ENSG00000121753

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000398538 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding protein_coding 9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 0.5395972 0.6550800 0.5173702 0.3857154 0.29033408 -0.3347120 0.05796667 0.04596667 0.07460000 0.02863333 9.397390e-01 3.52253e-14 FALSE TRUE
ENST00000398542 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding protein_coding 9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 2.4402529 3.4927827 1.2567805 0.5409494 0.37556890 -1.4673349 0.24919167 0.24986667 0.18940000 -0.06046667 7.174203e-01 3.52253e-14 FALSE TRUE
ENST00000398556 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding protein_coding 9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 0.7411444 1.3300370 0.3464359 0.3937000 0.34643594 -1.9105581 0.07143750 0.09403333 0.05003333 -0.04400000 3.016467e-01 3.52253e-14 FALSE TRUE
ENST00000465256 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding retained_intron 9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 1.1398752 0.9124844 1.0066788 0.1916450 0.08902702 0.1402677 0.12402917 0.06420000 0.14930000 0.08510000 4.624171e-03 3.52253e-14 FALSE TRUE
ENST00000527361 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding protein_coding 9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 0.4809981 0.0000000 0.5122722 0.0000000 0.30078368 5.7067300 0.06130417 0.00000000 0.07616667 0.07616667 1.168750e-01 3.52253e-14 FALSE TRUE
ENST00000530999 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding retained_intron 9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 0.9416087 3.0831969 0.0000000 0.4212780 0.00000000 -8.2729549 0.07170833 0.22480000 0.00000000 -0.22480000 3.522530e-14 3.52253e-14 FALSE TRUE
MSTRG.742.10 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding   9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 1.6033744 2.8743816 0.8049619 0.4851623 0.14486727 -1.8234575 0.15742083 0.20556667 0.11906667 -0.08650000 1.992951e-01 3.52253e-14 FALSE TRUE
MSTRG.742.22 ENSG00000121753 HEK293_OSMI2_2hA HEK293_TMG_2hB ADGRB2 protein_coding   9.755407 13.97938 6.721 0.7155356 0.219395 -1.055439 0.1678136 0.0000000 0.5524594 0.0000000 0.11381689 5.8136770 0.01753750 0.00000000 0.08196667 0.08196667 1.774711e-11 3.52253e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000121753 E001 1.9510164 0.0075633711 3.636516e-01 5.080770e-01 1 31727117 31727117 1 - 0.299 0.466 0.957
ENSG00000121753 E002 27.4498745 0.0037226227 1.340310e-05 8.644008e-05 1 31727118 31727169 52 - 1.093 1.477 1.348
ENSG00000121753 E003 35.3749811 0.0092962660 8.885370e-04 3.574188e-03 1 31727170 31727194 25 - 1.286 1.570 0.978
ENSG00000121753 E004 44.9111331 0.0127851189 8.315915e-05 4.429706e-04 1 31727195 31727243 49 - 1.339 1.679 1.167
ENSG00000121753 E005 82.5525578 0.0065968318 2.185319e-10 3.633510e-09 1 31727244 31727395 152 - 1.534 1.945 1.393
ENSG00000121753 E006 42.3070155 0.0006794660 3.803676e-06 2.781575e-05 1 31727396 31727410 15 - 1.339 1.645 1.052
ENSG00000121753 E007 44.9078722 0.0019001192 1.225738e-05 7.978433e-05 1 31727411 31727424 14 - 1.377 1.667 0.997
ENSG00000121753 E008 144.6158470 0.0066300635 7.051328e-03 2.135759e-02 1 31727425 31727605 181 - 2.003 2.141 0.462
ENSG00000121753 E009 18.7057238 0.0024322463 2.163195e-06 1.669631e-05 1 31727606 31728024 419 - 1.481 1.092 -1.366
ENSG00000121753 E010 44.3355293 0.0006793058 3.462084e-01 4.903603e-01 1 31728025 31728081 57 - 1.564 1.617 0.178
ENSG00000121753 E011 133.0124860 0.0028435034 7.221047e-03 2.179808e-02 1 31728182 31728277 96 - 1.985 2.099 0.381
ENSG00000121753 E012 46.3411740 0.0013972277 7.885707e-01 8.646342e-01 1 31728278 31728280 3 - 1.609 1.622 0.043
ENSG00000121753 E013 80.3972897 0.0029949575 1.452628e-01 2.552011e-01 1 31728598 31728633 36 - 1.802 1.874 0.243
ENSG00000121753 E014 281.0307106 0.0011303069 2.143192e-02 5.449877e-02 1 31730800 31731419 620 - 2.347 2.408 0.203
ENSG00000121753 E015 63.2082524 0.0119127359 6.430952e-01 7.557095e-01 1 31732115 31732154 40 - 1.770 1.739 -0.107
ENSG00000121753 E016 89.9675987 0.0005743676 5.818570e-01 7.063377e-01 1 31732517 31732612 96 - 1.883 1.903 0.068
ENSG00000121753 E017 108.9704263 0.0003594680 8.967003e-01 9.379831e-01 1 31732972 31733143 172 - 1.978 1.980 0.006
