_OSMI2_2hA HEK293_TMG_2hB ACVR2A protein_coding protein_coding 3.522453 1.369339 6.450583 0.1541765 0.5085024 2.227687 0.2123758 0.000000 0.000000 0.0000000 0.0000000 0.000000 0.06201250 0.0000000 0.0000 0.0000000   1.400411e-12 FALSE TRUE
ENST00000487959 ENSG00000121989 HEK293_OSMI2_2hA HEK293_TMG_2hB ACVR2A protein_coding processed_transcript 3.522453 1.369339 6.450583 0.1541765 0.5085024 2.227687 0.5791781 0.000000 2.527014 0.0000000 0.6032302 7.986988 0.08557083 0.0000000 0.3822 0.3822000 1.012937e-08 1.400411e-12   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000121989 E001 1.0330470 0.0119052897 5.730768e-02 1.219985e-01 2 147844517 147844523 7 + 0.395 0.000 -11.102
ENSG00000121989 E002 4.4414477 0.0036311298 1.135771e-05 7.451978e-05 2 147844524 147844615 92 + 0.867 0.000 -14.815
ENSG00000121989 E003 8.7355552 0.0019352497 1.310930e-04 6.635988e-04 2 147844616 147844706 91 + 1.088 0.544 -2.171
ENSG00000121989 E004 7.6576516 0.0149041155 3.832603e-01 5.274447e-01 2 147845029 147845073 45 + 0.947 0.814 -0.508
ENSG00000121989 E005 14.9391239 0.0357209783 6.729540e-02 1.388559e-01 2 147845074 147845207 134 + 1.244 0.984 -0.937
ENSG00000121989 E006 15.5370907 0.0477051335 9.747510e-02 1.866299e-01 2 147896301 147896363 63 + 1.259 1.009 -0.900
ENSG00000121989 E007 23.1145753 0.0009272806 5.793241e-03 1.806961e-02 2 147896364 147896508 145 + 1.426 1.190 -0.826
ENSG00000121989 E008 0.4375944 0.0277431456 3.411402e-01 4.850567e-01 2 147896509 147897114 606 + 0.214 0.000 -11.854
ENSG00000121989 E009 0.1472490 0.0446117713 1.000000e+00   2 147898241 147898330 90 + 0.084 0.000 -10.205
ENSG00000121989 E010 25.3264947 0.0280639435 2.700050e-01 4.085757e-01 2 147899458 147899567 110 + 1.444 1.298 -0.507
ENSG00000121989 E011 32.0639384 0.0031172534 5.141481e-01 6.489002e-01 2 147899744 147899898 155 + 1.519 1.461 -0.199
ENSG00000121989 E012 34.5809966 0.0006611910 7.147889e-01 8.108885e-01 2 147915191 147915334 144 + 1.541 1.505 -0.124
ENSG00000121989 E013 32.7440931 0.0007728613 4.555705e-01 5.960968e-01 2 147917283 147917426 144 + 1.530 1.469 -0.209
ENSG00000121989 E014 33.1937935 0.0006177863 8.244969e-02 1.633721e-01 2 147918447 147918592 146 + 1.481 1.573 0.316
ENSG00000121989 E015 29.0116271 0.0007580699 3.018347e-01 4.436574e-01 2 147920230 147920344 115 + 1.436 1.491 0.188
ENSG00000121989 E016 31.9314881 0.0044356973 5.246612e-01 6.581262e-01 2 147922973 147923111 139 + 1.487 1.517 0.104
ENSG00000121989 E017 2.5476044 0.0062802948 8.541853e-01 9.097969e-01 2 147925687 147926030 344 + 0.523 0.544 0.098
ENSG00000121989 E018 35.3157750 0.0050966628 9.798678e-01 9.914599e-01 2 147926031 147926161 131 + 1.543 1.530 -0.043
ENSG00000121989 E019 190.6462383 0.0003299072 3.069021e-06 2.290846e-05 2 147927080 147930826 3747 + 2.231 2.318 0.292