ENSG00000121864

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000326361 ENSG00000121864 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF639 protein_coding protein_coding 6.456985 2.249156 10.97806 0.1973284 0.8885148 2.282081 0.1442835 0.4191454 0.1274930 0.419145351 0.07240718 -1.642108 0.04500417 0.16316667 0.01123333 -0.151933333 0.9682054003 0.0004441322 FALSE TRUE
ENST00000483460 ENSG00000121864 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF639 protein_coding retained_intron 6.456985 2.249156 10.97806 0.1973284 0.8885148 2.282081 1.8802793 0.0635959 4.0174783 0.063595902 0.75598057 5.774108 0.20520833 0.02476667 0.36013333 0.335366667 0.0004441322 0.0004441322 FALSE FALSE
ENST00000491818 ENSG00000121864 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF639 protein_coding protein_coding 6.456985 2.249156 10.97806 0.1973284 0.8885148 2.282081 2.7154304 1.1971145 4.7374905 0.385431495 0.23740131 1.975603 0.46213750 0.54580000 0.44043333 -0.105366667 0.8074372128 0.0004441322 FALSE TRUE
ENST00000496856 ENSG00000121864 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF639 protein_coding protein_coding 6.456985 2.249156 10.97806 0.1973284 0.8885148 2.282081 0.5683866 0.2256433 1.1119092 0.006894397 0.18916815 2.251279 0.09292083 0.10136667 0.10333333 0.001966667 1.0000000000 0.0004441322 FALSE TRUE
MSTRG.24106.3 ENSG00000121864 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF639 protein_coding   6.456985 2.249156 10.97806 0.1973284 0.8885148 2.282081 0.1120274 0.1959128 0.0000000 0.195912847 0.00000000 -4.363962 0.03710417 0.10370000 0.00000000 -0.103700000 0.5593073477 0.0004441322 FALSE TRUE
MSTRG.24106.7 ENSG00000121864 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF639 protein_coding   6.456985 2.249156 10.97806 0.1973284 0.8885148 2.282081 0.4141698 0.0000000 0.3924319 0.000000000 0.20018337 5.330673 0.05217500 0.00000000 0.03403333 0.034033333 0.4721098680 0.0004441322 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000121864 E001 4.2279385 0.0112873018 2.060778e-01 3.337793e-01 3 179323031 179323075 45 + 0.720 0.530 -0.829
ENSG00000121864 E002 9.4952547 0.0227820229 8.365350e-03 2.470137e-02 3 179323076 179323151 76 + 1.045 0.662 -1.489
ENSG00000121864 E003 20.6306582 0.0211957532 8.054138e-03 2.391699e-02 3 179323152 179323245 94 + 1.340 1.051 -1.028
ENSG00000121864 E004 13.8528209 0.0405130034 9.936345e-02 1.895210e-01 3 179323246 179323247 2 + 1.165 0.947 -0.793
ENSG00000121864 E005 21.0109911 0.0296070428 4.941608e-02 1.081206e-01 3 179323248 179323291 44 + 1.333 1.118 -0.758
ENSG00000121864 E006 1.6544221 0.0082290588 1.987585e-01 3.246415e-01 3 179323763 179323844 82 + 0.443 0.204 -1.566
ENSG00000121864 E007 1.4801046 0.0089080774 2.523263e-01 3.886632e-01 3 179324153 179324261 109 + 0.414 0.203 -1.415
ENSG00000121864 E008 2.1799554 0.0962479770 8.436488e-01 9.026597e-01 3 179324950 179324993 44 + 0.470 0.447 -0.116
ENSG00000121864 E009 0.9286724 0.0158730785 6.625783e-01 7.708488e-01 3 179324994 179325041 48 + 0.275 0.204 -0.566
ENSG00000121864 E010 14.1699306 0.0013138986 6.497370e-01 7.608946e-01 3 179325042 179325045 4 + 1.127 1.113 -0.051
ENSG00000121864 E011 37.5629070 0.0012687514 3.168626e-01 4.596657e-01 3 179325046 179325152 107 + 1.540 1.507 -0.113
ENSG00000121864 E012 54.9220130 0.0079676376 6.528879e-01 7.635087e-01 3 179325153 179325322 170 + 1.690 1.691 0.003
ENSG00000121864 E013 46.5846108 0.0004750134 2.098234e-01 3.383446e-01 3 179327561 179327631 71 + 1.633 1.594 -0.133
ENSG00000121864 E014 1.0351207 0.0121476133 1.792406e-01 3.001688e-01 3 179327744 179327906 163 + 0.185 0.447 1.757
ENSG00000121864 E015 2.0109234 0.0160228909 4.398233e-01 5.815573e-01 3 179328118 179328282 165 + 0.470 0.342 -0.704
ENSG00000121864 E016 26.9767954 0.0009777747 6.496858e-01 7.608671e-01 3 179328283 179328293 11 + 1.396 1.396 0.000
ENSG00000121864 E017 48.2238216 0.0011839412 8.295416e-02 1.641691e-01 3 179328294 179328351 58 + 1.656 1.587 -0.233
ENSG00000121864 E018 42.2233590 0.0005421025 7.433879e-02 1.504858e-01 3 179329618 179329633 16 + 1.600 1.522 -0.264
ENSG00000121864 E019 79.3449195 0.0003447369 1.398711e-01 2.478509e-01 3 179329634 179329728 95 + 1.860 1.828 -0.105
ENSG00000121864 E020 3.2785509 0.0047100447 6.585455e-01 7.676467e-01 3 179329934 179330055 122 + 0.563 0.662 0.434
ENSG00000121864 E021 113.4385416 0.0002720524 4.909658e-02 1.075420e-01 3 179332989 179333123 135 + 2.013 1.975 -0.129
ENSG00000121864 E022 553.8217226 0.0028323951 4.559465e-07 4.064259e-06 3 179333269 179338583 5315 + 2.655 2.775 0.401