ENSG00000121753 E018 15.8207346 0.0016734152 3.488398e-01 4.930588e-01 1 31735183 31735281 99 - 1.110 1.197 0.310
ENSG00000121753 E019 1.2586699 0.0157093974 6.537463e-02 1.356669e-01 1 31735282 31735579 298 - 0.541 0.215 -1.952
ENSG00000121753 E020 89.4858737 0.0011677958 4.263338e-01 5.691806e-01 1 31735580 31735665 86 - 1.874 1.906 0.108
ENSG00000121753 E021 76.4396517 0.0003530374 6.611777e-02 1.369110e-01 1 31735827 31735893 67 - 1.767 1.848 0.274
ENSG00000121753 E022 75.0090012 0.0004050421 6.924338e-01 7.936853e-01 1 31736321 31736390 70 - 1.809 1.824 0.050
ENSG00000121753 E023 16.2611321 0.0475646295 3.455447e-03 1.159673e-02 1 31736391 31736572 182 - 1.419 1.040 -1.340
ENSG00000121753 E024 83.2546002 0.0003629397 3.703815e-01 5.148183e-01 1 31736573 31736723 151 - 1.838 1.874 0.120
ENSG00000121753 E025 76.4934038 0.0003774821 4.257529e-01 5.686641e-01 1 31737429 31737531 103 - 1.857 1.818 -0.129
ENSG00000121753 E026 63.0443121 0.0005088211 9.022993e-01 9.417599e-01 1 31737652 31737755 104 - 1.748 1.751 0.009
ENSG00000121753 E027 54.1357815 0.0005434858 9.553810e-01 9.760039e-01 1 31738200 31738244 45 - 1.682 1.682 -0.001
ENSG00000121753 E028 64.4005928 0.0004320675 6.857940e-01 7.884311e-01 1 31738245 31738326 82 - 1.771 1.748 -0.077
ENSG00000121753 E029 53.8071697 0.0004755591 3.885219e-01 5.326042e-01 1 31738587 31738630 44 - 1.712 1.664 -0.164
ENSG00000121753 E030 73.7429946 0.0023135473 2.376628e-01 3.714454e-01 1 31738832 31738937 106 - 1.856 1.796 -0.203
ENSG00000121753 E031 1.5167355 0.0111786185 2.716752e-02 6.633762e-02 1 31738938 31739210 273 - 0.599 0.215 -2.211
ENSG00000121753 E032 96.5931127 0.0003956733 1.804434e-02 4.722916e-02 1 31739308 31739514 207 - 1.998 1.904 -0.318
ENSG00000121753 E033 72.9126935 0.0075100841 4.920334e-02 1.077410e-01 1 31739515 31739635 121 - 1.894 1.775 -0.400
ENSG00000121753 E034 61.6880488 0.0036555368 6.529428e-02 1.355298e-01 1 31739926 31740034 109 - 1.809 1.706 -0.348
ENSG00000121753 E035 34.7286850 0.0005730565 1.490075e-02 4.026218e-02 1 31740110 31740178 69 - 1.604 1.449 -0.528
ENSG00000121753 E036 76.8495416 0.0011295125 2.330497e-02 5.838400e-02 1 31740347 31740541 195 - 1.907 1.803 -0.353
ENSG00000121753 E037 55.6456173 0.0006593602 8.260034e-02 1.636125e-01 1 31741373 31741479 107 - 1.760 1.668 -0.311
ENSG00000121753 E038 53.0625875 0.0103973203 1.529983e-02 4.116740e-02 1 31741624 31741725 102 - 1.792 1.625 -0.566
ENSG00000121753 E039 62.8490705 0.0122512639 4.096410e-01 5.532445e-01 1 31741800 31741967 168 - 1.785 1.731 -0.183
ENSG00000121753 E040 35.4566811 0.0014896780 2.946959e-01 4.358119e-01 1 31742053 31742107 55 - 1.553 1.482 -0.243
ENSG00000121753 E041 47.9532697 0.0092785161 1.663754e-01 2.834806e-01 1 31742108 31742217 110 - 1.700 1.603 -0.328
ENSG00000121753 E042 7.9877883 0.0138021503 1.089177e-01 2.039390e-01 1 31742838 31743002 165 - 1.038 0.832 -0.774
ENSG00000121753 E043 0.2966881 0.0294275323 5.629167e-02   1 31743459 31743673 215 - 0.299 0.000 -10.363
ENSG00000121753 E044 45.7848330 0.0174242537 2.790195e-01 4.187215e-01 1 31744193 31744357 165 - 1.673 1.588 -0.288
ENSG00000121753 E045 44.8162530 0.0213722718 5.692931e-02 1.213387e-01 1 31744648 31744731 84 - 1.716 1.554 -0.552
ENSG00000121753 E046 61.3797595 0.0090257637 4.259259e-03 1.386766e-02 1 31755999 31756371 373 - 1.863 1.679 -0.621
ENSG00000121753 E047 38.6893590 0.0100329161 2.176886e-03 7.770369e-03 1 31756372 31756572 201 - 1.696 1.466 -0.783
ENSG00000121753 E048 42.5532103 0.0161756553 1.132291e-03 4.412133e-03 1 31756573 31756815 243 - 1.764 1.495 -0.916
ENSG00000121753 E049 3.5460307 0.0878064007 3.730508e-02 8.604536e-02 1 31757201 31757216 16 - 0.872 0.490 -1.624
ENSG00000121753 E050 4.5434869 0.0116794593 7.953386e-03 2.365936e-02 1 31757217 31757281 65 - 0.951 0.576 -1.518
ENSG00000121753 E051 5.2755622 0.0030818791 5.264260e-02 1.138607e-01 1 31757382 31757511 130 - 0.926 0.666 -1.033
ENSG00000121753 E052 1.1888117 0.8700653265 7.914426e-01 8.665994e-01 1 31759321 31759423 103 - 0.301 0.293 -0.051
ENSG00000121753 E053 0.5483223 0.0210506457 9.098428e-01 9.467072e-01 1 31760683 31761026 344 - 0.175 0.155 -0.211
ENSG00000121753 E054 23.5974323 0.0206858089 4.797673e-02 1.055963e-01 1 31763884 31764340 457 - 1.466 1.273 -0.671
ENSG00000121753 E055 0.0000000       1 31764680 31764893 214 -